Fasting venous blood was centrifuged at 4000 rpm for 10 min to obtain serum. All laboratory assessments were performed at baseline and weeks 4, 12, 24, 48, and 96. HBV DNA was detected by quantitative polymerase chain reaction (PCR) using the Roche COBAS AmpliPrep/COBAS TaqMan system (Roche Diagnostics, Basel, Switzerland). The lowest detection limit was 20 IU/mL. Hepatitis B surface antigen (HBsAg), anti-HBs, HBeAg, anti-HBe, and anti-HBc were detected by chemiluminescence microparticle immunoassays using the Architect i2000SR platform and Abbott Architect reagents (Abbott Laboratories, Abbott Park, IL, United States). Serum HBsAg levels were measured with a dynamic range of 0-250 IU/mL. If qHBsAg levels were > 250 IU/mL, the samples were retested with a stepwise dilution of 1:10,000. ALT and aspartate aminotransferase were measured at each participating medical site. ALT, HBsAg, HBeAg, and HBV DNA were directly detected immediately at each time point. The HBV genotype was determined at screening. HBV pgRNA was measured at Peking University Health Science Center (Beijing, China), as previously described[25 (link)]. HBV genotypes were determined by real-time PCR with Taqman probe technology (Shanghai ZJ Bio-Tech, Shanghai, China). A FibroScan system was used to measure liver stiffness (Echosens, Paris, France).
Fibroscan system
Manufactured by Echosens
Sourced in France
The FibroScan system is a non-invasive diagnostic device used to assess the stiffness of the liver. It utilizes ultrasound technology to measure liver stiffness, providing healthcare professionals with quantitative data to aid in the evaluation of liver conditions.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using fibroscan system
1
Evaluating HBV Biomarkers in Chronic Hepatitis
2
Transient Elastography for Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transient elastography (TE, Echosens, Paris, France) was performed with the Fibroscan® system using the M probe. The examinations were carried out by a well-trained radiologic technician (with an experience of more than 1,000 cases of TE LSM) blinded to ARFI and SSI results and histological data. As previously described [11 (link), 20 (link)], poorly reliable or unreliable data were defined as an interquartile range (IQR) per median of LSM (IQR/M) > 0.3 with a median LSM ≥ 7.1 kPa, and those unreliable results were excluded from analysis.
3
Liver Fibrosis Evaluation by FibroScan
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LS was examined through TE by the FibroScan system (Echosens, Paris, France) at M0 and M12. A minimum of 10 LS measurements were required. The median value was assumed to be representative of LS. A set of measurements was considered to be reliable if the success rate was ≥ 60% and the interquartile range was less than one-third of the median LS value. Unreliable measurements of LS were excluded. All measurements were obtained by two trained operators (MRP and JAGG) using a single device. Results, expressed in kilopascals (kPa), were categorized in three stages as follows: non-significant fibrosis (F0–F1), under 7.81 kPa; significant fibrosis (F2–F3), equal or more than 7.81 kPa; cirrhosis (F4), ≥ 15.56 kPa, according to Stebbing’s meta-analysis40 (link). Besides fibrosis, other factors may also result in increased liver stiffness, such as the presence of severe hepatic inflammation, extrahepatic arteriovenous or biliary obstruction, and congestive heart failure24 (link). They were excluded by clinical and complementary explorations.
AST to platelets ratio index (APRI)20 (link) and Fibrosis-4 (FIB-4) score21 (link) were also calculated.
AST to platelets ratio index (APRI)20 (link) and Fibrosis-4 (FIB-4) score21 (link) were also calculated.
4
Liver Stiffness Measurement with FibroScan
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TE was performed with a FibroScan system (Echosens, Paris, France) using the M probe. All LSMs were performed under fasting conditions within 3 days of LB by experienced operators according to the manufacturer's protocol. Operators were blinded to the clinical data and pathology results. The value expressed in kilopascal (kPa) was recorded as a representation of the LSM. Up to 10 valid measurements were performed on each patient. A success rate above 60% and an interquartile range/median ratio of less than 30% were considered reliable [20 (link)].
5
Liver Stiffness Measurement by FibroScan
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TE was performed with a FibroScan system (Echosens, Paris, France) using the M probe. After an overnight fast, patients underwent TE examination within 3 days of LB by trained operators who had previously performed at least 500 scans in patients with chronic liver disease. Operators were blinded to the clinical data and pathology results. The value expressed in kilopascal (kPa) was recorded as a representation of the LSM. Up to 10 valid measurements were performed on each patient. A success rate above 60% and an interquartile range/median ratio of less than 30% were considered reliable [20 (link)].
