Metromix 360 soil

Manufactured by Sun Gro Horticulture

Sourced in United States

Metromix 360 is a professional-grade soil mix formulated by Sun Gro Horticulture. It is a peat-based growing medium designed for use in greenhouse and nursery settings.

Automatically generated - may contain errors

Lab products found in correlation

Ms salt, by Merck Group (1 mentions) Eos rebel t3 camera, by Canon (1 mentions)

6 protocols using metromix 360 soil

Arabidopsis Natural Accessions and Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols

A. thaliana natural accessions used in this study were obtained from the Arabidopsis Biological Resource Center (Ohio, USA). A. lyrata seed was provided by O. Savolainen (Oulu, Finland). The co-1-mutant line that is introgressed in Col-0 was provided by G. Coupland (Koln, Germany). The FRI Sf2 line carrying the FRI locus that is introgressed from Sf2 into Col-0, and the FRI Sf2 co-1 line carrying the FRI locus from Sf2 and the co-1 mutation were provided by R. Amasino (Madison, USA).
Plants were grown under continuous fluorescent light, long-day (14L:10D) or short-day (10L:14D) conditions at 21 °C on Metromix 360 soil (SUNGRO Horticulture, USA). For screening transgenic plants, plants were grown under continuous fluorescent light on 1.0% (w/v) agar plates containing MS salts (Sigma), 2% sucrose and 50 μg ml⁻¹ kanamycin after sterilization of the surface of the seeds.

Rosas U., Mei Y., Xie Q., Banta J.A., Zhou R.W., Seufferheld G., Gerard S., Chou L., Bhambhra N., Parks J.D., Flowers J.M., McClung C.R., Hanzawa Y, & Purugganan M.D. (2014). Variation in Arabidopsis flowering time associated with cis-regulatory variation in CONSTANS. Nature Communications, 5, 3651.

+ Open protocol

+ Expand

Salicornia Seed Germination and Salt Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

Salicornia bigelovii seeds were kindly provided by Dr. E. Glenn of the Environmental Research Laboratory, University of Arizona, Tucson, USA. Salicornia europaea seeds were collected in the Dead Sea area and were kindly provided by Dr. Moshe Sagi of the Blaustein Institute for Desert Research (BIDR), Israel. For phenotypic, transcriptomic, and proteomic experiments in S. bigelovii, seeds derived from inbred populations were sown in Metro-Mix® 360 soil (SunGro Horticulture, USA) contained in plastic trays and watered daily to 70% soil water holding capacity. S. bigelovii plants of one inbred line were grown during the months of May–July 2015 in a glasshouse in KAUST under natural irradiance and kept at a constant temperature of 28/24 °C day/night with 65% relative humidity. Five weeks after sowing, S. bigelovii plants were treated with saline water to reach a final concentration of 0, 5, 50, 200, or 600 mM NaCl in the soil. Three trays per treatment, for a total of 12 trays, were grown for the experiments with all replicates showing consistent phenotypes.

Salazar O.R., Chen K., Melino V.J., Reddy M.P., Hřibová E., Čížková J., Beránková D., Arciniegas Vega J.P., Cáceres Leal L.M., Aranda M., Jaremko L., Jaremko M., Fedoroff N.V., Tester M, & Schmöckel S.M. (2024). SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii. Nature Communications, 15, 4279.

+ Open protocol

+ Expand

Plant Growth in Controlled Environment

Check if the same lab product or an alternative is used in the 5 most similar protocols

All plants were grown in Metro-Mix 360 soil (Sun-Gro Horticulture City State) in growth chambers under a 16-h light/8-h dark photoperiod, at 22 °C and 50% of humidity.

Huang J., Zhao L., Malik S., Gentile B.R., Xiong V., Arazi T., Owen H.A., Friml J, & Zhao D. (2022). Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis. Nature Communications, 13, 6960.

