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Swiss webster

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The Swiss Webster mouse is a widely used outbred mouse model. It is a general-purpose model suitable for a variety of research applications.

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Lab products found in correlation

C57bl 6n, by Taconic Biosciences (2 mentions) C57bl 6j, by Charles River Laboratories (2 mentions) Dba 2j mice, by Charles River Laboratories (2 mentions) 129s6 svevtac mice, by Taconic Biosciences (2 mentions) C57bl 6 mice, by Jackson ImmunoResearch (2 mentions) Balb c mice, by Taconic Biosciences (2 mentions) Male athymic ncr nude, by Taconic Biosciences (2 mentions) Nod scid gamma mouse, by Jackson ImmunoResearch (2 mentions) Vi cell xr, by Beckman Coulter (2 mentions) Nod scid il2rgammanull mice, by Jackson ImmunoResearch (2 mentions) C57bl 6 and cd1, by Taconic Biosciences (2 mentions) Cuy21 electroporator, by Nepa Gene (2 mentions) Ecm 830 electroporator, by Harvard Apparatus (2 mentions) Black swiss, by Taconic Biosciences (2 mentions) E18 sprague dawley rat embryos, by Charles River Laboratories (2 mentions) Ubc gfp c57bl 6 tg ubc gfp 30scha j, by Jackson ImmunoResearch (2 mentions) R26r yfp b6 cg gt rosa 26sortm3 cag eyfp hze j, by Jackson ImmunoResearch (2 mentions) R2g2 mice b6 129 rag2tm1fwail2rgtm1rsky dwihsd, by Inotiv (2 mentions) Nod scid mice nod cg prkdcscid j, by Jackson ImmunoResearch (2 mentions) Jax 001303, by Jackson ImmunoResearch (2 mentions)

Spelling variants of this product

Male Swiss Webster mice (10 citations) Germ-free Swiss Webster mice (8 citations) Swiss Webster female mice (7 citations) Swiss Webster pregnant female mice (4 citations) Gnotobiotic Swiss Webster germ-free mice (2 citations) Germ-Free (GF) Swiss Webster (2 citations) GF Swiss Webster mice (2 citations) Gnotobiotic Swiss Webster mice (Tac:SW) (2 citations) GF wildtype Swiss Webster mice (2 citations) Swiss Webster females (2 citations)

24 protocols using swiss webster

1

Evaluating Mouse Strain Sensitivity for CIPN and Pain Assays

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Male C57Bl/6 (CIPN, reinforcement studies) and Swiss Webster (acetic acid-induced stretching, hot plate) mice were purchased from Taconic Farms (Cranbury NJ, USA) weighing 18-20 g for C57's and 20-25 g for Swiss Webster's upon arrival. C57Bl/6 mice have historically been used in our laboratory for all CIPN experiments based on an initial report by Smith et al. (2004) determining relative sensitivity to paclitaxel-induced nociceptive behaviours across mouse strains and follow-up work in house. At the end of all experiments mice were killed by CO 2 asphyxiation followed by cervical dislocation. Mice were not reused. Swiss Webster mice were used for hot plate and acetic acid tests because of their relative sensitivities to morphine antinociception in the hot plate and acetic acid as compared with C57's. Mice were group housed in plastic cages and allowed to acclimate to the temperaturecontrolled and humidity-controlled animal facility for 5 days before the experiments began. Mice had free access to food and water except where noted in the operant behaviour assay procedures (see below).
Foss J.D., Farkas D.J., Huynh L.M., Kinney W.A., Brenneman D.E, & Ward S.J. (2021). Behavioural and pharmacological effects of cannabidiol (CBD) and the cannabidiol analogue KLS-13019 in mouse models of pain and reinforcement. British journal of pharmacology, 178(15).
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2

Ocular P. aeruginosa Infection in Mice

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Mice were housed and bred at the MCP Animal Care Facility. Swiss Webster (SW) and C57BL/6N mice were purchased from Taconic Farms. Seven to nine-week old mice were used throughout the experiments. We have not observed significant differences in the bacterial clearance of male and female mice to ocular P. aeruginosa–induced infection.
Kugadas A., Geddes‐McAlister J., Guy E., DiGiandomenico A., Sykes D.B., Mansour M.K., Mirchev R, & Gadjeva M. (2019). Frontline Science: Employing enzymatic treatment options for management of ocular biofilm‐based infections. Journal of Leukocyte Biology, 105(6), 1099-1110.
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3

Ocular P. aeruginosa Infection in Mice

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Mice were housed and bred at the MCP Animal Care Facility. Swiss Webster (SW) and C57BL/6N mice were purchased from Taconic Farms (Rensselaer, NY). Seven‐ to 9‐wk‐old mice were used throughout the experiments. We have not observed significant differences in the bacterial clearance of male and female mice to ocular P. aeruginosa‐induced infection.
Kugadas A., Geddes‐McAlister J., Guy E., DiGiandomenico A., Sykes D.B., Mansour M.K., Mirchev R, & Gadjeva M. (2019). Frontline Science: Employing enzymatic treatment options for management of ocular biofilm‐based infections. Journal of Leukocyte Biology, 105(6), 1099-1110.
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4

