The images were captured using confocal microscopes. Notably, epifluorescent microscopes can also be successfully used to create high quality images suitable for the segmentation pipeline (not shown here, see Lignell et al., 2017 (link)). All embryonic mouse kidney samples were mounted in 99.5% glycerol on glass slides with coverslip spacers (Invitrogen) and imaged using a Leica TCS SP8 X confocal microscope (63x oil-immersion objective, NA 1.4). The chick neural tube images were acquired by using the Andor Dragonfly Spinning disc confocal microscope (63x water immersion NA 1.7). The epithelial spheroids were imaged by using a Nikon A1R + confocal microscope (40x oil-immersion objective, NA 1.3.)
Coverslip spacers
Manufactured by Thermo Fisher Scientific
Coverslip spacers are thin, transparent materials used to maintain a specific distance between a microscope slide and its coverslip. They are designed to provide a consistent sample thickness, enabling optimal focus and imaging quality during microscopy applications.
Automatically generated - may contain errors
2 protocols using coverslip spacers
1
Confocal Microscopy for Imaging Embryonic Tissues
2
Confocal Microscopy for Imaging Embryonic Tissues
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The images were captured using confocal microscopes. Notably, epifluorescent microscopes can also be successfully used to create high quality images suitable for the segmentation pipeline (not shown here, see Lignell et al., 2017 (link)). All embryonic mouse kidney samples were mounted in 99.5% glycerol on glass slides with coverslip spacers (Invitrogen) and imaged using a Leica TCS SP8 X confocal microscope (63x oil-immersion objective, NA 1.4). The chick neural tube images were acquired by using the Andor Dragonfly Spinning disc confocal microscope (63x water immersion NA 1.7). The epithelial spheroids were imaged by using a Nikon A1R + confocal microscope (40x oil-immersion objective, NA 1.3.)
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