Goat anti mouse igg hrp sc 2005

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Germany, China

Goat anti-mouse IgG-HRP (sc-2005) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize primary antibodies raised in mouse.

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Lab products found in correlation

Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (37 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (7 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (5 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (5 mentions) Protran nitrocellulose membrane, by GE Healthcare (4 mentions) Np40 cell lysis buffer, by Thermo Fisher Scientific (4 mentions) Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (4 mentions) Pol β specific antibody ab26343, by Abcam (3 mentions) Alexa fluor 488 donkey anti rabbit igg antibody a21206, by Thermo Fisher Scientific (3 mentions) Ripa buffer, by Beyotime (3 mentions) Donkey anti goat igg hrp sc 2020, by Santa Cruz Biotechnology (3 mentions) Hybond ecl nitrocellulose membrane, by GE Healthcare (3 mentions) Goat anti rabbit igg hrp sc 2054, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 594 donkey anti mouse igg, by Thermo Fisher Scientific (2 mentions) Donkey anti goat igg hrp, by Santa Cruz Biotechnology (2 mentions) Protease and phosphatase inhibitors cocktail, by Roche (2 mentions) Ripa lysis buffer 10, by Merck Group (2 mentions) Goat polyclonal anti rabbit igg hrp, by Abcam (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle medium dmem, by Merck Group (2 mentions)

Close spelling variants of this product

Goat anti-mouse IgG-horseradish peroxidase (HRP) antibody (sc-2005 (2 citations) Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-2005), (4 citations) Goat anti-mouse IgG HRP-conjugated (sc-2005, (6 citations) Anti-mouse IgG-HRP (sc–2005 (4 citations) Goat anti-mouse IgG (sc-2005) (7 citations) Goat anti-mouse (sc-2005) (22 citations) Anti-mouse IgG (sc-2005) (6 citations) Anti-mouse (sc-2005, (13 citations) HRP-anti-goat IgG (2 citations) Goat anti-mouse HRP (51 citations)

87 protocols using goat anti mouse igg hrp sc 2005

Mitochondrial Dynamics Antibodies Protocols

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The following primary antibodies were used: DRP1 (mouse (WB: 1:500)) and pDRP1 (S616) (3455 S, rabbit (WB 1:1000, IF: 1:500)) from Cell Signalling Technology (Danvers, Massachusetts, USA); OPA1 (612607, mouse (WB 1:1000, IF: 1:750)) from BD Biosciences (Mississauga, Ontario, Canada); MFN1 (rabbit (WB: 1:1000) from ThermoFisher Scientific (Mississauga, Ontario, Canada); acid ceramidase (ASAH1) (OAPB00726, rabbit (WB 1:1000) from Aviva Systems Biology (Cedarlane, Burlington, Ontario, Canada); and ACTB (β-actin; I-19, sc-1616, goat (WB 1:2000)) from Santa Cruz Biotechnology (Mississauga, Ontario, Canada). Secondary antibodies included goat anti-rabbit IgG-HRP (sc-2054 (WB: 1:2000)) and goat anti-mouse IgG-HRP (sc- 2005 (WB: 1:2000)) from Santa Cruz Biotechnology. For IF experiments, Alexa Fluor 488 donkey anti-rabbit IgG (A21206), Alexa Fluor 594 donkey anti-rabbit IgG (A21207) and Alexa Fluor 594 donkey anti-mouse IgG (A21203) by ThermoFisher Scientific were used.

Abbade J., Klemetti M.M., Farrell A., Ermini L., Gillmore T., Sallais J., Tagliaferro A., Post M, & Caniggia I. (2020). Increased placental mitochondrial fusion in gestational diabetes mellitus: an adaptive mechanism to optimize feto-placental metabolic homeostasis?. BMJ Open Diabetes Research & Care, 8(1), e000923.

