Rabbit anti phospho irf3

Rabbit anti-phospho-IRF3 (Cat# 4947, 1:1000), rabbit anti-IRF3 (Cat# 4302, 1:1000), rabbit anti-phospho-TBK1 (Cat# 5483, 1:1000) and rabbit anti-cGAS antibodies (Cat# 15102, Cat# 79978, Cat# 31659 (Mouse specific), 1:1000) were from Cell Signaling Technology. Rabbit anti-TBK1 (Cat# ab40676, 1:1000) was from Abcam. Rabbit anti-PCBP2 (Cat# 15070-1-AP, 1:1000) was from Proteintech. Mouse anti-PCBP2 (Cat# sc-101136, 1:1000) was from Santa Cruz Biotechnology. Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# KM9002, 1:4000), mouse anti-α-Tubulin (Cat# KM9007, 1:8000), and mouse anti-HA (Cat# KM8004, 1:1000) antibodies were from Sungene Biotechnology. Rabbit anti-Flag (Cat# F7425, 1:1000) antibody was from Sigma. Mouse anti-Flag (Cat# M185-3, 1:1000), rabbit anti-HA (Cat# M132-3, 1:1000), rabbit anti-Myc (Cat# 562, 1:1000), mouse anti-Myc (Cat# M192-3, 1:1000) antibodies were from MBL. Mouse anti-V5 antibody (Cat# YM3005, 1:1000) was from ImmunoWay Biotechnology Company. Rabbit anti-PCBP1 (Cat# A1044, 1:1000) antibody was from ABclonal Technology. Mouse/Rabbit anti-cGAS antibody was prepared in our laboratory, the cGAS antibody was generated by immunizing mice or rabbits with purified human cGAS full-length from E. coli.

Antibodies used for immunoblot and immunofluorescence analysis include: rabbit anti-Flag (1:1000, Sigma), mouse anti-Flag M2 (1:1000, Sigma), rabbit anti-IRF3 (1:100 or 1:500, Cell Signaling Technology), mouse anti-IRF3 (1:1000, Cell Signaling Technology) rabbit anti-TBK1 (1:1000, Cell Signaling Technology), rabbit anti-phospho-TBK1 (1:1000, Cell Signaling Technology), rabbit anti-phospho-IRF3 (1:1000, Cell Signaling Technology), rabbit anti-IFIT1 (1:1000, Cell Signaling Technology), human anti-SARS-CoV-2 Spike (CR3022, 1:5000), rabbit anti-SARS-CoV-2 nucleocapsid (Genetex, 1:1000), rabbit anti-NF-κB (1:500, Cell Signaling Technology), rabbit anti-phospho-NF-κB (1:1000, Cell Signaling Technology), mouse anti-Strep (1:1000, Biolegend; 1:1000, Genscript), rabbit anti-Actin (1:1000, Cell Signaling Technology), Streptavidin-HRP (1:1000, Jackson ImmunoResearch), species-specific HRP-Conjugated antibodies (1:10,000, Jackson ImmunoResearch). Alexa Fluor conjugated secondary antibodies (1:500, Life Technologies), and nuclear counterstain 4’,6’-Diamidino-2-phenylindole dihydrochloride- (DAPI; 1:1000, ACROS Organics).

All common chemicals were obtained from Sigma-Aldrich (Wicklow, Ireland) unless otherwise stated. Antibodies used for Western blot and co-immunoprecipitation include rabbit anti-Bid (Abcam, ab62469, 1:1000), rabbit anti-Peli1 (Abcam ab199336, 1:1000), rabbit anti-TRAF3 (Abcam, ab76147, 1:500), mouse anti-TRAF6 (Santa Cruz, sc8409, 1:200), rabbit anti-phospho-IRF3 (Cell Signaling, 4947P, 1:500), rabbit anti-phospho-TBK1 (Cell Signaling, 5483P, 1:500), rabbit anti-phospho c-Jun (Cell Signaling, 9261S, 1:1000), rabbit anti-A20/TNFAIP3 (Cell Signaling, 5630, 1:1000), rabbit anti-LC3 (Abcam, ab51520, 1:2000), rabbit anti-FLAG (OctA-Probe D-8, Santa Cruz, sc-807, 1:500), anti- α-Tubulin (Sigma-Aldrich, T6199, 1:5000), anti-β-Actin (Sigma-Aldrich, A3853, 1:5000), and anti-GAPDH (Abcam, ab8245-100,1:5000). Bortezomib was obtained from Millennium Pharmaceuticals (Cambridge, MA, USA). Lipopolysaccharide (LPS) was obtained from (Sigma-Aldrich, L4391), and PolyI:C (31852-29-6) and Pam₃CSK4 (112208-00-1) were obtained from InvivoGen.