Soil, root, and wood samples were randomly collected from different regions of eastern forests in Guilan Province, Northern Iran during 2015. Nematodes were recovered directly from the wood samples by the Whitehead tray method (Whitehead and Hemming, 1965 ). The extracted nematodes were observed and handpicked under a stereomicroscope. Adult specimens for microscopic observation were killed with gentle heat and fixed in a solution of FGA 4:1:1 (formaldehyde, glycerin, and acetic acid) and then processed to anhydrous glycerin (De Grisse, 1969 ). Permanent slides were made and examined with a Nikon E200 light microscope. Morphometric data were obtained with the aid of a drawing tube attached to an Olympus BH2 light microscope. Photomicrographs were taken with a digital camera attached to an Olympus BH2 light microscope. Line drawings were made and photographs were taken with a digital camera attached to an Olympus BH2 microscope.
Bh 2 light microscope
Manufactured by Olympus
Sourced in Japan, United States
The BH-2 is a light microscope designed for laboratory use. It provides magnification and illumination to observe specimens. The core function of the BH-2 is to facilitate the visual examination of small-scale samples and materials.
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Lab products found in correlation
Mayer s h e, by Yeasen (3 mentions)
Bh 2 microscope, by Olympus (1 mentions)
Progresc7 camera, by Jenoptik (1 mentions)
Dab substrate, by Merck Group (1 mentions)
S 4800 scanning electron microscope, by Hitachi (1 mentions)
Rm2065 microtome, by Leica (1 mentions)
Cm3050s cryostat, by Leica (1 mentions)
Compound light microscope, by Olympus (1 mentions)
Nis elements 3, by Nikon (1 mentions)
Goat serum, by Thermo Fisher Scientific (1 mentions)
Ab34710, by Abcam (1 mentions)
Ab36861, by Abcam (1 mentions)
Ab26414, by Abcam (1 mentions)
Ab75606, by Abcam (1 mentions)
Ab39634, by Abcam (1 mentions)
Ab76956, by Abcam (1 mentions)
Ab38898, by Abcam (1 mentions)
Xylene, by Merck Group (1 mentions)
Phosphotungstic acid, by Merck Group (1 mentions)
Azon red anilin blue, by Merck Group (1 mentions)
84 protocols using bh 2 light microscope
1
Nematode Identification from Eastern Iranian Forests
2
Microscopy Imaging Workflow for Research
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Optical microscope images were acquired using a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan) attached to an AmScope 1000 video camera (United Scope LLC, dba AmScope, Irvine, CA, USA), linked to an ASUS laptop equipped with HDMI high-definition multimedia interface system (Taiwan–USA, Fremont, CA, USA). Images from the microscope are transferred from the laptop to a USB and stored for subsequent processing on a computer.
3
Histological Analysis of Infected Intestine in L. belcheri
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For the histological sections, heavily infected intestinal tissues of L. belcheri collected from Playa Pasamayo, Ancón, Lima in 1981 were fixed in 10% buffered formalin. After dehydration and embedding in paraffin, the specimens were processed using standard methods comparable to those of Kiernan [43 ] and Bancroft and Gamble [22 ]. These paraffin tissue blocks were sectioned at 4–6 microns, placed on glass slides and stained with hematoxylin and eosin (HE). The prepared glass slides were viewed with a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan); see Microscope Images above.
4
Worm Staining and Microscopic Imaging Protocol
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Worms were punctured with a fine needle and subsequently stained in Mayer’s acid carmine, destained in 4% hydrochloric acid in 70% ethanol, dehydrated in ascending concentrations of ethanol (24 h each), and cleared in 100% xylene then in 50% Canada balsam and 50% xylene (24 h each). Whole worms were then mounted in Canada balsam. Measurements are in micrometers, unless otherwise noted; the range is followed by the mean values between parentheses. Width measurements represent maximum width. Trunk length does not include proboscis, neck, or bursa.
Microscope images were created using 10X and 40X objective lenses of a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan) attached to an AmScope 1000 video camera (United Scope LLC, dba AmScope, Irvine, CA, USA), linked to an ASUS labtop equipped with an HDMI high definition multimedia interface system (Taiwan–USA, Fremont, CA, USA). Images from the microscope were transferred from the labtop to a USB and stored for subsequent processing on a computer.
Microscope images were created using 10X and 40X objective lenses of a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan) attached to an AmScope 1000 video camera (United Scope LLC, dba AmScope, Irvine, CA, USA), linked to an ASUS labtop equipped with an HDMI high definition multimedia interface system (Taiwan–USA, Fremont, CA, USA). Images from the microscope were transferred from the labtop to a USB and stored for subsequent processing on a computer.
5
Optical Microscope Image Acquisition
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Optical microscope images were acquired using a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan) attached to an AmScope 1000 video camera (United Scope LLC, dba AmScope, Irvine, CA, USA), linked to an ASUS laptop.
