Kmc0061

Manufactured by Thermo Fisher Scientific

Sourced in United States

The KMC0061 is a high-performance centrifuge from Thermo Fisher Scientific. It is designed for a range of laboratory applications that require rapid and efficient separation of samples. The centrifuge features a compact and durable construction, allowing it to operate reliably in various laboratory environments.

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Lab products found in correlation

28 protocols using kmc0061

Quantifying Cytokine Responses in STING Knockout Macrophages

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WT and STING^gt/gt BMDMs were seeded at a density of 2 × 10⁵ cells/well in 12-well plates and infected with C. burnetii at 100 GE/cell. The cell-free supernatants (500 µl) were collected at 12 dpi from mock- and C. burnetii-infected WT and STING^gt/gt BMDMs. The supernatants were diluted with assay buffer as per manufacturers protocol and analyzed for the presence of IFNβ (Invitrogen 424001), IL-6 (Invitrogen KMC0061), and TNF-α (Invitrogen BMS607-2HS) using an enzyme-linked immunosorbent assay (ELISA) utilizing paired antibodies according to manufactures protocol.

Chauhan M., Osbron C.A., Koehler H.S, & Goodman A.G. (2024). STING dependent BAX-IRF3 signaling results in apoptosis during late-stage Coxiella burnetii infection. Cell Death & Disease, 15(3), 195.

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Quantifying Il6, Il6r, and Complex

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ELISAs to determine Il6 (Invitrogen, KMC0061, Waltham, MA, USA) levels in cell culture supernatants, Il6 (R&D Systems, M6000B, Minneapolis, MN, USA) or sIl6r (R&D Systems, DY1830) were performed according to the manufacturer’s instructions. The binary Il6:Il6r complex was quantified as previously described.^{25 (link)}

Hoffmann C.J., Kuffner M.T., Koschützke L., Lorenz S., Lips J., Boehm-Sturm P., Mueller S., Koch S.P., An J., Fekonja L.S., Bugaeva P., Rex A., Tünnemann G.L., Endres M, & Harms C. (2023). Zfp580 inactivation as a new therapeutic target to enhance recovery after stroke in mice. Journal of Cerebral Blood Flow & Metabolism, 43(8), 1400-1418.

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Cytokine and Enzyme Assays in Mice

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Based on the manufacturer’s instructions, mouse IL-6 (KMC0061, Invitrogen, Carlsbad, CA, USA), mouse TNF-α (KMC3011, Invitrogen, Carlsbad, CA, USA), mouse IFN-β (439407, BioLegend, Inc. San Diego, CA), human IL-6 (Proteintech, Wuhan, China), and human TNF-α (Proteintech, Wuhan, China) in cell-culture supernatants were measured by commercially available ELISA kits. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations were determined using commercially available kits purchased from Nan Jing Jan Cheng Biochemical Institute (Nanjing, China).

Xie X., Lv H., Liu C., Su X., Yu Z., Song S., Bian H., Tian M., Qin C., Qi J, & Zhu Q. (2021). HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis. BMC Medicine, 19, 247.

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Serum Cytokine and Pannexin-1 Quantification

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Mouse serum was used to determine the levels of IL-6 (Invitrogen, Thermofisher, KMC0061, Carlsbad, CA, USA), TNF-α (Invitrogen, BMS607-3), IL-1β (Invitrogen, BMS6002), and pannexin-1 (PANX1; Abbexa, Cambridge, UK, abx515359). ELISA kits were pre-coated, and the protocols were followed exactly using the mouse serum. The plates were read at 450 nM wavelength and analyzed. Three independent ELISAs were completed with duplicates in each experiment.

Murray J., Martin D.E., Hosking S., Orr-Burks N., Hogan R.J, & Tripp R.A. (2024). Probenecid Inhibits Influenza A(H5N1) and A(H7N9) Viruses In Vitro and in Mice. Viruses, 16(1), 152.

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Metabolic Profile Assessment in Mice

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The fasting blood glucose levels of the mice were examined using a glucometer (One Touch Ultra Easy, Life Scan, Wayne, PA) after 6 hours of fasting, and the fasting serum insulin levels were detected by way of enzyme‐linked immunosorbent assay (ELISA) (Millipore, Billerica, MA) according to the manufacturer's instructions. The triglyceride (TG), total cholesterol (TC), and nonesterified fatty acid (NEFA) levels in serum were examined using commercial kits (290‐63701 for TG, 294‐65801 for TC, 294‐63601 for NEFA; Wako, Tokyo, Japan). The concentrations of serum cytokines and chemokines were measured by way of ELISA (for interleukin [IL]‐1β: ELM‐IL1beta‐001, RayBio (Guangzhou, China); for IL‐6: KMC0061, Invitrogen (Carlsbad, CA, USA); for tumor necrosis factor α [TNF‐α]: ADI‐900‐047, Peprotech (Rocky Hill, NJ, USA); for Mcp‐1: MJE00, R&D Systems (Minneapolis, MN, USA); for IL‐10: M1000B, R&D Systems) according to the manufacturer's instructions.

