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Rabbit anti ho 1

Manufactured by Enzo Life Sciences
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Rabbit anti-HO-1 is a primary antibody that recognizes the Heme Oxygenase-1 (HO-1) protein. HO-1 is an enzyme involved in the breakdown of heme. This antibody can be used to detect and quantify HO-1 expression in various biological samples.

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Lab products found in correlation

Recombinant human vegf a, by R&D Systems (2 mentions) Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Basement membrane matrix, by BD (2 mentions) Anti phospho fak y397, by Abcam (2 mentions) Mouse anti phospho erk1 2 y204, by Santa Cruz Biotechnology (2 mentions) Rabbit anti nrf2 antibody, by Abcam (1 mentions) Mouse anti gapdh antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti histone h3 antibody, by Abcam (1 mentions) Anti rabbit monoclonal, by GE Healthcare (1 mentions) Triton x 100, by Merck Group (1 mentions) Mouse anti neun, by Merck Group (1 mentions) Aβ25 35 peptide, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Dulbecco s modified eagle medium dmem, by Cytiva (1 mentions) 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions) Mouse anti syn, by Merck Group (1 mentions) Anhydrous dimethyl sulfoxide dmso, by Merck Group (1 mentions) Rabbit anti psd95, by Abcam (1 mentions) Goat anti nqo1, by Abcam (1 mentions) Normal goat serum ngs, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Anti-HO-1 (18 citations) Rabbit anti-HO-1 polyclonal antibody (5 citations) Rabbit anti-HO-1 antibody (3 citations) Rabbit anti-human HO-1 antibody (2 citations) Polyclonal rabbit anti-HO-1 (2 citations) Rabbit anti-HO-1 Ab (1 citations)

8 protocols using rabbit anti ho 1

1

Western Blot Analysis of Nrf2 and HO1

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We performed Western blot analysis of erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO1) proteins in homogenates derived from irradiated EpidermFT tissues (Fig. 3). Nuclear and cytoplasmic proteins were fractionated and Nrf2 was probed in the nuclear fraction using rabbit anti-Nrf2 antibody (1:250) and the housekeeping gene histone H3 was probed using rabbit anti-histone H3 antibody (1:3,000) (both from Abcam®, Cambridge, MA). HO1 was probed in the cytoplasmic fraction using rabbit anti-HO1 (1:500; Enzo Life Sciences Inc., Farmingdale, NY) and GAPDH was detected using mouse anti-GAPDH antibody (1:5,000; Thermo Fisher Scientifice™ Inc., Waltham, MA). The secondary antibody used was anti-rabbit monoclonal (1:5,000; GE Healthcare Bio-Sciences, Pittsburgh, PA) or anti-mouse monoclonal (1:5,000).
We performed Western blot analysis of heme oxygenase 1 (HO1) protein in homogenates derived from nonirradiated EpidermFT tissue (Fig. 4). Dermaprazole (1–2%) was applied on the tissue topically and the viable EpidermFT tissue was incubated at 378C/5% CO2 for 24 h. HO1 was probed using rabbit anti-HO1 (1:500; Enzo Life Sciences) and GAPDH was detected using mouse anti-GAPDH antibody (1:5,000; Thermo Fisher Scientific). The secondary antibody used was anti-rabbit monoclonal (1:5,000; GE Healthcare).
Pham N., Ludwig M.S., Wang M., Ebrahimpour A., Bonnen M.D., Diwan A.H., Kim S.J., Bryan J., Newton J.M., Sikora A.G., Donovan D.T., Sandulache V, & Ghebre Y.T. (2019). Topical Esomeprazole Mitigates Radiation-Induced Dermal Inflammation and Fibrosis. Radiation research, 192(5), 473-482.
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2

