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In vivo ms fx pro imaging system

Manufactured by Bruker
Sourced in United States

The In Vivo MS FX pro Imaging System is a versatile and powerful mass spectrometry-based imaging platform designed for in vivo applications. It enables high-resolution spatial mapping of a wide range of analytes, including metabolites, lipids, and proteins, within small animal models. The system combines advanced mass spectrometry technology with state-of-the-art imaging capabilities to provide researchers with a comprehensive tool for studying biological processes in a non-invasive manner.

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4 protocols using in vivo ms fx pro imaging system

1

Intracranial Tumor Growth Monitoring

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BALB/c nude mice were maintained and bred in a specific-pathogen free (SPF) environment, adhering to standard conditions of temperature (20–26 °C) and humidity (40–70%). They were subjected to a strict 12-h light cycle, with lights on at 08:00 a.m. and off at 08:00 p.m. No limitations were imposed on the sex of the experimental animals involved in this study. Cells expressing firefly luciferase mixed with Matrigel (Corning, NY, USA) were intracranially injected into 5- to 6-week-old female athymic nude mice. The intracranial injection point was at the cerebral cortex, 1 mm prior to coronal suture, 1 mm on the right side of the centreline, and 3 mm below the dura mater. Appropriate medications were provided to reduce pain. To monitor intracranial tumour growth, the animals were intraperitoneally injected with D-luciferin (Yeasen, #40902ES01) and anaesthetized with isoflurane. The images were captured using an In Vivo MS FX pro Imaging System (Bruker, MA, USA) or IVIS Lumina imaging station (Perkin Elmer, MA, USA). The results were reported as the total flux (photons/second). The mice were sacrificed at the indicated time points. The brains were removed, fixed in 4% paraformaldehyde, and embedded in paraffin.
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2

Xenograft Mouse Model for Cancer

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All experiments were performed in accordance with the relevant guidelines and regulations that were approved by the Institutional Animal Care and Use Committee at Yale University. Six-week-old female athymic nude mice were purchased from Harlan Sprague Dawley Inc. (Indianapolis, IN). The animals were maintained under pathogen-free conditions, and food and water were supplied ad libitum. Mice were ear-tagged and followed individually throughout the study. Mice were anesthetized by gas anesthesia (3% isoflurane) prior to injection or imaging. In the interperitoneal injection model, 7 × 106 cells (in 300 μl of RPMI-1640 medium) were injected into mice. In the intrauterine and injection model, 2 × 106 cells (in 50 μl of RPMI-1640 medium) were injected into one side uterine horn by surgery. Superovulation was induced by interperitoneally injecting 5 IU PMS and 48 hours later 5 IU HCG. Cancer cells were injected 24 hours after PMS injection. Tumors were monitored by red fluorescence and, concurrently, X-ray images with the Bruker In-Vivo MS FX PRO Imaging System (Billerica, MA).
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3

Biodistribution of Pcbp2 siRNA Nanocomplex

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The biodistribution study was performed in a rat model of liver fibrosis as described previously [13 (link)]. The animal protocol (protocol number: 1110) was approved by the University of Missouri-Kansas City (UMKC) Institutional Animal Care and Use Committee (IACUC). Six male Sprague Dawley rats were intraperitoneally injected with a mixture of CCl4 and olive oil (1:1, v/v) at a dose of 1 mL/kg CCl4 twice a week for 4 weeks. The rats were then divided into two groups and intravenously administered with a free Cy5-labeled Pcbp2 siRNA and a dimeric IGF2R-modified Cy5-labeled siRNA-loaded nanocomplex at a dose of 1 mg/kg siRNA. After 2 h, the rats were euthanized, and their organs, including the liver, lungs, spleen, kidneys, heart, and blood, were harvested for image analysis using a Bruker MS FX PRO In Vivo Imaging System (Billerica, MA, USA). The fluorescence intensity in the region of interest (ROI) was determined using Bruker molecular imaging software.
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4

Tracking Liver Fibrosis Progression

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The animal protocol was approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Missouri-Kansas City. Male Sprague Dawley rats were housed in a humidity and temperature controlled room with a 12 h light–dark cycle. Liver fibrosis was induced by intraperitoneal injection with the mixture of carbon tetrachloride (CCl4) and olive oil (1:1, v/v) at a dose of 1 mL/kg CCl4 twice a week for five consecutive weeks. The rats were then randomly divided into four groups and intravenously injected with Cy5-labeled siRNA or the siRNA nanocomplexes encapsulating Cy5-labeled siRNA at a dose of 0.1 mg/Kg. After 2 h, the rats were euthanized, and major organs including the liver, lungs, spleen, kidneys, heart, muscle (thigh), and blood were harvested for imaging analysis using a Bruker MS FX PRO In vivo Imaging System (Billerica, MA).
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