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2 propanol

Manufactured by Carl Roth
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Sourced in Germany, United States

2-propanol is a clear, colorless liquid chemical compound. It is commonly used as a solvent and reagent in various laboratory applications.

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Lab products found in correlation

Acetone, by Carl Roth (5 mentions) Ammonium formate, by Merck Group (4 mentions) Chloroform, by Carl Roth (4 mentions) Methanol, by Merck Group (4 mentions) Formic acid, by Carl Roth (4 mentions) Toluene, by Merck Group (4 mentions) Ethanol, by Carl Roth (4 mentions) Hplc grade methanol, by Carl Roth (3 mentions) Acetic acid, by Carl Roth (3 mentions) Methanol, by Carl Roth (3 mentions) Roti histofix 4, by Carl Roth (2 mentions) Chloroform, by Merck Group (2 mentions) Diphenyl 2 4 6 trimethylbenzoyl phosphine oxide tpo, by Merck Group (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (2 mentions) Nuclease free water, by B. Braun (2 mentions) Cholesteryl ester, by Merck Group (2 mentions) Triglyceride tg, by Larodan (2 mentions) Diglyceride dg, by Larodan (2 mentions) Lysophosphatidylethanolamine lpe, by Avanti Polar Lipids (2 mentions) Calf intestine alkaline phosphatase, by Merck Group (2 mentions)

46 protocols using 2 propanol

1

Quantitative Lipid Analysis of Hepatocellular Carcinoma

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Lipids are another means of nutrient storage, and they deliver structural components for cell proliferation. Hepatocellular carcinoma cells exhibit not only a reprogramming of glucose but also of lipid metabolism [29 (link)]. To visualize and quantify intracellular lipid contents in the cells, Oil Red O staining was performed. This diazo dye (Sigma–Aldrich, St. Louis, MO, USA) is able to bind to neutral lipids and stains them red. The cells were washed and fixed with Roti®-Histofix 4% (Carl Roth, Karlsruhe, Germany) for 30 min. Then, the cells were washed with 60% 2-propanol (Carl Roth, Karlsruhe, Germany), dried and incubated with Oil Red O working solution (0.35% Oil Red O in 2-propanol, diluted in dH2O in a 3:2 ratio) for 20 min. The staining solution was discarded and the nonfixed dye was removed by washing three times with dH2O. Lipid staining was evaluated using a light microscope (Eclipse TS100, Nikon, Tokyo, Japan) and the cells were then air-dried. The Oil Red O stain was extracted with 100% 2-propanol and the absorbance was measured at 492 nm with a microplate reader (Synergy H1, BioTek, Winooski, VT, USA).
Schicht G., Seidemann L., Haensel R., Seehofer D, & Damm G. (2022). Critical Investigation of the Usability of Hepatoma Cell Lines HepG2 and Huh7 as Models for the Metabolic Representation of Resectable Hepatocellular Carcinoma. Cancers, 14(17), 4227.
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2

Fabrication of Silica-Polymer Nanocomposites

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Amorphous silica nanopowder of type Aerosil OX50 was kindly provided by Evonik, Germany. Hydroxyethylmethacrylate (HEMA) was purchased from Alfa Aesar, Germany. Tetraethylenglycoldiacrylate (TEGDA), polyethylenglycoldiacrylate 550 (PEGDA-550), phenylbis(2,4,6-trimethylbenzoyl)phosphine oxide, propylene glycol methyl ether acetate (PGMEA), and 2.2-dimethoxy-2-phenylacetophenone (DMPAP) were purchased from Sigma–Aldrich. Negative-tone photoresists IP-S was purchased from Nanoscribe, Germany. 2-propanol was purchased from Carl Roth, Germany.
Kotz F., Risch P., Arnold K., Sevim S., Puigmartí-Luis J., Quick A., Thiel M., Hrynevich A., Dalton P.D., Helmer D, & Rapp B.E. (2019). Fabrication of arbitrary three-dimensional suspended hollow microstructures in transparent fused silica glass. Nature Communications, 10, 1439.
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3

