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Sodium phosphate monobasic

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Sodium phosphate monobasic is a chemical compound commonly used as a lab reagent. It is a white, crystalline solid that is soluble in water. The primary function of sodium phosphate monobasic is to act as a pH buffer in various laboratory applications, helping to maintain a specific pH level in solutions.

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Lab products found in correlation

Sodium phosphate dibasic, by Merck Group (166 mentions) Sodium chloride (nacl), by Merck Group (69 mentions) Sodium hydroxide (naoh), by Merck Group (50 mentions) Hydrochloric acid (hcl), by Merck Group (31 mentions) Methanol, by Merck Group (31 mentions) Acetic acid, by Merck Group (29 mentions) Potassium chloride (kcl), by Merck Group (24 mentions) Acetonitrile, by Merck Group (24 mentions) Bovine serum albumin (bsa), by Merck Group (20 mentions) Ethanol, by Merck Group (19 mentions) Sodium carbonate, by Merck Group (18 mentions) Dimethyl sulfoxide (dmso), by Merck Group (17 mentions) Formic acid, by Merck Group (16 mentions) Sodium acetate, by Merck Group (15 mentions) Sodium bicarbonate, by Merck Group (13 mentions) Sodium azide, by Merck Group (13 mentions) Milli q system, by Merck Group (13 mentions) Calcium chloride, by Merck Group (12 mentions) D glucose, by Merck Group (12 mentions) Ascorbic acid, by Merck Group (12 mentions)

289 protocols using sodium phosphate monobasic

1

Synthesis of [11C]Methyl Iodide from [11C]CO2

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The methionine sulfoxide, methionine sulfone, methylene iodide and sodium phosphate monobasic were purchased from Sigma-Aldrich (San Luis, Missouri). For the preparation of the QC HPLC solvent and the phosphate buffer used to elute the reaction mixture of the cartridge, sodium phosphate monobasic was diluted with water to the specified concentration, without adjusting the pH. The sodium hydroxide was purchased from Merck (Darmstadt, Germany). The saline injection (NaCl 0.9%) was supplied by Fármaco Uruguayo Laboratory (Montevideo, Uruguay). Sep-Pak Light C18 and Sep-Pak tC18 Plus Short were obtained from Waters (Milford, MA).
[11C]CO2 was produced by the 16.5 MeV proton bombardment of a N2/O2 (1% O2 in N2) target using an GE PETtrace 800 Series Cyclotron (GE Healthcare, Chicago, Illinois). The [11C] CO2 was converted to [11C] methyl iodide in the gas phase using a GE TRACER FX C Pro module (GE Healthcare, Chicago, Illinois).
Radioactivity was measured using a Capintec (Ramsey, NJ) CRC-25 PET dose calibrator.
Giglio J., Rosas G., Basso M., Boné A., Savio E, & Engler H. (2018). An alternative methodology for the determination of the radiochemical purity of 11C-methionine. EJNMMI Radiopharmacy and Chemistry, 3, 15.
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2

Resorcinol Spectrophotometric pH Analysis

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All chemical reagents were of analytical grades and used as received without further purification: resorcinol (1,3-dihydroxybenzene, 99%, Sigma-Aldrich), hydrochloric acid (HCl, 37%, Sigma-Aldrich), sodium hydroxide (NaOH, ≥97.0%, Sigma-Aldrich), sodium phosphate dibasic (Na2HPO4, ≥99.0%, Sigma-Aldrich), sodium phosphate monobasic (NaH2PO4, ≥99.0%, Sigma-Aldrich), and potassium chloride (KCl, ≥99.0%, Sigma-Aldrich).
Buffer solutions were freshly prepared as follows: pH 1.0 – hydrochloric acid (HCl), pH 4.0 – citric acid/sodium citrate, pH 6.0 – sodium phosphate monobasic (NaH2PO4)/sodium phosphate dibasic (Na2HPO4), pH 9.0 – sodium carbonate (Na2CO3)/sodium bicarbonate (NaHCO3), pH 10.0 – sodium bicarbonate (NaHCO3) and sodium hydroxide (NaOH), pH 12.0, 13.0, 14.0 – sodium hydroxide (NaOH). The ionic strengths of all buffer solutions were adjusted to 0.10 M by the addition of potassium chloride (KCl).
Ngamchuea K., Tharat B., Hirunsit P, & Suthirakun S. (2020). Electrochemical oxidation of resorcinol: mechanistic insights from experimental and computational studies. RSC Advances, 10(47), 28454-28463.
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3

