Anti girdin

Manufactured by R&D Systems

Anti-Girdin is a recombinant protein that binds to the actin-binding protein Girdin. It is used in research applications to study the role of Girdin in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti girdin

Comprehensive Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following Abs were used in this study: anti‐Girdin (R&D Systems), anti‐Girdin (IBL), anti‐Girdin phospho S1647 (ECM Biosciences), anti‐histone H3 (1B1B2) (Cell Signaling Technology), anti‐histone H3 phospho S10 (Abcam), anti‐histone H3 phospho S28 (Abcam), anti‐cleaved PARP1 (Abcam), anti‐cleaved PARP1 (Cell Signaling Technology), anti‐Rb phospho Ser795 (New England BioLabs), anti‐Rb (4H1) (Cell Signaling Technology), anti‐p53 (Cell Signaling Technology), anti‐p53 phospho S15 (Cell Signaling Technology), anti‐p53 phospho S46 (Cell Signaling Technology), anti‐Mad2 (C‐10) (Santa Cruz Biotechnology), anti‐α‐tubulin (Sigma‐Aldrich), anti‐γ‐tubulin (Sigma‐Aldrich), Alexa Fluor 488 goat anti‐mouse IgG (Thermo Fisher Scientific), Alexa Fluor 488 goat anti‐rabbit IgG (Thermo Fisher Scientific), rabbit anti‐sheep IgG (H + L), Human SP ads‐HRP (Southern Biotech), and rabbit anti‐rat IgG H&L (HRP) (Abcam) Abs.

Chen C., Enomoto A., Weng L., Taki T., Shiraki Y., Mii S., Ichihara R., Kanda M., Koike M., Kodera Y, & Takahashi M. (2020). Complex roles of the actin‐binding protein Girdin/GIV in DNA damage‐induced apoptosis of cancer cells. Cancer Science, 111(11), 4303-4317.

+ Open protocol

+ Expand

Protein Immunodetection and Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: anti-Girdin (R&D Systems, Minneapolis, MN, #AF5345, for WB and IP), anti-4F2hc (H300, Santa Cruz Biotechnology, Santa Cruz, CA, for WB, clone MEM-108, Biolegend [#315602] for IP, and mouse monoclonal anti-4F2hc [clone HBJ 127] for IF), anti-glutathione S-transferase (GST) (Santa Cruz Biotechnology, #sc-459), anti-Myc (clone 9E10, Santa Cruz Biotechnology), anti-poly-histidine (clone HIS-1, Sigma, St. Louis, MO), anti-Flag (clone M2, Sigma), anti-β-actin (clone AC-74, Sigma), normal mouse IgG (Millipore, Milford, MA, #12–371), and normal sheep IgG (Millipore, #12–515). Antibodies to pS6K (Thr389) (#9205), S6K (#9202), S6 (#2217), pS6 (Ser240/244) (#2215), MAPK (#9102), pMAPK (Thr202/Tyr204) (#9106), Lamp1 (#9109), mTOR (#2983), and LC3B (#2775) were purchased from Cell Signaling Technology (Danvers, MA). Flag M2 affinity gel, adenosine 5′-triphosphate (ATP), and amino acids were purchased from Sigma. Phos-tag Acrylamide (Wako, Saitama, Japan) was used for the generation of the Phos-tag gel to analyze protein phosphorylation in cells.

Weng L., Han Y.P., Enomoto A., Kitaura Y., Nagamori S., Kanai Y., Asai N., An J., Takagishi M., Asai M., Mii S., Masuko T., Shimomura Y, & Takahashi M. (2018). Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex. PLoS Biology, 16(3), e2005090.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!