Synthesis of Fluorinated Polymer Coatings

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Fluorolink MD700 (a perfluoropolyether (PFPE)-methacrylate) was purchased from Acota (United Kingdom), 1H,1,2H,2H-perfluorooctanol (13FOOl) was purchased from Apollo Scientific (United Kingdom). Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) and Sudan 3 were purchased from Sigma-Aldrich (Germany). Cyclohexanol, cyclohexane and chloroform were purchased from Merck (Germany). Acetone and 2-propanol were purchased from Carl Roth (Germany). Tinuvin 384-2 was kindly provided by BASF (Germany).
Mayoussi F., Doeven E.H., Kick A., Goralczyk A., Thomann Y., Risch P., Guijt R.M., Kotz F., Helmer D, & Rapp B.E. (2021). Facile fabrication of micro-/nanostructured, superhydrophobic membranes with adjustable porosity by 3D printing. Journal of Materials Chemistry. a, 9(37), 21379-21386.
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4

DNA Origami Structures Characterization

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Glass slides were sonicated for 5 min with HellmanexIII (Sigma-Aldrich), washed 5 x with double distilled water and sonicated for 5 min each with Acetone (Roth) and 2-Propanol (Roth). Clean glass slides were blown dry with nitrogen and glow discharged under oxygen in a plasma cleaner (Binder) for 5 min. In order to locate the sample in the center of the glass slide a circle was drawn with a grease pencil on the glass slide. Open and closed state DNA origami structures with AuNPs attached (50 pM) were deposited inside the grease circle for one minute, washed two times with 1 ml doubly distilled water and blown dry with nitrogen. Samples for Darkfield microscopy were imaged with a home-built dark-field setup in transmission mode using a 100 x air objective (Olympus) and an oil condenser (Olympus NA 1.4) with a 100 W halogen bulb as illumination source. Images were taken with 100 ms exposure time and 2 x binning on a color CMOS Camera (Thorlabs Kiralux CS895CU). The dark-field scattering spectra were collected with the same home-built dark-field coupled to an Acton SP2300 spectrometer (Princeton Instruments). To analyze the RGB intensity values of individual nanostructures, the images were first thresholded and individual spots then automatically detected.
Gür F.N., Kempter S., Schueder F., Sikeler C., Urban M.J., Jungmann R., Nickels P.C, & Liedl T. (2021). Double- to Single-Strand Transition Induces Forces and Motion in DNA Origami nanostructures. Advanced materials (Deerfield Beach, Fla.), 33(37), e2101986.
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5

Transition Metal Chloride Synthesis

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N-Butylpyridinium chloride (≥98%, Merck, CAS), CuCl2 (99.995%, ABCR, CAS), CoCl2 (99.95%, ABCR, CAS), MnCl2 (99.95%, ABCR, CAS), NiCl2 (99.95%, ABCR, CAS), ZnCl2 (99.95%, ABCR, CAS), 2-propanol (99.5%, Carl Roth, CAS) and hydrochloric acid (37%, VWR Chemicals, CAS) were used without further purification.
Behrens K., Balischewski C., Sperlich E., Menski A., Balderas-Valadez R.F., Pacholski C., Günter C., Lubahn S., Kelling A, & Taubert A. (2022). Mixed chloridometallate(ii) ionic liquids with tunable color and optical response for potential ammonia sensors. RSC Advances, 12(54), 35072-35082.
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6

Glycerolipid Standards Characterization

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Methanol and ethanol absolute (EMSURE) were obtained from Merck (Darmstadt, Germany), and chloroform and 2-propanol from Roth (Karlsruhe, Germany). All solvents were of HPLC grade. Ammonium formate was ordered from Sigma-Aldrich (Taufkirchen, Germany) and isooctane (2,2,4-trimethylpentane) > 99% from Honeywell (Seelze, Germany). All chemicals and standards were of high purity grade for analysis (> 95%). Glycerolipid standards were purchased from Larodan (Solna, Sweden): diarachidin (DG 20:0/20:0), dinonadecanoin (DG 19:0/19:0), dilinolenin (DG 18:3/18:3), dilinolein (DG 18:2/18:2), 1,2-distearin (DG 18:0/18:0), triarachidin (TG 20:0/20:0/20:0), trinonadecanoin (TG 19:0/19:0/19:0), trilinolein (TG 18:2/18:2/18:2), triolein (TG 18:1/18:1/18:1), 1,2-olein-3-stearin (TG 18:1/18:1/18:0), 1,2-stearin-3-olein (TG 18:0/18:0/18:1), triheptadecanoin (TG 17:0/17:0/17:0), and tripalmitin (TG 16:0/16:0/16:0). Purified water was produced by Millipore Milli-Q UF-Plus water purification system (Molsheim, France).
Ertl V.M., Höring M., Schött H.F., Blücher C., Kjølbæk L., Astrup A., Burkhardt R, & Liebisch G. (2020). Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry. Analytical and Bioanalytical Chemistry, 412(10), 2315-2326.
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7