Lu Y., Jiang Z., Dai H., Miao R., Shu J., Gu H., Liu X., Huang Z., Yang G., Chen A.F., Yuan H., Li Y, & Cai J. (2018). Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway. Hepatology (Baltimore, Md.), 67(4), 1303-1319.

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Cytokine Detection via ELISA

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Serum LPS (Cloud-Clone Crop, IEB526Ge), TNF-α (Elabscience, E-MSEL-M0002), IL-1β (Invitrogen, BMS6002TEN), and IL-6 (Invitrogen, KMC0061) were detected using commercial ELISA kits according to the manufacturer’s protocols.

Luo J.Q., Ren H., Chen M.Y., Zhao Q., Yang N., Liu Q., Gao Y.C., Zhou H.H., Huang W.H, & Zhang W. (2023). Hydrochlorothiazide-induced glucose metabolism disorder is mediated by the gut microbiota via LPS-TLR4-related macrophage polarization. iScience, 26(7), 107130.

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Quantifying Hippocampal Cytokine Levels in Aged Mice

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The concentrations of proinflammatory cytokines IL‐6 (KMC0061, Invitrogen) and TNF‐α (BMS607‐2INST, Invitrogen) in the hippocampus of aged mice were detected using an ELISA assay. The samples were stored in an −80°C refrigerator. The supernatant of the ground samples was used to detect the concentration of IL‐6 and TNF‐α.

Liu L., Liu M., Zhang D., Song Z, & Zhang H. (2023). DHT inhibits REDOX damage and neuroinflammation to reduce PND occurrence in aged mice via mmu_circ_0001442/miR‐125a‐3p/NUFIP2 axis. Brain and Behavior, 13(10), e3180.

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Cytokine Quantification in Serum

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ELISA kits were used to measure TNF-α (pg/mL), IL-1β (pg/mL), and IL-6 (pg/mL) in a serum sample. ( MC3011 for TNF-α; BMS6002 IL-β1; KMC0061 for IL-6) obtained from Invitrogen (USA) 25 .

Rajendran P., Ammar R.B., Al‐Saeedi F.J., Al‐Ramadan S.Y., Ismail M.B., Veeraraghavan V.P., Alamer S.A., Al-Awwad N., Moqbel M.S., & Ahmed E.A. (2022). Geraniol Attenuates Oxidative Stress and Neuro-inflammation Mediated Cognitive Impairment in D‑galactose‑induced Mouse Aging Model.

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Quantitative and Qualitative Assessment of Fas Transcripts

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Quantitative real-time PCR analysis was described previously. 2, 28 Semiquantitative RT-PCR Analysis of mFas and sFas Transcribed cDNA was used as a template for PCR amplification. The paired primers P1 and P2 were used for total Fas amplification and P3 and P5 for mFas/sFas amplification; primer sequences were reported previously. 2 PCR products were analyzed on a 2.5% agarose gel by electrophoresis and ethidium bromide staining. A digital image of the gel was obtained using a SynGene apparatus and the SynGeneGeneSnap software version 4.00.00 (SynGene, San Diego, CA). Individual band intensities were quantitated using the densitometric software Quantity One version 4.6 (Bio-Rad, Hercules, CA). sFas or mFas mRNA level was expressed as a ratio of sFas or mFas PCR product signal to that of glyceraldehyde-3-phosphate dehydrogenase.
ELISA for sFas, TNF-a, and IL-6 sFas in cell culture supernates was detected using a human sFas enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions. Cytokine concentrations in the mice serum were examined by ELISA (ADI-900-047 of Peprotech for TNF-a and KMC0061 of Invitrogen for IL-6). The absorbance of each well was measured at 450 nm using a microplate reader (Bio-Rad). The concentrations of the cytokines were calibrated from a dose-response curve based on reference standards.

Liu W., Guo T.F., Jing Z.T, & Tong Q.Y. (2019). Repression of Death Receptor-Mediated Apoptosis of Hepatocytes by Hepatitis B Virus e Antigen. The American journal of pathology, 189(11).

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Quantification of Immune Mediators by ELISA

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The productions of mouse IL-6 (KMC0061, Novex), TNF-α (KMC3010, Novex), CCL5 (NMR00, R&D Systems), CCL2 (MJE00, R&D Systems) and CXCL10 (BMS6018, Thermo Fisher Scientific) were quantified by ELISA according to the manufacturer’s protocol.

Suzuki T., Okamoto T., Katoh H., Sugiyama Y., Kusakabe S., Tokunaga M., Hirano J., Miyata Y., Fukuhara T., Ikawa M., Satoh T., Yoshio S., Suzuki R., Saijo M., Huang D.C., Kanto T., Akira S, & Matsuura Y. (2018). Infection with flaviviruses requires BCLXL for cell survival. PLoS Pathogens, 14(9), e1007299.

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