Sox1‐GFP+ cells were measured at day 0, 2, 4, and 10 using the Aria II platform and normalized to day 0 (Figure
Ab78078
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab78078 is a laboratory equipment product. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab7260, by Abcam (7 mentions)
Lsm 710, by Zeiss (4 mentions)
Ab150077, by Abcam (3 mentions)
Ab4674, by Abcam (3 mentions)
Fitc labeled goat anti mouse and cy3 labeled goat anti rabbit secondary antibody, by Wuhan Servicebio Technology (3 mentions)
4 6 diamidino 2 phenylindole (dapi), by Wuhan Servicebio Technology (3 mentions)
Ab15580, by Abcam (3 mentions)
Ab5392, by Abcam (3 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (3 mentions)
Ab13970, by Abcam (2 mentions)
Alexa fluor 568, by Thermo Fisher Scientific (2 mentions)
Bz x710, by Keyence (2 mentions)
Ab134017, by Abcam (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Ab18207, by Abcam (2 mentions)
Ab177487, by Abcam (2 mentions)
Ab109186, by Abcam (2 mentions)
Ab68428, by Abcam (2 mentions)
Anti nestin, by Abcam (2 mentions)
72 protocols using ab78078
1
Quantification of Sox1-GFP+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
Sox1‐GFP+ cells were measured at day 0, 2, 4, and 10 using the Aria II platform and normalized to day 0 (FigureS1 C). For Figure S1 B, cells were fixed for 10 minutes with ice‐cold paraformaldehyde and blocked for 30 minutes at 24°C with 0.1% Triton X100‐1% BSA in PBS. Anti‐Oct4 (ab19857, Abcam, Cambridge, Cambridgeshire, United Kingdom, ab19857.html">https://www.abcam.com/oct4-antibody-chip-grade-ab19857.html ), anti‐Nanog (ab80892, Abcam, Cambridge, Cambridgeshire, United Kingdom, ab80892.html">https://www.abcam.com/nanog-antibody-ab80892.html ), anti‐Tubb3 (ab78078, Abcam, Cambridge, Cambridgeshire, United Kingdom, ab78078.html">https://www.abcam.com/beta-iii-tubulin-antibody-2g10-neuronal-marker-ab78078.html ), and anti‐GFP (ab13970, Abcam, Cambridge, Cambridgeshire, United Kingdom, ab13970.html">https://www.abcam.com/gfp-antibody-ab13970.html ) (all at 1:1000) were used, with DAPI as nuclear counterstain (Life Technologies, D1306).
+ Expand
Sox1‐GFP+ cells were measured at day 0, 2, 4, and 10 using the Aria II platform and normalized to day 0 (Figure
2
Immunofluorescence Analysis of Neuronal Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunofluorescence analysis, cell cultures were washed twice with PBS, fixed with 4% paraformaldehyde for 15 min, washed three additional times with PBS, permeabilized with 0.1% Triton X-100 in PBS for 5 min at 37 °C, and blocked for 2 h with 4% albumin. The cells were then incubated overnight with either anti-beta III tubulin (1:400, ab78078, Abcam), anti–HIF–1a (NB100105, Novus), or anti-GFAP (#80788, CST) antibodies at 4 °C, washed with PBS, and incubated with a goat anti-mouse secondary antibody conjugated with either a goat anti-rabbit secondary antibody (Alexa Fluor 488, ab150077 Abcam), goat anti-mouse secondary antibody (Alexa Fluor 488, ab150113, Abcam) or goat anti-rabbit secondary antibody (Alexa Fluor 594, ab150080, Abcam) for 1 h at room temperature. Cell nuclei were stained with 0.2 μg/mL of 4,6-diamino-2-phenylindole (DAPI, D9542, Sigma–Aldrich).
ImageJ software with the Sholl analysis plugin was used to quantify the neurite length, the number of neurites from soma, the number of secondary branches, and the number of neurite intersections according to a previous protocol [25 (link)]. The analyzers were blinded to the neuron groups.