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Western Blot Analysis of Autophagy and Inflammatory Markers

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Western blot analysis was performed as described previously[9 (link)]. Briefly, cultured cells were homogenized in a lysis buffer containing a protease inhibitor cocktail. After SDS-PAGE, the proteins on the gel were transferred to a polyvinylidene membrane. Then, the membrane was stained with the desired antibodies. The membrane was visualized with ImmobilonWestern (Millipore, Hayward, CA), and the image was captured with ChemDoc XRS (Bio-Rad, Hercules, CA). Primary antibodies used for Western blotting: microtubule-associated protein 1 light chain 3 (LC3) rabbit polyclonal antibody (PM036; MBL, Nagoya, Japan), p62 mouse monoclonal antibody (ab56416; Abcam, Cambridge, UK), PARP1 rabbit polyclonal antibody (9542S; Cell Signaling Technology, Danvers, MA), phospho-NF-kB p65 rabbit monoclonal antibody (Ser536) (3033S; Cell Signaling Technology), NF-kB p65 rabbit polyclonal antibody (sc-372; Santa Cruz Biotech, Dallas, TX), phosphor-extracellular signal–regulated kinase (ERK) rabbit polyclonal antibody (9101S; Cell Signaling Technology), ERK rabbit monoclonal antibody (4695S; Cell Signaling Technology), tubulin rabbit monoclonal antibody (ab176560; Abcam). Goat anti-rabbit IgG-HRP (sc-2030; Santa Cruz) or goat anti-mouse IgG-HRP (sc-2005; Santa Cruz) were used as secondary antibodies.

Kubota M., Kakimoto K., Nakagawa T., Koubayashi E., Nakazawa K., Tawa H., Hirata Y., Okada T., Kawakami K., Asai A., Hosomi S., Takeuchi T., Fukunishi S., Inoue T., Asahi M, & Higuchi K. (2019). Autophagy deficiency exacerbates colitis through excessive oxidative stress and MAPK signaling pathway activation. PLoS ONE, 14(11), e0225066.

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Western Blot Analysis of Protein Levels

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Whole cell lysates were prepared using EBC₂₅₀ lysis buffer (50 mM Tris-HCl pH 8.0, 250 mM NaCl, 0.5% Nonidet P-40, 1 mM PMSF, 2 µg/mL aprotinin, 40 mM NaF, and 0.5 mM NaVO₄). Equal amounts of total protein were separated by SDS-PAGE and then transferred onto PVDF membranes and hybridized to a specific primary antibody and HRP-conjugated secondary antibody for subsequent detection by ECL (Pierce). Primary antibodies and dilutions used: Rb 1:500 (G3-245, BD Bioscience); myogenin 1:400 (F5D, Santa Cruz); actin 1:1000 (C-11, Santa Cruz); murine MDM2 (mixture of 2A10, 4B2, and 4B11; 1.0 mL each in 20 mL), and MyHC 1:400 (F-20, DSHB). Secondary antibodies were purchased from Santa Cruz Biotechnology and used at 1:3000 dilutions, including goat anti-mouse IgG-HRP (sc-2005), goat anti-rabbit IgG-HRP (sc-2004), and donkey anti-goat IgG-HRP (sc-2020).

Walsh E.M., Niu M., Bergholz J, & Xiao Z.X. (2015). Nutlin-3 down-regulates Retinoblastoma protein expression and inhibits muscle cell differentiation. Biochemical and biophysical research communications, 461(2), 293-299.

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Regulation of mTOR Signaling Pathway

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The following were purchased from Sigma-Aldrich (Dorset, UK): Dulbecco’s Modified Eagle Medium (DMEM), Penicillin/Streptomycin, MEM non-essential amino acids 100×, 0.25% Trypsin-EDTA, L-glutamine, putrescine, spermidine, spermine. Fetal bovine serum (FBS) was from Gibco by Life Technologies (Sao Paolo, Brazil), mTOR siRNA and negative control were from Fisher Chemical (Loughborough, UK), protease and phosphatase inhibitors cocktail from Roche (Indianapolis, IN, USA), BCA protein assay kit from BioVision (Milpitas, CA, USA). RIPA lysis buffer 10× from Millipore (Burlinton, MA, USA). The following antibodies were purchased from Abcam Biotechnology (Cambridge, UK) and used at the indicated dilutions: rabbit polyclonal anti-actin antibody (ab119716, 1:7, 500 dilution), goat polyclonal anti-rabbit IgG-HRP (ab205718, 1:10,000 dilution). Additionally, mouse monoclonal anti-ODC antibody (sc-398116, 1:100 dilution), mouse monoclonal anti-phospho-4EBP1 (sc-293124, 1:200 dilution), mouse monoclonal anti-phospo-p70S6K (sc-8416, 1:300 dilution), mouse monoclonal anti- eIF4E (sc-271480, 1:300 dilution) and goat anti-mouse IgG-HRP (sc-2005, 1:2,500 dilution) antibodies were from Santa-Cruz Biotechnology (Heidelberg, Germany). Rapamycin and the class 1 dual inhibitors of PI3K and mTORC1, NVP-BEZ235, were from Cayman Chemicals (Ann Arbor, MI, USA).