Biochemical Reagents and Purified Proteins

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Sodium chloride, sodium hydroxide, sodium
phosphate monobasic of p.a. quality, and o-phosphoric
acid (85%, HPLC) were purchased from Merck (Darmstadt, Germany). Hydrochloric
acid and Tris–HCl of p.a. quality were purchased from Honeywell
(Morris Plains, NJ, USA) and Kemika (Zagreb, Croatia), respectively.
The deionized water was purified with a Milli-Q purification system
from Millipore (Bedford, MA, USA) before use.
β-lactoglobulin
of ≥90% purity, lysozyme from chicken egg white of ≥98%
purity, and bovine serum IgG of ≥95% purity, all in the form
of lyophilized powder, were purchased from Sigma-Aldrich.
Kristl A., Caf M., Pompe M, & Podgornik A. (2022). Complex Protein Retention Shifts with a Pressure Increase: An Indication of a Standard Partial Molar Volume Increase during Adsorption?. Analytical Chemistry, 94(39), 13350-13358.
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4

Laccase-Catalyzed Catechol Oxidation

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Catechol, potassium chloride (KCl), sodium acetate (AcONa), sodium phosphate dibasic, sodium phosphate monobasic, glutaraldehyde 25% v/v, and laccase from Trametes versicolor (TvLac) were purchased from Merck Life Science (Milan, Italy). All solutions were prepared using Milli-Q water (R = 18.2 MΩ cm at 25 °C; TOC < 10 μg L−1, Millipore, Molsheim, France). TvLac was solubilized in sodium acetate buffer (AcONa) 0.01 M, pH 5, stored at −20 °C, and Catechol standard solutions were freshly prepared in AcONa buffer 0.01 M, pH 5, for each experiment.
D’Agostino C., Chillocci C., Polli F., Surace L., Simonetti F., Agostini M., Brutti S., Mazzei F., Favero G, & Zumpano R. (2023). Smartphone-Based Electrochemical Biosensor for On-Site Nutritional Quality Assessment of Coffee Blends. Molecules, 28(14), 5425.
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5

Preparation of Biochemical Reagents

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Sodium phosphate monobasic, sodium phosphate dibasic and sodium chloride were procured from Merck (Japan). Sodium metabisulfite was purchased from Fisher Scientific (UK). Hydrochloric acid was obtained from Merck (Austria). Bovine serum albumin and Bradford reagent were procured from Sigma-Aldrich (USA).
Ibrahim A., Bakar K., Bakar J., Nirmal N.P., Ikhwanuddin M, & Karim N.U. (2023). Effects of Annona muricata Extract on Trypsin, Cathepsin B and Collagenase Activities and Textural Changes in Chilled Macrobrachium rosenbergii. Foods, 12(9), 1887.
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6

Peptide Synthesis and Functionalization

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APTES,
dibenzocyclooctyl-N-hydroxy succinimide (DBCO-NHS), N-(dimethylaminopropyl)-N′-carbodiimide
hydrochloride (EDC), triisopropylsilyl
chloride, and 2-azidoacetic acid were procured from Sigma-Aldrich.
Ethanol (ACS grade), potassium hexacyanoferrate(III), potassium hexacyanoferrate(II),
sodium phosphate dibasic, and sodium phosphate monobasic were purchased
from Merck Chemicals. All chemicals were of analytical reagent (AR)
grade and used without further purification. Aqueous solutions were
prepared using Millipore water received from Milli-Q system (Millipore
Inc.). 9-Fluorenylmethyloxycarbonyl (Fmoc)-protected amino acids (Fmoc-Gln(Trt)-OH,
Fmoc-Asn(Trt)-OH, Fmoc-Gly-OH, Fmoc-Pro-OH, Fmoc-Leu-OH, and Fmoc-Ile-OH)
and Fmoc-Rink-Amide-MBHA resin were procured from Iris Biotech GMPH.
Fmoc-Cys(Trt)-OH and Fmoc-Tyr(tBu)-OH were purchased
from GL Biochem Ltd.
Tadi K.K., Alshanski I., Mervinetsky E., Marx G., Petrou P., Dimitrios K.M., Gilon C., Hurevich M, & Yitzchaik S. (2017). Oxytocin-Monolayer-Based Impedimetric Biosensor for Zinc and Copper Ions. ACS Omega, 2(12), 8770-8778.
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7