ImageJ software with the Sholl analysis plugin was used to quantify the neurite length, the number of neurites from soma, the number of secondary branches, and the number of neurite intersections according to a previous protocol [25 (link)]. The analyzers were blinded to the neuron groups.
3
Multimodal Neuro-Vascular Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for this study: mouse monoclonal to MHC (Developmental Studies Hybridoma Bank: MF20), mouse monoclonal to β3-Tubulin (abcam: #ab78078), rabbit polyclonal to Col1a1 (abcam: #ab21286), and rabbit polyclonal to CD31 (abcam: #28364). Secondary antibodies used were polyclonal goat anti-mouse and anti-rabbit, coupled with Alexa Fluor® 488 and Alexa Fluor® 568 (Life Technologies: #A11001, #A11004, #A11008, A#11011). Staining was performed according to standard procedures. Due to the species of the antibodies used, the neuro-vasculature analysis was performed using adjacent sections aligned and merged using Adobe Photoshop CS4. The immunofluorescent sections were visualized using Leica DMI 3000B and Keyence BZ-X710 microscopes.
4
Immunohistochemical Assessment of TRPV1 in Human Ocular Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TRPV1 expression in human ocular tissues was evaluated with immunohistochemistry. Anterior segments of human eye globes with no known corneal defects procured during postmortem (Lions Eye Institute, Miami, FL, USA) with consent of donors or donors’ next of kin, in accordance with the Eye Bank Association of America Medical Standards, US/Florida law for human tissue donation, the Declaration of Helsinki and Food and Drug Administration regulations, and Novartis human tissue registration working practice guidelines regarding research using human tissues, were used for immunohistochemical staining. The tissue was fixed in 4% paraformaldehyde for 2 hours and then transferred to 10% sucrose for 1 to 2 hours, 20% sucrose for 2 hours, and 30% sucrose overnight. Then the tissue was flash frozen in optimal cutting temperature compound. Cryosections, 10 µm thick, were fixed with ice-cold acetone for 15 minutes and incubated with TRPV1 antibody (1:100 dilution, ALX-210-417-C100; Enzo Life Sciences, Inc., NY, USA) and β-III tubulin antibody (1:800 dilution, ab78078; Abcam, MA, USA) overnight at 4°C. Secondary donkey anti-rabbit antibody conjugated to Alexa Fluor 594 (1:1000 dilution, A21207; Invitrogen, NY, USA) and goat anti-mouse antibody conjugated to Alexa Fluor 488 (1:1000 dilution, A11001; Invitrogen) were used. Images were captured using the AxioVision Program v4.8.2.
5
Anaerobic Bacteria Impact on Neuronal Cultures
6
Immunofluorescent Staining of Neurospheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neurospheres were permeabilized and blocked with a blocking solution consisting of 5% normal goat serum and 0.5% TritonX-100 in PBS at room temperature for 1 h. Neurospheres were incubated with the primary antibodies diluted in the blocking solution overnight at 4°C and then incubated with the Alexa-conjugated secondary antibodies at room temperature for 1 h. Neurospheres were further stained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min and subsequently mounted onto glass slides with ProLong™ Gold Antifade Mountant (No. P36934, ThermoFisher). Images were acquired on a laser scanning confocal microscope (Leica SP8 TCS) with built-in LAS X software (Leica Biosystem). Images were analyzed with ImageJ software (version 1.45). Primary antibodies and dilutions used in this study were listed in the following: rabbit anti-SOX2 (1:400; #3579; Cell Signaling Technology), mouse anti-Nestin (1:400; #4760; Cell Signaling Technology), rabbit anti-Ki67 (1:100; ab15580; BioLegend), mouse anti-β3-Tubulin (1:200; #4466; Cell Signaling Technology), mouse anti-GFAP (1:1000; ab7260; Abcam), rabbit anti-GFAP (1:5000; ab7260; Abcam), mouse anti-Tuj1 (1:1000; ab78078; Abcam).