Akinyele O, & Wallace H.M. (2022). Understanding the Polyamine and mTOR Pathway Interaction in Breast Cancer Cell Growth. Medical Sciences, 10(3), 51.

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Fanconi Anemia Pathway Protein Analysis

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Primary antibodies used were FANCA (A301-980A) (1:2000), FANCD1 (A300-005A) (1:1000), FANCD2 (A302-174A) (1:2000), FANCE (A302-125A) (1:1000), FANCI (A300-212A) (1:700), FANCM (A302-637A) (1:1000), FANCO (A302-645A) (1:1000), FANCP (A302-270A) (1:1000), FANCQ (A301-315A) (1:1000), H2AX (A300-082A) (1:5000) from Bethyl Laboratories; FANCF (PA5-18202) (1:1000), FANCG (PA5-27117) (1:1000), FANCL (PA5-19332) (1:1000), PIK3C2A (MA526505) (1:1000), and TFRC (136800) (1:1000) were from ThermoFisher; β-actin (sc-47778) (1:2000) was from Santa Cruz Biotechnology; FANCS (OP92-100UG) was from Calbiochem; γ-H2AX (NB100-78356) (1:5000) was from Novus Biologicals. Secondary antibodies used were goat anti-rabbit IgG-HRP (sc-2357) (1:5000), goat anti-mouse IgG-HRP (sc-2005) (1:5000), and goat anti-rat IgG-HRP (sc-2303) (1:5000) were from Santa Cruz Biotechnology.

Lagundžin D., Hu W.F., Law H.C., Krieger K.L., Qiao F., Clement E.J., Drincic A.T., Nedić O., Naldrett M.J., Alvarez S, & Woods N.T. (2019). Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome. PLoS ONE, 14(8), e0220568.

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Protein Expression Analysis in T-Cells

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Whole cell lysates were prepared from T-lymphoblasts, total protein was resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti-OAS1 (sc-374656, 1:1,000), anti-Actin (sc-8432, 1:1,000) and goat anti-mouse IgG-HRP (sc-2005, 1:10,000, Santa Cruz, USA) as previously described (54 (link)).

Magg T., Okano T., Koenig L.M., Boehmer D.F., Schwartz S.L., Inoue K., Heimall J., Licciardi F., Ley-Zaporozhan J., Ferdman R.M., Caballero-Oteyza A., Park E.N., Calderon B.M., Dey D., Kanegane H., Cho K., Montin D., Reiter K., Griese M., Albert M.H., Rohlfs M., Gray P., Walz C., Conn G.L., Sullivan K.E., Klein C., Morio T, & Hauck F. (2021). Heterozygous OAS1 Gain-of-Function Variants Cause an Autoinflammatory Immunodeficiency. Science immunology, 6(60), eabf9564.

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Antibody Validation for Western Blotting

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β-Actin peroxidase antibody (A3854) were purchased from Sigma (St Louis, MO, USA); p53 (sc-126), HIF1 alpha (sc-10790), Pol II (sc-899), LSm8 (sc-404816) antibodies, and goat anti-mouse IgG-HRP (sc-2005) were from Santa Cruz (Dallas, TX, USA); hDBR1 (H00051163-B01P), SF3A1 (NB100-79843), PRPF8 (NBP2-22274), PRP19 (H00027339-A01) and DHX35 (NBP1-57348) antibodies were from Novus Biologicals (Littleton, CO, USA). Rabbit IgG-HRP secondary antibody (A120-101 P) was from Bethyl Laboratories (Montgomery, TX, USA). Alexa Fluor 568 rabbit IgG (H+L) secondary antibody (A-11011) and Alexa Fluor 488 mouse IgG (H+L) secondary antibody (A-11001) were from Thermo Fisher Scientific (Waltham, MA, USA).

