Rpmi 1640 medium and supplements for cell proliferation

2-Hydroxyethyl methacrylate (H), gelatin (G, Type A), ethylene glycol dimethacrylate (EGDMA), 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), ammonium persulfate (APS), and N,N,N’,N’-tetramethylene diamine (TEMED) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Manuka honey (MHo) was supplied by Haines Apiaries Ltd. (ArtisanHoney^®, Kaitaia, New Zealand). RPMI-1640 medium and supplements for cell proliferation, as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components, were purchased from Sigma-Aldrich, St. Louis, MO, USA. All syntheses were performed in deionized water. All experiments were performed using lab-produced, ultra-distilled water.

2-Hydroxyethyl methacrylate monomer (HEMA), gelatin from porcine skin (G, Type B), sodium alginate (A), ethyleneglycol dimethacrylate (EGDMA), as well as crosslinker 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), potassium persulfate (PPS), N,N,N′,N′-tetramethylene diamine (TEMED), Pluronic F-172, sodium bicarbonate (NaHCO₃) were purchased from Sigma-Aldrich, St. Louis, MO, USA. RPMI-1640 medium and supplements for cell proliferation as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich. All syntheses were performed in deionized water. Materials used for the hydroxyapatite synthesis and doping were calcium nitrate pentahydrate (Ca(NO₃)₂×5H₂O) (Sigma-Aldrich), magnesium nitrate hexahydrate (Mg(NO₃)₂×6H₂O), strontium nitrate (Sr(NO₃)₂), gallium nitrate hydrate (Ga(NO₃)₃×H₂O), zinc nitrate hexahydrate (Zn(NO₃)₂×6H₂O), ammonium dihydrogen phosphate (NH₄H₂PO₄) were obtained from Sigma-Aldrich, and urea ((NH₂)₂CO) was purchased from Alfa Aesar (Haverhill, MA, USA), and all were of analytical grade. All experiments were performed using lab-produced, ultra-distilled water.

The monomer 2-hydroxyethyl methacrylate (HEMA, 99%) and polymers: gelatin from porcine skin (G, Type A) and sodium alginate (A) and nanomaterial graphene oxide (GO, nanocolloids, dispersion in water) were supplied from Sigma-Aldrich, Germany. Cross-linking agents: N,N′-methylenebis(acrylamide) (BIS) and glutaraldehyde, initiator-ammonium persulfate (APS), activator-N,N,N′,N′-tetramethylene diamine (TEMED), foaming stabilizer-Pluronic F-172 were obtained from Sigma-Aldrich, Germany. Foaming agents-sodium bicarbonate (NaHCO₃) and curcumin (from Curcuma longa) were purchased from Sigma-Aldrich, Germany. RPMI-1640 medium and supplements for cell proliferation, as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich. Distilled water was used for all polymerizations and preparations of buffer solution.