Tellurium powder (Te), sodium hydroxide (NaOH), sodium borohydride (NaBH
4),
cadmium chloride (CdCl
2),
thioglycolic acid (TGA),
glutathione (GSH), zinc chloride, sodium chloride (NaCl), magnesium chloride (MgCl
2), and tris hydrochloride (Tris-HCl) were purchased from Merck, Sigma Aldrich or Carloerba Reagents companies. All DNA probes were prepared from Metabion, Germany, with the following sequences:
Capture DNA (thiolated DNA): 5′-Thiol -C6-GTACTGTAGGC-3′ (11-mer)
Quencher DNA (BHQ
2- DNA): 5′-CGGCACTTGTG-BHQ
2-3′ (11-mer)
Complementary DNA (target DNA): 5′-CACAAGTGCCGGCCTACAGTAC-3′ (22-mer: This arrangement is planned based on a specific sequence of the Covid-19 virus genome) [19] , [20] (
link)
Non-complementary DNA (non-target DNA): 5′-GTGACATGACATCCGTTCGTGA −3′ (22-mer)
The RNase-free water and RNA of Covide-19 viruses were provided by National Institute for Genetic Engineering and Biotechnology (NIGEB), Iran. Double distilled water was used throughout the analysis.
Bardajee G.R., Zamani M., Mahmoodian H., Elmizadeh H., Yari H., Jouyandeh L., Shirkavand R, & Sharifi M. (2021). Capability of novel fluorescence DNA-conjugated CdTe/ZnS quantum dots nanoprobe for COVID-19 sensing. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 269, 120702.