7
Immunohistochemical Analysis of Pediatric Brain Tumors
8
Glioblastoma Stem Cell Characterization and LPS Challenge
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glioblastoma cell line U87 was purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). The rat glioma RG2 cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Cells were grown in Dulbecco’s modified essential media (DMEM, Gibco, Gaithersburg, MD, USA) supplemented with 10% FBS (Hyclone, Logan, UT, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin at 37 °C with 5% CO2 in humidified air.
The generation and identification of RG2 and U87 GSCs was performed as we previously described [16 (link)]. The characteristic expression of stem markers CD133 and nestin was assessed by immunofluorescence and western blot using antibodies against CD133 (1:100; PA5-38014, Invitrogen, Carlsbad, CA, USA) and nestin (1:250; ab92391 and ab134017, Abcam, Cambridge, UK). The multi-lineage differentiation capacity of GSCs was examined using anti-glial fibrillary acidic protein (GFAP, ab7260; Abcam) and anti-β III tubulin (ab78078; Abcam).
E. coli 055:B5 LPS (Sigma, St. Louis, MO, USA) was dispersed in phosphate-buffered saline (PBS) at 1 μg/ml. For in vitro assessments, cells were incubated with LPS for 6, 12, or 24 h and washed three times with PBS before further examination. For in vivo experiments, cells were challenged with LPS for 6 h and washed three times with PBS before animal inoculation.
The generation and identification of RG2 and U87 GSCs was performed as we previously described [16 (link)]. The characteristic expression of stem markers CD133 and nestin was assessed by immunofluorescence and western blot using antibodies against CD133 (1:100; PA5-38014, Invitrogen, Carlsbad, CA, USA) and nestin (1:250; ab92391 and ab134017, Abcam, Cambridge, UK). The multi-lineage differentiation capacity of GSCs was examined using anti-glial fibrillary acidic protein (GFAP, ab7260; Abcam) and anti-β III tubulin (ab78078; Abcam).
E. coli 055:B5 LPS (Sigma, St. Louis, MO, USA) was dispersed in phosphate-buffered saline (PBS) at 1 μg/ml. For in vitro assessments, cells were incubated with LPS for 6, 12, or 24 h and washed three times with PBS before further examination. For in vivo experiments, cells were challenged with LPS for 6 h and washed three times with PBS before animal inoculation.
9
Immunolabeling for Myelination Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary antibodies included Anti-beta III Tubulin [2G10] (1:1000, ab78078; Abcam, Cambridge, UK), Recombinant Anti-Cytokeratin 15 (1:200, ab52816; Abcam), Anti-beta III Tubulin (1:300, ab18207; Abcam), Recombinant Anti-SOX10 [EPR4007] (1:100, ab155279; Abcam), Monoclonal Anti-Myelin Basic Protein (MBP, 1:100, MAB386 Sigma-Aldrich), Monoclonal Anti-Sodium Channel, Pan antibody produced in mouse (1:500, S8809; Sigma-Aldrich), and polyclonal Anti-Caspr (1:1000, kindly provided by Elior Peles, PhD, Weizmann Institute of Science, Rehovot, Israel), Recombinant Monoclonal Anti-Mayelin Associated Glycoprotein [EPR24276] (MAG, 1:500, ab277524, Abcam).
10
Immunohistochemical Analysis of Neural Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen sections were rinsed in phosphate-buffered saline (PBS) and then blocked in PBS containing 0.3% Triton X-100 and 1% donkey serum for 30 min. The sections were incubated with the primary antibodies at 4°C overnight. The following primary antibodies were used: anti-NeuN (1:100, ab177487, Abcam), anti-GFAP (1:400, ab4674, Abcam), anti-C3 (1:300, ab200999, Abcam), anti-S100a10 (1:200, PA5-95505, Invitrogen), and anti-beta III tubulin (1:400, ab78078, Abcam). After washing with PBS, the sections were incubated with appropriate secondary antibodies at 37°C for 2 h and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) the next day. Fluorescence images were acquired with a fluorescence microscope (BX51, Olympus). Three non-consecutive sections (imaged using a 20× objective lens) from each subject and five separate visual fields from each section were randomly selected and analyzed. All images were analyzed using ImageJ (NIH), and all analyses were performed in a blinded manner.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!