Murine Tissue Acquisition for Research

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C57BL/6J and DBA/2J mice were purchased from Charles River Laboratories (Wilmington, USA). Swiss Webster and 129S6/SvEvTac mice were purchased from Taconic Biosciences (Rensselaer, USA). All the mice were housed in the animal facility of ETH Zurich. All animal experiments were performed under the license ZH152/17 in accordance with the standards and regulations of the Cantonal Ethics Commission Zurich.
Aizawa E., Dumeau C.E., Freimann R., Di Minin G, & Wutz A. (2020). Polyploidy of semi-cloned embryos generated from parthenogenetic haploid embryonic stem cells. PLoS ONE, 15(9), e0233072.
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5

Microbiome Modulation in C57BL/6 Mice

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C57BL/6 mice were obtained from the Jackson Labs, Swiss-Webster, B10A and
BALB/c mice were obtained from Taconic Farms. Germ-free Swiss Webster mice were
obtained from Taconic farms Gnotobiotic Center. C57BL/6 germ-free mice were
obtained from the National Gnotobiotic Rodent Resource Center at the University
of North Carolina. The study was approved by the NIAID, NIH and Oregon State
University Animal Care and Use Committees. Mice used in the experiments were of
both sexes and 2–4 months old. Antibiotics were administered in drinking
water for 4–5 weeks in the following concentrations: ampicillin (1 g/L),
vancomycin (0.5 g/L), neomycin trisulfate (1 g/L), metronidazole (1 g/L). Mouse
small intestines (ileum) were either snap frozen for RNA isolations or processed
for epithelial cell isolations as described in online supplementary methods.
Morgun A., Dzutsev A., Dong X., Greer R.L., Sexton D.J., Ravel J., Schuster M., Hsiao W., Matzinger P, & Shulzhenko N. (2015). Uncovering effects of antibiotics on the host and microbiota using transkingdom gene networks. Gut, 64(11), 1732-1743.
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6

In Utero Electroporation of Mouse Embryos

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Embryonic day (E) 15.5 timed-pregnant female Swiss Webster (Taconic Biosciences) mice were deeply anaesthetized with 2% isoflurane. Uterine horns were exposed and periodically rinsed with warm sterile 1X PBS. Plasmid DNA mixture was injected into the lateral ventricle of one cerebral hemisphere of an embryo using a 32-gauge needle (Hamilton Company) attached to a 5 μl syringe (Hamilton Company). Final plasmid DNA concentration was 4.5 μg/μl in water (DNA mass ratio of pAAV-CAG-S1-GCaMP6f, pAAV-CAG-S3-ExRaiAKAR, and pAAV-CAG-mRuby3–6xFLAG at 1:2:2). Fast Green FCF dye (Millipore Sigma) was added to the DNA mixture to visualize the mixture during injection. Five voltage pulses (50 V, 50 ms duration, 1 Hz) were delivered two times using 5-mm round plate electrodes (Harvard Apparatus), with the cathode placed on top of the skull to target the hippocampus. Electroporated embryos were placed back into the dam, and allowed to mature for delivery.
Linghu C., Johnson S.L., Valdes P.A., Shemesh O.A., Park W.M., Park D., Piatkevich K.D., Wassie A.T., Liu Y., An B., Barnes S.A., Celiker O.T., Yao C.C., Yu C.C., Wang R., Adamala K.P., Bear M.F., Keating A.E, & Boyden E.S. (2020). Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics. Cell, 183(6), 1682-1698.e24.
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7

Xenograft Tumor Model in Mice

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Male athymic NCr nude, Swiss-Webster and BALB/C mice between 6 and 8 wks of age were purchased from Taconic (Hudson, NY). NOD-scid IL2Rgammanull mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Housing for these animals was maintained in a HEPA-filtrated environment within sterilized cages with 12 h light/12 h dark cycles. All animal procedures were conducted with approval of and in compliance with University of Kentucky Institutional Animal Care and Use Committee. The original patient CRC tumor (F0 generation) was divided and implanted into the flank of a NOD scid gamma mouse (The Jackson Laboratory; 005557) mouse. When the tumors grew to 1 cm3, each tumor (F1 generation) was resected, divided into 2-mm3 pieces and implanted into 5 mice (F2 generation).
For intravenous injection of CRC cells, athymic NCr nude or BALB/C mice were anesthetized with isoflurane (induction 4%, maintenance 2%). The viability of cells used for inoculation was > 95% as determined by Vi-CELL™ XR (Beckman Coulter). Gentle pressure was applied to the inoculation site until there was no visible sign of bleeding. Lung tissues were preserved for histological examination by fixation in 10% buffered formalin followed by paraffin embedding.
Rychahou P., Bae Y., Reichel D., Zaytseva Y.Y., Lee E.Y., Napier D., Weiss H.L., Roller N., Frohman H., Le A.T, & Evers B.M. (2018). Colorectal cancer lung metastasis treatment with polymer–drug nanoparticles. Journal of controlled release : official journal of the Controlled Release Society, 275, 85-91.
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8