6
Non-Invasive Liver Fibrosis Staging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver stiffness measurement (LSM) was performed using the FibroScan system (Echosens, Paris, France) as a non-invasive method to estimate the severity of liver fibrosis. The range of results is expressed between 2.5 and 75 kPa. According to the LSM, F0–F4 were defined as: < 7.3 kPa for no/mild fibrosis (F0/F1), ≥ 7.3 and < 9.7 kPa for moderate fibrosis (F2), ≥ 9.7 and < 12.4 kPa for severe fibrosis (F3) and ≥ 12.4 kPa for cirrhosis (F4) [14 (link)].
7
Transient Elastography for Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver stiffness was examined through transient elastography by the FibroScan system (Echosens, Paris, France) in every clinical center at base line and after the EOT. Results, expressed in kilopascals (kPa), were categorized in four Metavir stages as follows: (F1) under 7.1 kPa; (F2) from 7.1 to 9.5 kPa; (F3) from 9.5 to 12.4 kPa; (F4) above 12.4 kPa. It has been demonstrated that transient elastography's best performance is for severe fibrosis or cirrhosis, with the 12.5 kPa cut-off showing positive and negative predictive values for biopsy-proven cirrhosis of 77% and 95%, respectively [11] .
The APRI and FIB-4 scores were calculated at baseline, EOT, and W12. For the APRI score, we used the predefined thresholds of 0.77 for advanced fibrosis (Metavir F3 or F4) [12] and 0.84 for cirrhosis, and for FIB-4, we used 1.45 points to reject cirrhosis (negative predictive value, 90%) [13] .
The APRI and FIB-4 scores were calculated at baseline, EOT, and W12. For the APRI score, we used the predefined thresholds of 0.77 for advanced fibrosis (Metavir F3 or F4) [12] and 0.84 for cirrhosis, and for FIB-4, we used 1.45 points to reject cirrhosis (negative predictive value, 90%) [13] .
8
Transient Elastography Liver Stiffness Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transient Elastography was performed in all patients using the FibroScan system (EchoSens, Paris, France), using the M probe (standard probe -transducer frequency 3.5 MHz). The examinations were performed according to the EFSUMB and WFUMB guidelines [13, (link)14] (link): all patients fasted for at least 3 hours before the elastographic measurements were performed, with each patient in a supine position, right arm in maximum abduction, by intercostal approach, in the right liver lobe. In each patient, we aimed for 10 valid liver stiffness (LS) measurements. The median value of 10 valid LS measurements was calculated and the results were expressed in kilopascals (kPa). Reliable measurements were defined as the median value of 10 valid LS measurements with an interquartile range interval/median ratio (IQR/M) <30% [13, (link)14] (link). Patients with invalid/unreliable measurements were excluded from the study.
9
Comprehensive Anthropometric and Sonographic Assessment of NAFLD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anthropometric measurements were performed by a research physician with the assistance of a staff nurse using standard protocols and calibrated instruments. The weight and height of participants were measured with light clothes and without shoes. BMI was calculated as weight (kg) divided by height (m)2. WC was measured with a nonelastic tape at a point midway between the lower border of the rib cage and the iliac crest at the end of normal expiration. All laboratory tests were conducted at the biochemistry and pathology department of the university. The sonographic feature of NAFLD was determined based on the presence of bright hepatic echotexture (compared with kidney), blurring of intrahepatic vasculature, and deep attenuation, either singly or in combination. All sonography was performed by a single experienced radiologist. Elastography scans were also performed by an expert elastographer with the FibroScan system (Echosens, Paris, France), using the M probe for standard examinations and the XL probe to increase the reliability of measurements in overweight patients.
10
Transient Elastography Liver Stiffness Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
Transient elastography was performed with the FibroScan system (Echosens, Paris, France) using the M probe after overnight fasting. Measurements were performed by a gastroenterologist (H.P-L.) with experience in transient elastography. The stiffness of the tissue was measured in kilopascals (kPa), with a minimum of 10 valid readings per patient; a ≥ 60 % success rate and an interquartile range ≤ 30 % were considered to indicate adequate quality of the assessment
11 (link)
.
+ Expand
Transient elastography was performed with the FibroScan system (Echosens, Paris, France) using the M probe after overnight fasting. Measurements were performed by a gastroenterologist (H.P-L.) with experience in transient elastography. The stiffness of the tissue was measured in kilopascals (kPa), with a minimum of 10 valid readings per patient; a ≥ 60 % success rate and an interquartile range ≤ 30 % were considered to indicate adequate quality of the assessment
11 (link)
.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!