Enzymatic Synthesis of Fatty Acid Esters

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Polylactic acid (PLA, Mw = 68 kDa), polyvinylpyrrolidone (PVP, Mw = 360 kDa), lipase from Candida rugosa (CrL), lipase from Rhizomucor miehei (RmL), palmitic acid (PA), oleic acid (OA), linoleic acid (LA), hydrochloride acid (HCl 37%, w/w), methanol, toluene, hexane, ethanol, sodium phosphate monobasic, deuterated chloroform (CDCl3), dichloromethane (DCM), dimethylformamide (DMF), methyl heptadecanoate were purchased from Merck (Darmstadt, Germany). Nadifloxacin was obtained from TCI (Rockenberg, Germany). In all cases, the water was purified by a Millipore Milli Q water purification system (Bradford, MA, USA).
Balogh-Weiser D., Molnár A., Tóth G.D., Koplányi G., Szemes J., Decsi B., Katona G., Salamah M., Ender F., Kovács A., Berkó S., Budai-Szűcs M, & Balogh G.T. (2023). Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique. Pharmaceutics, 15(4), 1174.
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8

Electrochemical Analysis of Molecularly Imprinted Polymers

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The morphological analysis of the molecularly imprinted polymers was conducted using scanning electron microscope model JSM 7500F. The functional groups of the molecularly imprinted polymer were evaluated using Bruker brand FTIR equipment. All the electrochemical measurements were performed using the conventional three−electrode system. The three−electrode system consisted of a glassy carbon electrode (d = 3 mm) as the working electrode, Ag/AgCl reference electrode (KCl 3 mol L−1), and an auxiliary platinum wire electrode. All the electrochemical measurements were performed at room temperature. The electrochemical measurements were conducted using AUTO LAB PGSTAT 100 potentiostat–galvanostat controlled by NOVA 2.1.1 software. All the reagents used for the experiments were of analytical grade, and all solutions were prepared in deionized water obtained from Milli−Q Direct−0.3 (Millipore).
Methotrexate (98%), pyrrole (99%), lithium perchlorate (98%), MWCNT (<5% modified with COOH), potassium hexacyanoferrate (II) trihydrate (98%), and potassium ferricyanide (98%) were purchased from Sigma−Aldrich. Sodium phosphate monobasic (98%), sodium phosphate dibasic (98%), and potassium chloride (98%) were acquired from Merck.
Jara-Cornejo E., Khan S., Vega-Chacón J., Wong A., da Silva Neres L.C., Picasso G, & Sotomayor M.D. (2023). Biomimetic Material for Quantification of Methotrexate Using Sensor Based on Molecularly Imprinted Polypyrrole Film and MWCNT/GCE. Biomimetics, 8(1), 77.
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9

DPPH Radical Scavenging Assay

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2,2-Diphenyl-1-picryl hydrazyl radical (DPPH•), spectrophotometric grade methanol (≥99.9%), and the nonionic detergent Triton X-100 were purchased from Fisher Scientific (Pittsburgh, PA). Citric acid monohydrate (>99% purity) and sodium phosphate monobasic (>98% purity) were purchased from EMD Millipore (San Diego, CA). Acetic acid-d4 (99.5 atom % D), deuterium oxide (99.9 atom % D), and formic acid (~98%) were purchased from Sigma-Aldrich (St. Louis, MO). Doubly distilled water was used throughout the experiments.
Nicklisch S.C., Spahn J.E., Zhou H., Gruian C.M, & Waite J.H. (2016). Redox Capacity of an Extracellular Matrix Protein Associated with Adhesion in Mytilus californianus. Biochemistry, 55(13), 2022-2030.
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10

Enzymatic Assays for Bioactive Compounds

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Gallic acid, quercetin, Folin-Ciocalteu reagent, kojic acid, tyrosinase, acetylcholinesterase (Ache), butyrlcholinesterase (Buche), acetylthiocholine iodide (ATCI), butyrylthiocholine iodide (BUTCI) were purchased from Sigma–Aldrich (Steinheim, Germany). Galantamine, levodopa, DTNB were purchased from Alfa Aesar (Haverhill, MA, USA). DMSO, trifluoroacetic acid, aluminum chloride, potassium phosphate dibasic, potassium phosphate monobasic, potassium acetate were purchased from Carlo Erba (Emmendingen, Germany). Ethanol, sodium carbonate, sodium acetate, sodium phosphate monobasic, sodium phosphate dibasic, potassium chloride were purchased from Merck (Darmstadt, Germany). Cyanidin-3-glucoside was purchased from Extrasynthese (Geney, France). All of the chemicals were of analytical grade and water was obtained with Stakpure Omnia Type I Ultrapure Water Systems (Niederahr, Germany).
Koyu H., Demir S, & Haznedaroglu M.Z. (2023). Investigation of microwave extraction of red cabbage and its neurotherapeutic potential. Journal of Food and Drug Analysis, 31(4), 609-625.
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