Apoptosis was detected using the Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit (Beyotime, Shanghai, China). Briefly, cells were collected with trypsin/ethylenediaminetetraacetic acid and resuspended in binding buffer. Next, cells were cultured with 5 μL Annexin V-FITC at 4°C for 15 min and with 5 µL PI at 4°C for 5 min. After incubation, apoptotic cells were quantified by a flow cytometer (BD Biosciences, San Jose, CA, USA), and data analysis was implemented using BD AccuriTM C6 software.
Annexin 5 fluorescein
Manufactured by Beyotime
Sourced in China
Annexin V-fluorescein is a protein that binds to phosphatidylserine, a phospholipid found on the surface of apoptotic cells. It is commonly used as a fluorescent marker in flow cytometry and microscopy applications to detect and quantify cells undergoing programmed cell death or apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Pi apoptosis detection kit, by Beyotime (2 mentions)
Facscan flow cytometry, by BD (2 mentions)
Fitc propidium iodide apoptosis detection kit, by Beyotime (2 mentions)
Accuri c6, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Facscalibur flow, by BD (1 mentions)
Flow cytometry, by BD (1 mentions)
Phosphate buffered saline (pbs), by Beyotime (1 mentions)
Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions)
Flow cytometry, by Beckman Coulter (1 mentions)
11 protocols using annexin 5 fluorescein
1
Apoptosis Detection Using Annexin V-FITC/PI
2
Annexin V-FITC and PI Apoptosis Assay
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To examine apoptosis, cells were double-stained with an Annexin V-Fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection kit (Beyotime Institute of Biotechnology) according to the manufacturer’s protocol. After Prucalopride treatments for 48 h, the cells were harvested, washed with cold phosphate-buffered saline (PBS), resuspended with binding buffer. Then cells were incubated with 5 μl Annexin-V-FITC reagent under room temperature for 5 min and were stained by PI reagent. The percentages of apoptosis cells were then analyzed by FACS Calibur flow cytofluorometry (BD Biosciences, San Jose, CA, USA) for the early apoptotic (Annexin V+ and PI−) and late apoptotic (Annexin V+ and PI+) cells. The apoptotic rate was determined using CellQuest software (BD Biosciences).
3
Evaluating HCC38 Cell Apoptosis by Flow Cytometry
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HCC38 cell apoptosis was measured in the four groups by FCM, using an Annexin V-fluorescein isothiocyanate (V-FITC) apoptosis detection kit (Beyotime Institute of Biotechnology). HCC38 cells were cultured, collected and washed with cold PBS twice, and subsequently treated with puerarin for 48 h at 37°C. Cells were treated with Annexin propidium iodide and V-FITC. Subsequently the cells were centrifuged at 100 × g at 25°C for 5 min. Cell apoptosis was measured using a flow cytometer and FlowJo software (version 10; FlowJo LLC, Ashland, OR, USA).
4
Apoptosis Assessment of Chemoresistant Prostate Cancer Cells
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The apoptosis of PC-3/R and DU145/R cells was assessed using an annexin V-fluorescein isothiocyanate (FITC) apoptosis-detection kit (Beyotime). Briefly, transfected PC-3/R and DU145/R cells were cultured with PTX (30 nM) for 48 h. After washing, the cells were re-suspended in binding solution with a concentration of 106 cells/mL. Then, annexin V-FITC (10 μL) and propidiumiodide (5 μL) were added to the binding solution with PC-3/R and DU145/R cells and incubated for 20 min in the dark. In the end, the apoptosis rate of PC-3/R and DU145/R cells was determined using the FACScan® flow cytometry (BD Biosciences).
5
Apoptosis Quantification in SMMC-7721 Cells
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At 48 h post-transfection, SMMC-7721 cells were suspended in binding buffer and were hatched with an Annexin-V-fluorescein isothiocyanate (FITC)/PI apoptosis detection kit (Beyotime Institute of Biotechnology) for 30 min at room temperature in the dark. Flow cytometry was performed using a flow cytometer. The results were analyzed using FlowJo software (version 7.6; FlowJo LLC, Ashland, OR, USA).
6
Apoptosis Evaluation in Drug-Resistant Cells
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After relevant transfection, the apoptosis of TE1/DDP and KYSE410/DDP cells was evaluated through the Annexin V‐fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit (Beyotime). Briefly, transfected cells were harvested, washed, and resuspended. Then, 5 μl Annexin V‐FITC and 5 μl PI were added and maintained for 15 min in the dark to stain cells. The apoptotic cells were analyzed with a flow cytometry (BD Biosciences).
7
Annexin V-FITC/PI Apoptosis Assay
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CHON-001 cell apoptosis was assessed with Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit (Beyotime) according to the manufacturers’ guidelines. Briefly, CHON-001 cells were collected and rinsed with PBS (Beyotime) after relevant treatment and then resuspended in binding buffer. After that, 5 μL AnnexinV-FITC and 5 μL PI were added and maintained for 15 min in the dark. At last, cell apoptosis was examined with a FACScan® flow cytometry (BD Biosciences, San Jose, CA, USA).
8
Annexin V-FITC Apoptosis Assay in AML12 Cells
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After different treatments, the AML12 cells were detached and centrifuged with supernatant removed. Then, AML12 cell apoptosis was assayed with the Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) kit (C1062L, Beyotime) with an Attune NxT flow cytometer (Thermo Fisher Science).
9
Annexin V-FITC/PI Apoptosis Assay
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The apoptosis of GC cells was assessed using the Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit (Beyotime). After being transfected for 48 h, cells were collected, washed and resuspended at a concentration of 1.0 × 106 cells/mL. Then 100 μL cells were added into the tube. After that, 5 μL Annexin V-FITC and 5 μL PI were added into the tube in the dark for 15 min. Next, 400 μL binding buffer was added and mixed. Finally, FACScan® flow cytometry (BD Biosciences, San Jose, CA, USA) was used to detect the stained cells within 1 h.
10
Quantifying Apoptosis in Colorectal Cancer
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The apoptosis of HCT-116 and SW480 cells was detected using the Annexin V fluorescein isothiocynate (FITC)/propidium iodide (PI) apoptosis detection kit (Beyotime). The 6-well plates with transfected CRC cells were placed in a 37°C incubator for 48 h. Next, cells were harvested, FITC and PI were applied to stain the cells for 20 min in the absence of light, and the cells were analyzed by flow cytometry (FlowCam, Shanghai, China).
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