Alzheimer's Model Neuroprotective Assays

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Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin (PS) were purchased from Hyclone Laboratories (Logan, UT, USA). Aβ25–35 peptide, dimethylsulfoxide anhydrous (DMSO), mouse anti-SYN, triton X-100, slide mounting medium for histology, paraformaldehyde (PFA), 3,3-diaminobenzidine (DAB), DPPH, ABTS, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), phosphate buffer (PB), and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma Aldrich (St. Louis, MO, USA). Rabbit anti-PSD-95 and goat anti-NQO1 were purchased from Abcam (Cambridge, UK). Rabbit anti-HO-1 was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Rabbit anti-phosphorylated CREB (pCREB) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-NeuN was purchased from Millipore (Billerica, MA, USA). Normal goat serum (NGS), avidin-viotin complex (ABC), and biotinylated anti-mouse and anti-rabbit antibodies were purchased from Vector Lab (Burlingame, CA, USA).
Kim N., Choi J.G., Park S., Lee J.K, & Oh M.S. (2018). Butterbur Leaves Attenuate Memory Impairment and Neuronal Cell Damage in Amyloid Beta-Induced Alzheimer’s Disease Models. International Journal of Molecular Sciences, 19(6), 1644.
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3

Endothelial Cell Signaling Pathways

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Recombinant human VEGF-A was purchased from R&D Systems (Minneapolis, USA). We obtained the mouse anti-phospho-ERK1/2 (Y204) and anti-GAPDH from Santa Cruz Biotechnology (Heidelberg, Germany). The anti-phospho-VEGFR-2 (Y1175), anti-phospho-Src (Y419) and anti-phospho-FAK (Y397) were obtained from Abcam (Cambridge, UK). The rabbit anti-HO-1 was purchased from Enzo Life Sciences (Exeter, UK). All antibodies used in the study are summarised in Table 1. Basement membrane matrix was from BD Biosciences (Oxford, UK). All other cell culture reagents and chemicals, including dimanganese decacarbonyl Mn2CO10 (CORM-1), tricarbonyldichlororuthenium (II) dimer [Ru(CO)3Cl2]2 (CORM-2), tricarbonylchloro(glycinate)-ruthenium (II) Ru(CO)3Cl-glycinate (CORM-3) and sodium boranocarbonate Na2(H3BCO2) (CORM-A1) were obtained from Sigma-Aldrich (Dorset, UK), unless otherwise stated.
Kourti M., Westwell A., Jiang W, & Cai J. (2019). Repurposing old carbon monoxide-releasing molecules towards the anti-angiogenic therapy of triple-negative breast cancer. Oncotarget, 10(10), 1132-1148.
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4

Western Blot Analysis of Iron Metabolism Proteins

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Protein extracts were prepared with cytoplasmic lysis buffer (25mM Tris-HCl [pH7.4], 40mM KCl, and 1% Triton X-100) supplemented with 1 mg/ml aprotinin and 1 mg/ml leupeptin (all obtained from Sigma). Twenty micrograms of total protein was run on 10%−15% SDS-polyacrylamide gels, and Western blotting was performed exactly as described (Theurl et al., 2006 (link)) with a mouse anti-human TfR1 antibody (1:1000; Invitrogen), rabbit anti-human Ferritin (1:500; Sigma), rabbit anti-Fpn (1:400; self designed; Eurogentic), rabbit anti-HO-1 (1:1000; Enzo), rabbit anti-iNOS (1:1000; BD), rabbit anti-NFκB p65 or rabbit anti-phosphor NFκB p65 (both 1:1000; Cell Signalling). Blotting with either rabbit anti-TBP (1:1000; Cell Signalling) or rabbit anti-Actin antibody (1:1000; Sigma-Aldrich) was performed as a loading control.
Mitterstiller A.M., Haschka D., Dichtl S., Nairz M., Demetz E., Talasz H., Soares M.P., Einwallner E., Esterbauer H., Fang F.C., Geley S, & Weiss G. (2016). Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection. Cellular microbiology, 18(10), 1374-1389.
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5

