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6 protocols using sodium citrate

1

Corn Cob Acid Hydrolysis Protocol

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Corn cob was provided by Abhyoday feedstock suppliers, Jalgaon, India. These are air-dried and milled into small particles (3.5 mm × 2.5 mm) before acid hydrolysis. Aminex column HPX87H (300 mm × 7.8 mm) was purchased from Biorad, Hercules, CA, USA. All used chemicals were of analytical grade. Sodium alginate, calcium chloride and sodium citrate were procured from Himedia, Mumbai.
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2

Diabetic Rat Model Protocols

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Ethanol and normal saline (0.9% NaCl) were obtained from the Central Medical Store of the Institution. Human Neutral Protamine Hagedorn (NPH) insulin, STZ, thiobarbituric acid, citric acid, sodium citrate, sodium hydroxide, and glucose estimation kits were obtained from Hi Media Laboratories Pvt., Limited, Mumbai, Maharashtra, India. Kits for estimation of lipid profile were obtained from Crest Biosystems, Goa, India. Ether, thiopentone sodium, potassium phosphate buffer, hydrogen peroxide solution, and tricarboxylic acid were obtained from Sigma-Aldrich India, Bengaluru, Karnataka, India. Crude powder of metformin was obtained from Aventis Pharma Limited, Goa, India. RIA kits for insulin assay were supplied by Board of Radiation and Isotope Technology, Bhabha Atomic Research Centre (Navi Mumbai, Maharashtra, India).
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3

Fabrication of Asymmetric Membranes with Antimicrobial Properties

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Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
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4

Casein and Gelatin Protocol

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Casein was purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Gelatin, pure amino acids (L form), Minimal Davis Broth (Ingredients g/L: Dipotassium phosphate, 7.0 g; Monopotassium phosphate, 2.0 g; Sodium citrate, 0.5 g; Magnesium sulphate, 0.1 g; Ammonium sulphate,1.0 g) and other media components were purchased from Hi-Media Laboratories (Mumbai, India). All other chemicals used were of highest purity grade.
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5

Green Synthesis of Gold Nanoparticles

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We procured chloroauric acid (HAuCl4) and sodium citrate from HiMedia Laboratories Pvt., Ltd. (Maharashtra, India). We bought polyvinylpropylene from Sigma-Aldrich, USA. C. verum bark was procured from local market. We received ELISA kits of IL-1β, IL-6, INF-γ, VEGF, and RANTES from R & D Systems, Canada. CD36, FAS, FABP4, PPARγ, and ACC antibodies were procured from Thermo Fisher Scientific and Abcam.
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6

Fabrication of Asymmetric Membranes with Antimicrobial Properties

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Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
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