Bx51 microscope

Manufactured by Olympus

Sourced in Japan, United States, Germany, Italy, Denmark, United Kingdom, Canada, France, China, Australia, Austria, Portugal, Belgium, Panama, Spain, Switzerland, Sweden, Poland

The BX51 microscope is an optical microscope designed for a variety of laboratory applications. It features a modular design and offers various illumination and observation methods to accommodate different sample types and research needs.

Automatically generated - may contain errors

Lab products found in correlation

3 784 protocols using bx51 microscope

Simm530 Modulates Cell Migration and Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the migration assay, NCI-H441 cells suspended in serum-free medium (1.5×10⁵ cells per well) were seeded in 24-well Transwell plates (pore size, 8 μm; Corning). The bottom chambers were filled with serum-free medium supplemented with HGF (100 ng/mL), and 0.8, 4, 20 and 100 nM of Simm530 was added to both sides of the membrane. The cultures were maintained for 24 h, followed by the removal of non-motile cells at the top of the filter using a cotton swab. The migrating cells were fixed in paraformaldehyde (4%) and stained with crystal violet (0.1%) for 15 min at room temperature. The dye that was taken up by the cells bound to the membrane was released by the addition of 100 μL 10% acetic acid, and the absorbance of the resulting solution was measured at 595 nm using a multiwell spectrophotometer (SpetraMAX 190, from Molecular Devices, Palo Alto, CA, USA). The assay was performed in triplicate. Images were obtained using an Olympus BX51 microscope.
For the invasion assay, NCI-H441 cells were cultured in the top chambers containing Matrigel-coated membrane inserts (Matrigel, BD). The ensuing procedure was identical to the migration assay. The assay was performed in triplicate. Images were obtained using an Olympus BX51 microscope.

Wang Y., Zhan Z., Jiang X., Peng X., Shen Y., Chen F., Ji Y., Liu W., Shi Y., Duan W., Ding J., Ai J, & Geng M. (2016). Simm530, a novel and highly selective c-Met inhibitor, blocks c-Met-stimulated signaling and neoplastic activities. Oncotarget, 7(25), 38091-38104.

+ Open protocol

+ Expand

Histological analysis of Pst infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

For histological observations, the leaves inoculated with Pst were sampled. For histological observations of fungal growth, wheat germ agglutinin (WGA) conjugated to the fluorophore Alexa‐488 (Invitrogen) was used to stain Pst infection structures. The stained Pst infection structures were observed with an Olympus BX‐51 microscope (Olympus) under blue‐light excitation (excitation wavelength 450–480 nm, emission wavelength 515 nm). For histological observations of host responses, H₂O₂ production in infected wheat leaves was detected by staining with 3,3′‐diaminobenzidine (DAB; Amresco) as described previously (Wang et al., 2007 ), and observed with a BX‐51 microscope (Olympus) under differential interference contrast optics. The number of haustorial mother cells and haustoria, hyphal length, infection area, and H₂O₂ area per infection site were measured using the cellSens Entry software (Olympus).

He F., Wang C., Sun H., Tian S., Zhao G., Liu C., Wan C., Guo J., Huang X., Zhan G., Yu X., Kang Z, & Guo J. (2022). Simultaneous editing of three homoeologues of TaCIPK14 confers broad‐spectrum resistance to stripe rust in wheat. Plant Biotechnology Journal, 21(2), 354-368.

+ Open protocol

+ Expand

Stereological Quantification of PV and SOM Cells in Nlgn3 Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unbiased stereological estimation of total PV and SOM cells in the somatosensory cortex of WT and Nlgn3^R451C mice was acquired using the optical fractionator approach and the Stereo Investigator software (MicroBrightField, Inc.) equipped with an Olympus BX51 microscope. Boundaries of the entire somatosensory cortex were determined using anatomical landmarks, the ALLEN Mouse Brain Reference Atlas Version 2 (2011), and Paxinos and Franklin Mouse Brain Atlas (Second Edition) and traced at low magnification (2x, NA 0.05). A sampling grid of 700 μm x 700 μm, counting frame of 100 μm x 100 μm, and dissector height of 10 μm (with 1–3 μm guard zones) were used to estimate the number of positively stained cells in a 1:10 series for each animal at high magnification (40x, NA 0.75). The Gundersen coefficient of error (m = 0) was below 0.1 for all animals (n = 10 for both WT and Nlgn3^R451C groups) (Gundersen and Jensen, 1987). Data are shown as % of the WT average cell number for either PV neurons or SOM neurons in Nlgn3^R451C mice (mean ± SEM). Representative images were taken using the 40x objective on the same Olympus BX51 microscope used for stereology.