Neuroprotective Mechanisms of Dimethyl Fumarate

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Dimethyl fumarate (DMF) and mono-methyl fumarate (MMF) were purchased from Sigma-Aldrich. Poly-d-lysine, DMSO and Glutamax were purchased from Sigma. HBSS, DMEM, fetal calf serum, Neurobasal medium, B-27 supplement were purchased from Invitrogen. LIVE/DEAD viability/cytotoxicity kit was purchased from Molecular Probes. The cytotoxicity detection kit plus (lactate dehydrogenase (LDH)) was purchased from Roche. The following primary antibodies were used in these experiments: rabbit anti-Nrf2 (1:200, Santa Cruz Biotechnology), rabbit anti-HO-1 (1:4000, Enzo Life Sciences), rabbit anti-β-tubulin (1:1000, Cell Signaling Technology), mouse anti-rat CD-3 (1:25, BD Biosciences), rabbit anti-myeloperoxidase (MPO) (1:300, DAKO), mouse anti-CD-68 (1:100, Abcam), and rabbit anti-iNOS (1:80, Abcam).
Lin R., Cai J., Kostuk E.W., Rosenwasser R, & Iacovitti L. (2016). Fumarate modulates the immune/inflammatory response and rescues nerve cells and neurological function after stroke in rats. Journal of Neuroinflammation, 13, 269.
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6

Fluorescent Imaging of Mycobacterium tuberculosis

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Mtb expressing mCherry was grown in 7H9 media to log phase and then
fixed in 4% paraformaldehyde for 30 minutes. Fixed Mtb was then
incubated with primary antibodies against Mtb (rabbit anti-Mtb), HO1 (rabbit
anti-HO1; Enzo Life Sciences) or rabbit IgG all at a 1:100 dilution. Then, an
Alexa 488 conjugated donkey anti-rabbit secondary (1:500, A21206, Life
Technologies) was added. After incubation, Mtb were immobilized on glass slides
and imaged using a Zeiss Axioplan 2 fluorescence microscope.
Scharn C.R., Collins A.C., Nair V.R., Stamm C.E., Marciano D.K., Graviss E.A, & Shiloh M.U. (2016). Heme oxygenase-1 regulates inflammation and mycobacterial survival in human macrophages during M. tuberculosis infection. Journal of immunology (Baltimore, Md. : 1950), 196(11), 4641-4649.
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7

Angiogenesis Pathway Protein Analysis

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Recombinant human VEGF-A was purchased from R&D Systems (Minneapolis, USA).
We obtained the mouse anti-phospho-ERK1/2 (Y204) and anti-GAPDH from Santa Cruz Biotechnology (Heidelberg, Germany). The anti-phospho-VEGFR-2 (Y1175), antiphospho-Src (Y419) and anti-phospho-FAK (Y397) were obtained from Abcam (Cambridge, UK). The rabbit anti-HO-1 was purchased from Enzo Life Sciences (Exeter, UK). All antibodies used in the study are summarised in Table 1. Basement membrane matrix was from BD Biosciences (Oxford, UK). All other cell culture reagents were obtained from Sigma-Aldrich (Dorset, UK), unless otherwise stated.
Kourti M., Cai J., Jiang W, & Westwell A.D. (2021). Structural Modifications on CORM-3 Lead to Enhanced Anti-angiogenic Properties Against Triple-negative Breast Cancer Cells. Medicinal chemistry (Shariqah (United Arab Emirates)), 17(1).
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8

Western Blot Analysis of Antioxidant Proteins

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Western blot analysis was performed as described previously [28 (link)] using the following antibodies: mouse anti-β-Actin (Sigma-Aldrich), rabbit anti-Nrf2 (Abcam, Cambridge, United Kingdom), rabbit anti-KEAP1 (Proteintech Group, Rosemont, IL, USA), rabbit anti-HO-1 (Enzo Life Science, Lörrach, Germany) and rabbit anti-GPX4 (R&D Systems, Minneapolis, MN, USA). Goat anti-mouse IgG and goat anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies. Enhanced chemiluminescence was used for detection (Amershan Bioscience, Freiburg, Germany). Representative blots of at least two independent experiments are shown.
Lippmann J., Petri K., Fulda S, & Liese J. (2020). Redox Modulation and Induction of Ferroptosis as a New Therapeutic Strategy in Hepatocellular Carcinoma. Translational Oncology, 13(8), 100785.
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