1.5 t avanto

Total serum bilirubin (TSB) measurements were recorded as close as possible to 6, 12, 24, 48, 72, 96, 120, 132, 144, 156 and 168 h of age for each infant. Blood samples (50 µl) were collected to routinely measure bilirubin levels in our clinical biochemistry laboratory using a spectrophotometer or from a bedside blood gas analysis. Samples were collected beginning on day 1 of life and daily thereafter as long as an indwelling catheter was in place (5–7 days); in a few instances, transcutaneous bilirubin (TCB) results were used instead. The means and 95% confidence intervals were calculated for each defined time within each group during the first 7 days of life. These values were plotted for infants with varying severities of HIE and for controls as well. Clinical MRI scans were routinely performed on either a Siemens 1.5-T Avanto or 3.0-T Trio (Siemens Medical, Erlangen, Germany) scanner according to our MRI protocol for infants with HIE, and the results were assessed using the MRI scoring system developed by Barkovich et al. [9 (link)]. More extensive injuries resulted in higher scores.

All MRI measurements were made on Siemens 1.5 T Avanto scanners (Siemens Medical Systems, Erlangen, Germany) at Fremantle Hospital, St John of God Murdoch Hospital, and Hollywood Private Hospital, Western Australia. The median time between biopsy and MRI was 57 days. Data were acquired as prescribed by the HepaFat-Scan^® methodology (Resonance Health Ltd, Perth, Australia). Phased-array torso coils were centred over the liver of the subjects. MRI data acquisition comprised an opposed-phase, in-phase, opposed-phase gradient echo sequence (TEs 2.38, 4.76, 7.14 ms, TR 88 ms, 1 excitation, flip angle 70 degrees, bandwidth 500 Hz). Data from three axial slices, positioned through the widest part of the liver, were acquired in a single breath-hold. The slice thickness was 4 mm and the matrix was 256 x 256 with a field of view 300 x 300 mm. Liver iron concentrations (LIC) were measured using a validated non-invasive MRI method (FerriScan^®) [45 (link), 46 (link)].