Speed H.E., Masiulis I., Gibson J.R, & Powell C.M. (2015). Increased Cortical Inhibition in Autism-Linked Neuroligin-3R451C Mice Is Due in Part to Loss of Endocannabinoid Signaling. PLoS ONE, 10(10), e0140638.

+ Open protocol

+ Expand

Lung Histology Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All histologic analysis were performed as described before [15 (link)]. In brief, lungs were fixed by instillation of a buffered 4% formaldehyde solution under a constant hydrostatic pressure (30 cm H₂O for 15 minutes), kept in 4% formaldehyde solution for additional 24 hours without hydrostatic pressure, embedded in 1% agarose, cut into slices of exactly the same thickness (5 mm), and embedded in paraffin. Primary antibodies for CD3 (rabbit, 1/100, ab5690; Abcam, Cambridge, UK) and Ly6B (rat, 1/150, MCA771GA; Bio-Rad, Munich, Germany) were used for immunohistochemistry analysis blinded to the investigator. Corresponding HRP-conjugated secondary antibodies (anti-rabbit 414341F and anti-rat 414311F, Histofine Simple Stain, Nichirei Biosciences Inc. Japan) were used. Randomly selected fields were evaluated for positive cells using the Visiopharm Integrator System (Visiopharm, Hoersholm, Denmark) on an Olympus BX51 microscope. Paraffin sections were stained with hematoxylin-eosin (H&E), blinded to the investigator and the inflammatory score was calculated as described before [6 (link), 16 (link)]. The mean chord length (MCL) was calculated blinded to the investigator using the Visiopharm Integrator System on an Olympus BX51 microscope equipped with an 8-position slide loader.

Vella G., Ritzmann F., Wolf L., Kamyschnikov A., Stodden H., Herr C., Slevogt H., Bals R, & Beisswenger C. (2021). IL-17C contributes to NTHi-induced inflammation and lung damage in experimental COPD and is present in sputum during acute exacerbations. PLoS ONE, 16(1), e0243484.

+ Open protocol

+ Expand

Muscle Fiber and Capillary Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the euthanasia, the vastus medialis was excised by transecting the muscle origins and insertions. Muscles were placed on a mounting block and frozen in liquid nitrogen–cooled isopentane (2‐methylbutane). Samples were sectioned at 5 μm at −20°C. Cross‐cryosections were stained with NADH‐tetrazolium reductase to examine the oxidative capacity of muscle fibers. Based on their NADH content, fibers were classified as type I (oxidative), type IIA (aerobic glycolytic), and type IIB (anaerobic glycolytic). Fiber types of the whole muscle section were counted using an Olympus BX51 microscope connected to a movable platform and the computer‐assisted stereological toolbox (CAST) software count tool.
Capillaries were identified with the endothelial‐specific marker CD31. Briefly, muscle sections were incubated with rat anti‐CD31 monoclonal antibody, clone MEC 13.3 (BD Biosciences) and a donkey anti‐rat IgG secondary antibody (Jackson Immunoresearch). CD31‐positive structures of muscle cryosections were counted using an Olympus BX51 microscope connected to a movable platform and the computer‐assisted stereological toolbox (CAST) software count tool.

Mas‐Stachurska A., Siegert A., Batlle M., Gorbenko del Blanco D., Meirelles T., Rubies C., Bonorino F., Serra‐Peinado C., Bijnens B., Baudin J., Sitges M., Mont L., Guasch E, & Egea G. (2017). Cardiovascular Benefits of Moderate Exercise Training in Marfan Syndrome: Insights From an Animal Model. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(9), e006438.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Mouse and Human Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical stains were performed on 40 μm free-floating sections of 4% PFA-fixed mouse brains and 16 μm mounted sections of 4% PFA-fixed human brains prepared using a Leica cryostat. The staining protocol included antigen retrieval treatment by incubating tissue sections in 10 mM sodium citrate (pH 6.0) at 90°C for 10 minutes. Immunostains were developed using DAB and counterstained with Nissl or hematoxylin. Information on primary antibodies is provided in Supplemental Table 5. Images were captured using an Aperio ImageScope (Leica Biosystems) with a 20× objective zoom ×2. GAD1⁺, PV⁺, NGRN⁺, and MAP2⁺ neurons in human tissue were counted using Neurolucida 2017 (MBF Biosciences) on a PC attached to an Olympus BX51 microscope with a 20× objective. IBA1⁺ microglia and GFAP⁺ astrocytes in human and mouse tissues were counted using an optical fractionator–based method using Stereology Investigator 2017 (MBF Biosciences) on a PC attached to an Olympus BX51 microscope with a 60× objective and a motorized XYZ stage.