+ Open protocol

+ Expand

Agrobacterium-Mediated Transformation of Arabidopsis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Arabidopsis thaliana, ecotype Columbia-0 (Col-0) was used as the parent line for Agrobacterium-mediated transformation with the AcCel5A gene constructs. Col-0 seeds were germinated on ½ MS agar medium containing 1% sucrose. After 2-d incubation for cold stratification at 4 °C, the medium plates were kept in a growth chamber with 16 h light (140 µmol E m⁻² s⁻¹) and 8 h dark cycles at 24 °C for 5 d, then the seedlings were transferred to pots that contained Metro-Mix 360 soil (SunGro Horticulture, Canada) and kept under light shelves of ArabiSun Lighting System (Lehle seeds, Texas, USA) with 16 h light (170 µmol E m⁻² s⁻¹) and 8 h dark cycles at 24 °C. Transgenic and empty vector control seedlings were randomly placed in the greenhouse and watered with distilled H₂O twice a week, with 16 h light (200 to 300 µmol E m⁻² s⁻¹), 8 h dark cycles at 24 °C. Plants were harvested at the senescent stage as described previously²⁸. Briefly, the naturally dried shoot samples were then knife-milled and passed through a 20-mesh (1 mm) screen twice for uniformity (Wiley knife mill; Thomas-Wiley, Philadelphia).

Donohoe B.S., Wei H., Mittal A., Shollenberger T., Lunin V.V., Himmel M.E, & Brunecky R. (2017). Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase. Scientific Reports, 7, 4389.

+ Open protocol

+ Expand

Shoot Branch Phenotypes in Continuous Light

Check if the same lab product or an alternative is used in the 5 most similar protocols

For shoot branch phenotypes, seedlings were grown for 2 weeks on plates, then transplanted into 4 inch pots containing Metromix 360 soil (Sun-Gro Horticulture, http://www.sungro.com) and grown until bolting (~15-18 cm in height). Plants were then transferred to continuous light or dark conditions for 72 h. Images were taken using a Canon EOS Rebel T3 camera (http://global.canon/en/index.html, last accessed 27 July 2018).

Waite J.M., & Dardick C. (2020). IGT/LAZY family genes are differentially influenced by light signals and collectively required for light-induced changes to branch angle.

+ Open protocol

+ Expand

Arabidopsis Genetic Mutant Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The csn2 (fus12-U228), csn4 (cop8-1), cop1-5, and csn5a-2 mutants are in the Arabidopsis thaliana Landsberg erecta (Ler), Wassilewskija (Ws) (csn4 and cop1-5), and Columbia (Col) accessions, respectively (McNellis et al., 1994; Misera et al., 1994; Wei et al., 1994; Gusmaroli et al., 2004; Dohmann et al., 2005) . pSTM:GUS, M0233, pSCR:GFP, QC25, QC46, pWOX5:GFP, DR5rev:GFP, pPIN1:PIN1-GFP, have been described previously (Sabatini et al., 1999; Wysocka-Diller et al., 2000; Cary et al., 2002; Friml et al., 2003; Blilou et al., 2005; Gordon et al., 2007; Jurkuta et al., 2009) and were crossed into the CSN2/csn2 heterozygous mutant. Q1630 is from the Haseloff collection (http://arabidopsis.info/ CollectionInfo?id=24). Unless otherwise noted, Arabidopsis seedlings were surface-sterilized and grown on solid Murashige and Skoog medium with Gamborg's vitamins (Duchefa) and 1% sucrose at 22 C with a light intensity of 130 mmol$m À2 $s À1 . For crosses and the analysis of embryos, plants were grown in MetroMix 360 soil (SunGro Horticulture) in a greenhouse at 22-24 C with continuous light.

Franciosini A., Moubayidin L., Du K., Matari N.H., Boccaccini A., Butera S., Vittorioso P., Sabatini S., Jenik P.D., Costantino P, & Serino G. (2015). The COP9 SIGNALOSOME Is Required for Postembryonic Meristem Maintenance in Arabidopsis thaliana. Molecular plant, 8(11).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!