Han B., Park H.K., Ching T., Panneerselvam J., Wang H., Shen Y., Zhang J., Li L., Che R., Garmire L, & Fei P. (2017). Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. Oncogene, 36(38), 5382-5391.

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Western Blot Analysis of EZH2 Expression

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Protein extraction and western-blotting were performed as previously described^{13 (link)}. Briefly, total proteins were extracted from cell layer using radio-immunoprecipitation assay (RIPA) lysis buffer supplemented with phosphatase and protease inhibitors. Then, 30 μg proteins were subjected to fractionation by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride membranes (Bio-Rad), and incubated with antibodies. The following antibodies were used: anti-EZH2 antibody (D2C9) from Cell signaling, anti-β-actin antibody (sc-47778), goat anti-mouse IgG-HRP (sc-2005) and goat anti-rabbit IgG-HRP (sc-516087) from Santa Cruz Biotechnology.

Allas L., Brochard S., Rochoux Q., Ribet J., Dujarrier C., Veyssiere A., Aury-Landas J., Grard O., Leclercq S., Vivien D., Ea H.K., Maubert E., Cohen-Solal M., Boumediene K., Agin V, & Baugé C. (2020). EZH2 inhibition reduces cartilage loss and functional impairment related to osteoarthritis. Scientific Reports, 10, 19577.

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Protein Extraction and Western Blot Analysis

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Asynchronous cells growing at 30°C were incubated with 0.01% MMS. Cells were collected at different time points up to 4 h. Total protein was extracted using trichloroacetic acid (46 (link),47 (link)), separated on polyacrylamide gels, and transferred to Hybond ECL nitrocellulose membranes (GE Healthcare, Little Chalfont, UK). Membranes were incubated with primary antibodies for 1 h at room temperature, washed, and then incubated with secondary antibodies for 1 h. Chemiluminescence was performed with Amersham ECL Prime (GE Healthcare) and chemiluminescence detection and band intensity quantification were achieved using an ImageQuant LAS 4000 imager (GE Healthcare). The primary antibodies used were: α-HA (12CA5, Roche Applied Science; Basel, Switzerland), α-GFP (1181446001, Roche Applied Science), α-H3K36me2 (CS-127-100, Diagenode), α-H3K36me3 (ab9050, Abcam) and α-Cdc2 (sc-53, Santa Cruz Biotechnology; Dallas, TX, USA). The secondary antibodies, goat-anti-mouse IgG-HRP (sc-2005) and goat-anti-rabbit IgG-HRP (sc-2004), were from Santa Cruz Biotechnology.

Lim K.K., Nguyen T.T., Li A.Y., Yeo Y.P, & Chen E.S. (2018). Histone H3 lysine 36 methyltransferase mobilizes NER factors to regulate tolerance against alkylation damage in fission yeast. Nucleic Acids Research, 46(10), 5061-5074.

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Elucidating Molecular Mechanisms of FXR Signaling

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CDCA, GW4064, and BrdU were purchased from Sigma Chemicals (CA, USA). Luciferase assay system was purchased from Promega (WI, USA). EMSA assay kit and ChIP assay kit were purchased from Beyotime (Shanghai, China). Antibodies against FXR (sc-13063X), Numb (sc-136554), rabbit immunoglobulin G-horseradish peroxidase (IgG-HRP) (sc-2004), and goat anti-mouse IgG-HRP (sc-2005) were purchased from Santa Cruz (CA, USA). Antibody against Notch1 (#4380) and NICD1 (#4147) was purchased from Cell Signaling Technology (MA, USA). CD133 (ab19898) antibody was purchased from Abcam (MA, USA). Sox9 (AB5535) and BrdU (MAB3424) antibodies were purchased from Millipore (MA, USA). CD133-phycoerythrin (372803) antibody was purchased from BD Biosciences (CA, USA). Antibody against alpha-Tubulin (66031-1-Ig), LaminB (66095-1-Ig) was purchased from Proteintech (Wuhan, China). GAPDH antibody (BM-1623) was purchased from the Boster Biological Technology (Wuhan, China).

Chen M., Lu C., Lu H., Zhang J., Qin D., Liu S., Li X, & Zhang L. (2021). Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model. Stem Cell Research & Therapy, 12, 232.

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