Marsan E., Velmeshev D., Ramsey A., Patel R.K., Zhang J., Koontz M., Andrews M.G., de Majo M., Mora C., Blumenfeld J., Li A.N., Spina S., Grinberg L.T., Seeley W.W., Miller B.L., Ullian E.M., Krummel M.F., Kriegstein A.R, & Huang E.J. (2023). Astroglial toxicity promotes synaptic degeneration in the thalamocortical circuit in frontotemporal dementia with GRN mutations. The Journal of Clinical Investigation, 133(6), e164919.

+ Open protocol

+ Expand

Juvenile Floral Tissue Histological Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The juvenile flower tissues (0.5–0.8 mm in length) of se1 and V2709 were collected and fixed in the formalin/acetic acid/alcohol (FAA) fixative [formalin:70% ethanol:acetic acid = 18:1:1 (v/v)]. The samples were placed in a vacuum for 30 min and stored in 70% ethanol at room temperature. The samples were then dehydrated, embedded, trimmed, sectioned, stained, and de-waxed as previously described (Jackson et al., 1994 ). Sections were observed under an Olympus BX51 microscope (Olympus, Japan).
For observation of the vasculature pattern, 0.5 cm² of the standard tissue was fixed for 4 h in ethanol/acetic acid (6:1) at room temperature. After bleaching in 100% ethanol overnight, the samples were mounted in a mixture of chloralhydrate/glycerol/water (8:1:2) for 1 h at room temperature. Tissues were observed under an Olympus BX51 microscope (Olympus, Japan).

Lin Y., Laosatit K., Chen J., Yuan X., Wu R., Amkul K., Chen X, & Somta P. (2020). Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata). Frontiers in Plant Science, 11, 575922.

+ Open protocol

+ Expand

Formaldehyde Fixation and Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells cultured for up to 72 h were washed with PBS and fixed in 4% formaldehyde for 10 min at room temperature. For nuclei analysis, cells were stained with Hoechst 33258 and examined by using the Olympus BX51 microscope. For morphological analysis, cells were stained with haematoxylin for 1 min and washed again before staining with eosin for 30 seconds. Slides were mounted with 50% glycerol, sealed and observed under Olympus BX51microscope.

Morelli M.B., Amantini C., Nabissi M., Cardinali C., Santoni M., Bernardini G., Santoni A, & Santoni G. (2016). Axitinib induces senescence-associated cell death and necrosis in glioma cell lines: The proteasome inhibitor, bortezomib, potentiates axitinib-induced cytotoxicity in a p21(Waf/Cip1) dependent manner. Oncotarget, 8(2), 3380-3395.

+ Open protocol

+ Expand

Assessing Cell Migration and Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ater cultured 2 days; SGC-7901/DDP cells (5 × 10 5 /ml cells/well) were scratched with serum deprived in 24-well plate. 24 h later, cells were xed with methanol, stained with crystal violet, and viewed under an Olympus BX-51 microscope. Transwell assay After coating with matrigel, SGC-7901/DDP cells were placed into the upper chamber (8 μm pore size; Millipore, USA) in 1% FBS media. 48h later, cells were stained with methanol and 0.1% crystal violet, and viewed under an Olympus BX-51 microscope.

Chen H., Liu P., Wang L., Yu Y., Zhao Y., Cai Y., Wang Z., & Zhang M. (2020). MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer.

+ Open protocol

+ Expand

Staining and Imaging Diplozoid Parasites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Diplozoid parasites preserved in 70% ethanol were stained with acetic carmine, differentiated using HCl in 30% ethanol, dehydrated in graded ethanol series (50%, 70%, 80%, 90%, 95% and 100%), cleared in clove oil, and mounted in Canada balsam [17 ]. An Olympus BX51 microscope (Olympus, Tokyo, Japan) was used to examine and photograph the specimens. The illustrations were created using an Olympus BX51 microscope’ drawing apparatus and then processed on a computer using Photoshop CS4.0 (Adobe, San Jose, CA, USA). Olympus DP22 software was used to take measurements. Measurements are in micrometres (mm) and are shown as the mean followed by the range and the number of measured specimens in parentheses. The haptoral terminology used herein follows Pečínková et al. [38 (link)].

Huang J., Zhou X., Yuan K, & Ding X. (2023). Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae), a gill parasite of Cirrhinus molitorella (Cyprinidae, Labeoninae) in South China. Parasite, 30, 20.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!