6
Staining and Visualizing Worm Specimens
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Worms were punctured with a fine needle and subsequently stained in Mayer's acid carmine, destained in 4% hydrochloric acid in 70% ethanol, dehydrated in ascending concentrations of ethanol (24 h each) and cleared in 100% xylene then in 50% Canada balsam and 50% xylene (24 h each). Whole worms were then mounted in Canada balsam. Measurements are in micrometres, unless otherwise noted; the range is followed by the mean values between parentheses. Width measurements represent Microscope images were created using 10× and 40× objective lenses of a BH2 light Olympus microscope (Olympus Optical Co., Osachi-shibamiya, Okaya, Nagano, Japan) attached to an AmScope 1000 video camera (United Scope LLC, dba AmScope, Irvine, California, USA), linked to an ASUS laptop equipped with a high-definition multimedia interface system (Fremont, California, USA). Images from the microscope are transferred from the laptop to a USB and stored for subsequent processing on a computer.
Specimens were deposited in the University of Nebraska State Museum's Harold W. Manter Laboratory (HWML) under collection number 139,404 (voucher specimens on one slide), Lincoln, Nebraska, USA.
Specimens were deposited in the University of Nebraska State Museum's Harold W. Manter Laboratory (HWML) under collection number 139,404 (voucher specimens on one slide), Lincoln, Nebraska, USA.
7
Optical Microscope Image Acquisition Workflow
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Optical microscope images were acquired using a BH2 light Olympus microscope (Olympus Optical Co., Okaya, Nagano, Japan) attached to an AmScope 1,000 video camera (United Scope LLC, dba AmScope, Irvine, California), linked to an ASUS laptop equipped with HDMI high-definition multimedia interface system (Taiwan-USA, Fremont, California). Images from the microscope were transferred from the laptop to a USB and stored for subsequent processing on a computer.
8
Comprehensive Revision of Syntrichia Genus
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For morphological delimitation, taxonomic conclusions and characterization as part of the work to develop a worldwide revision of Syntrichia, we studied specimens from these herbaria: B, BA, BM, BOLUS, BR, CANM, CAS, COLO, CU, DUKE, E, EGR, F, FH, FI, FLAS, FT, GB, H, JE, L, LIL, LPB, M, MA, MEXU, MICH, MO, MUB, NMW, NY, O, PC, PRE, S, SGO, SP, U, UPS, US, W, and Z, as well as material collected in the field by the authors [25 ]. Additionally, most of the type material of the taxa currently attributed to Syntrichia from throughout the world was studied. The morphological study of the allied genera has been complemented by the examination of most of their original material. We used the conventional anatomical and morphological methods applied for the Pottiaceae [3 ]. Microscopic examinations and measurements were taken with an Olympus-BH2 light microscope, while microphotographs were obtained with a Jenoptik ProgRes C7 camera mounted on this microscope.
9
Avian Haemosporidian Parasite Screening
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Blood samples were collected of pets, domestic and wild injured birds referred to the veterinary clinic during October 2018 to September 2019 and some blood samples were collected by a veterinarian during visiting hosts in farms. Brachial vein blood was taken with a syringe (50–100 μL of whole blood). Two thin blood smears were prepared for each host, were immediately dried in the air, preserved in absolute methanol and subsequently stained with Giemsa 10% at pH 7.2 (60 minutes) for the morphological screening of haemosporidian parasites. Blood films were examined under 400× and 1,000× of the Olympus BH2 light microscope (Olympus Co, Japan) via oil immersion in 100 fields for 35–40 minutes for haemosporidian parasites gametocytes discovery (Martinsen et al., 2006 ; Valkiūnas, 2005 ; Valkiūnas & Iezhova, 2018 ). Blood samples were immediately stored in an anticoagulant buffer and subsequently stored at −20°C until molecular experiments. More information about the bird's health condition was registered in agreement with the history from their owners or finders (Table 1 ).
10
Histopathological Analysis of Organ Tissues
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Lung, brain, heart, and kidney tissues taken from the subjects were fixed in 10% neutral formalin. Sample tissues were submerged in paraffin after being put through degreed alcohol and xylene. 5-μm-thick sections were taken and dyed with hematoxylin eosin and toluidine blue for general examination and for obtaining the mast cell count, respectively; and they were assessed using a BH2 Olympus light microscope. Histopathological evaluations were performed on each subject’s lung, brain, heart, and kidney sample sections. Changes in the lung tissue were scored in terms of alveolar edema, hemorrhage, cell infiltration, and alveolar wall thickness. Scoring was assigned as normal structure (0), minor changes (1), moderate changes (2), and widespread changes (3) [15 (link)]. In the lung tissue of all subjects, the mast cell count was obtained at 40× scope magnification in 6 different areas. Histopathological scoring was performed by a researcher who was not informed about the subject groups.
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