Xenograft Tumor Model in Mice

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Male athymic NCr nude, Swiss-Webster and BALB/C mice between 6 and 8 wks of age were purchased from Taconic (Hudson, NY). NOD-scid IL2Rgammanull mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Housing for these animals was maintained in a HEPA-filtrated environment within sterilized cages with 12 h light/12 h dark cycles. All animal procedures were conducted with approval of and in compliance with University of Kentucky Institutional Animal Care and Use Committee. The original patient CRC tumor (F0 generation) was divided and implanted into the flank of a NOD scid gamma mouse (The Jackson Laboratory; 005557) mouse. When the tumors grew to 1 cm3, each tumor (F1 generation) was resected, divided into 2-mm3 pieces and implanted into 5 mice (F2 generation).
For intravenous injection of CRC cells, athymic NCr nude or BALB/C mice were anesthetized with isoflurane (induction 4%, maintenance 2%). The viability of cells used for inoculation was > 95% as determined by Vi-CELL™ XR (Beckman Coulter). Gentle pressure was applied to the inoculation site until there was no visible sign of bleeding. Lung tissues were preserved for histological examination by fixation in 10% buffered formalin followed by paraffin embedding.
Rychahou P., Bae Y., Reichel D., Zaytseva Y.Y., Lee E.Y., Napier D., Weiss H.L., Roller N., Frohman H., Le A.T, & Evers B.M. (2018). Colorectal cancer lung metastasis treatment with polymer–drug nanoparticles. Journal of controlled release : official journal of the Controlled Release Society, 275, 85-91.
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9

Postnatal Mouse Hippocampal Neuronal Culture

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Procedures at MIT involving animals were in accordance with the National Institutes of Health Guide for the care and use of laboratory animals and approved by the Massachusetts Institute of Technology Animal Care and Use Committee. Procedures at BU were approved by the Boston University Institutional Animal Care and Use Committee. Zebrafish experiments at Janelia were conducted according to protocols approved by the Institutional Animal Care and Use Committee of the Howard Hughes Medical Institute, Janelia Research Campus. Hippocampal neuron culture was prepared from postnatal day 0 or day 1 Swiss Webster (Taconic) mice as previously described (Klapoetke et al. 2014 (link)). In-utero electroporation was performed on female Swiss Webster mice (Taconic).
Shemesh O.A., Linghu C., Piatkevich K.D., Goodwin D., Celiker O.T., Gritton H.J., Romano M.F., Gao R., Yu C.C., Tseng H.A., Bensussen S., Narayan S., Yang C.T., Freifeld L., Siciliano C.A., Gupta I., Wang J., Pak N., Yoon Y.G., Ullmann J.F., Guner-Ataman B., Noamany H., Sheinkopf Z.R., Park W.M., Asano S., Keating A.E., Trimmer J.S., Reimer J., Tolias A.S., Bear M.F., Tye K.M., Han X., Ahrens M.B, & Boyden E.S. (2020). Precision calcium imaging of dense neural populations via a cell body-targeted calcium indicator. Neuron, 107(3), 470-486.e11.
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10

Mouse Genetic Tools for Neuroscience Research

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Animal handling and experimentation were performed in accordance with US National Institute of Health and Weill Cornell Medical College Institutional Animal Care and Use Commission. Animals of both sexes were used and housed in a controlled environment on a 12 h light/dark cycle with food and water ad libitum. Swiss Webster (Taconic), VIPCre (Jackson Laboratories, 010908), Lhx6.Cre (Jackson Laboratories, 026555), Emx1Cre (Jackson Laboratories, 005628), SERTCre (Jackson Laboratories, 014554), Wnt3aCre (a gift from Ulrich Müller), RCL-GCaMP6s (Ai96, Jackson Laboratories, 024106), RCL-ChR2 (Ai32, Jackson Laboratories, 024109), NR1fl (Jackson Laboratories, 005246), and 5HT3aR.Cre (a gift from N. Heintz, Rockefeller University), were used for the studies. 5HT3aR.Cre, VIPCre, Lhx6.Cre, Emx1Cre and SertCre were crossed with RCL-GCaMP6s (denoted as 5HT3aR.GCaMP6s, VIP.GCaMP6s, Lhx6.GCaMP6s, Emx1.GCaMP6s, Sert.GCaMP6s). 5HT3aR.Cre animals were crossed with NR1fl/fl (denoted as 5HT3aR.Cre,NRfl/fl). Information about mouse strains including genotyping protocols can be found at http://www.jax.org and elsewhere (Gorski et al., 2002 (link); Louvi et al., 2007 (link); Narboux-Nême et al., 2008 (link); Taniguchi et al., 2011 (link)).
Che A., Babij R., Iannone A.F., Fetcho R.N., Ferrer M., Liston C., Fishell G, & De Marco Garcia N.V. (2018). Layer I interneurons sharpen sensory maps during neonatal development. Neuron, 99(1), 98-116.e7.
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