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Hifi sequel 2 system with chemistry 2

Manufactured by Pacific Biosciences
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The HiFi Sequel II System with Chemistry 2.0 is a long-read sequencing platform developed by Pacific Biosciences. It is designed to generate high-fidelity (HiFi) sequencing data with an average read length of over 20 kilobases. The system utilizes the company's proprietary SMRT (Single Molecule, Real-Time) technology to provide a comprehensive solution for complex genomic analysis and de novo genome assembly.

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Sequel II System Sequel system Sequel II

2 protocols using hifi sequel 2 system with chemistry 2

1

Haplotype-resolved assembly of HG002 genome

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We use the haplotype-resolved assembly produced by hifiasm v0.11 using 34x coverage (two 15 kb and two 20 kb libraries) by PacBio HiFi Sequel II System with Chemistry 2.0 reads (https://github.com/human-pangenomics/HG002_Data_Freeze_v1.0#hg002-data-freeze-v10-recommended-downsampled-data-mix) using kmer information from parental Illumina short reads (30× 2×150bp reads at https://s3-us-west-2.amazonaws.com/human-pangenomics/index.html?prefix=NHGRI_UCSC_panel/HG002/hpp_HG002_NA24385_son_v1/parents/ILMN/downsampled/), described recently2 (link).
Wagner J., Olson N.D., Harris L., McDaniel J., Cheng H., Fungtammasan A., Hwang Y.C., Gupta R., Wenger A.M., Rowell W.J., Khan Z.M., Farek J., Zhu Y., Pisupati A., Mahmoud M., Xiao C., Yoo B., Sahraeian S.M., Miller D.E., Jáspez D., Lorenzo-Salazar J.M., Muñoz-Barrera A., Rubio-Rodríguez L.A., Flores C., Narzisi G., Evani U.S., Clarke W.E., Lee J., Mason C.E., Lincoln S.E., Miga K.H., Ebbert M.T., Shumate A., Li H., Chin C.S., Zook J.M, & Sedlazeck F.J. (2022). Curated variation benchmarks for challenging medically-relevant autosomal genes. Nature biotechnology, 40(5), 672-680.
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2

Haplotype-resolved assembly of HG002 genome

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We use the haplotype-resolved assembly produced by hifiasm v0.11 using 34x coverage (two 15 kb and two 20 kb libraries) by PacBio HiFi Sequel II System with Chemistry 2.0 reads (https://github.com/human-pangenomics/HG002_Data_Freeze_v1.0#hg002-data-freeze-v10-recommended-downsampled-data-mix) using kmer information from parental Illumina short reads (30× 2×150bp reads at https://s3-us-west-2.amazonaws.com/human-pangenomics/index.html?prefix=NHGRI_UCSC_panel/HG002/hpp_HG002_NA24385_son_v1/parents/ILMN/downsampled/), described recently2 (link).
Wagner J., Olson N.D., Harris L., McDaniel J., Cheng H., Fungtammasan A., Hwang Y.C., Gupta R., Wenger A.M., Rowell W.J., Khan Z.M., Farek J., Zhu Y., Pisupati A., Mahmoud M., Xiao C., Yoo B., Sahraeian S.M., Miller D.E., Jáspez D., Lorenzo-Salazar J.M., Muñoz-Barrera A., Rubio-Rodríguez L.A., Flores C., Narzisi G., Evani U.S., Clarke W.E., Lee J., Mason C.E., Lincoln S.E., Miga K.H., Ebbert M.T., Shumate A., Li H., Chin C.S., Zook J.M, & Sedlazeck F.J. (2022). Curated variation benchmarks for challenging medically-relevant autosomal genes. Nature biotechnology, 40(5), 672-680.
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