Microfluidic Chip Substrate Preparation

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Unmodified injection molded cyclo-olefin polymers (COP) substrates (microfluidic ChipShop GmbH, Jena, Germany) comprising a 36 mm long channel with a width of 3000 μm and a depth of 200 μm and a subsequently connected 75 μl collection chamber was used. As the microarray is immobilized in the channel and the channel volume is 21.6 μl, the sample volume is set to 25 μl ensuring complete filling of the channel. The substrates were initially cleaned with 50% (v/v) 2-Propanol (99.8% p.a., Carl Roth GmbH + Co. KG, Germany) and deionized (DI) water in a ultrasonic bath (Sonorex Super RK 100 H, Bandelinelectronic GmbH & Co. KG, German) for 5 minutes to remove impurities and residues, and was subsequently dried with a nitrogen jet and stored at 4°C in a dark place until usage.
Stumpf A., Brandstetter T., Hübner J, & Rühe J. (2019). Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum. PLoS ONE, 14(12), e0225525.
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8

Lipid Extraction and Quantification

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Chloroform and 2-propanol were purchased from Roth (Karlsruhe, Germany) and methanol from Merck (Darmstadt, Germany). All solvents were HPLC grade. Nuclease-free water was obtained from B. Braun (Melsungen, Germany). Ammonium formate, sodium dodecyl sulfate (SDS), and cholesteryl ester (CE) standards were purchased from Sigma-Aldrich (Taufkirchen, Germany). Moreover, [25,26,26,26,27,27,27-D7]-cholesterol was acquired from Cambridge Isotope Laboratories (Andover, MA, USA) with isotope purity higher than 98%. Triglyceride (TG) and diglyceride (DG) standards were purchased from Larodan (Solna, Sweden). Phosphatidylcholine (PC), ceramide (Cer), sphingomyelin (SM), lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE) standards were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The composition of the added internal standard mixture is depicted in Table 1.
Höring M., Krautbauer S., Hiltl L., Babl V., Sigruener A., Burkhardt R, & Liebisch G. (2021). Accurate Lipid Quantification of Tissue Homogenates Requires Suitable Sample Concentration, Solvent Composition, and Homogenization Procedure—A Case Study in Murine Liver. Metabolites, 11(6), 365.
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9

Enzymatic Nucleic Acid Manipulation Protocol

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Calf
intestine alkaline phosphatase, micrococcal
nuclease (from Staphylococcus aureus), bovine spleen
phosphodiesterase, and ribonuclease A from bovine pancreas (RNase)
were purchased from Sigma-Aldrich (Steinheim, Germany). Proteinase
K, HPLC-grade methanol, 2-propanol, 1-butanol, formic acid, and acetic
acid were from Carl Roth GmbH (Karlsruhe, Germany). Herring sperm
DNA and all other reagents and solvents (analytical grade) were from
Sigma-Aldrich.
Monien B.H., Schumacher F., Herrmann K., Glatt H., Turesky R.J, & Chesné C. (2014). Simultaneous Detection of Multiple DNA Adducts in Human Lung Samples by Isotope-Dilution UPLC-MS/MS. Analytical Chemistry, 87(1), 641-648.
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10

Synthesis of Photocurable Biomaterials

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Poly(ethylene glycol) (PEG) (Mn~10,000), PEG (Mn~400), Pentaerythritol ethoxylate (15/4 EO/OH) (Mn~797), methacrylic anhydride, acrylic acid, 4-dimethylaminopyridine (DMAP), PEGDA (Mn~575), isobornyl acrylate, diurethane dimethacrylate (DUDMA), diphenyl(2,4,6-trimethylbenzoyl) phosphine oxide (TPO), dibutyltin dilaurate, and pentaerythritol tetraacrylate (PETA) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Tetrahydrofuran, diethyl ether, and 2-propanol were purchased from Carl Roth. Tinuvin-326 was kindly provided by BASF (Ludwigshafen, Germany).
Song Q., Chen Y., Hou P., Zhu P., Helmer D., Kotz-Helmer F, & Rapp B.E. (2023). Fabrication of Multi-Material Pneumatic Actuators and Microactuators Using Stereolithography. Micromachines, 14(2), 244.
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