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Rag2 il 2rγ mice

Manufactured by Taconic Biosciences
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Sourced in Germany, United States

The Rag2−/−IL-2Rγ−/− mice are a genetically engineered mouse model developed by Taconic Biosciences. This model lacks the Rag2 and Il2rg genes, resulting in severe combined immunodeficiency (SCID) with the absence of T cells, B cells, and natural killer cells.

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Lab products found in correlation

C57bl 6 wt and, by Jackson ImmunoResearch (2 mentions) Rag1 mice, by Jackson ImmunoResearch (2 mentions) B6 sjl mice, by Taconic Biosciences (1 mentions) C57bl 6, by Taconic Biosciences (1 mentions) Rorc cre, by Jackson ImmunoResearch (1 mentions) R26r eyfp mice, by Jackson ImmunoResearch (1 mentions) C57bl 6 and, by Jackson ImmunoResearch (1 mentions) B6 sjl mice, by Jackson ImmunoResearch (1 mentions) Nsg mice, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

6 protocols using rag2 il 2rγ mice

1

Generating Genetically Modified Mice

Cited 2 times
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Mice were bred and maintained under specific pathogen-free conditions in accordance with animal facilities at the Seattle Children’s Research Institute (SCRI). Experiments were conducted in accordance with the guidelines of the SCRI Animal Care and Use Committee. IL-23R−/− and DOCK8-deficient (DOCK8pri/pri) mice were generated as previously described 24 (link), 45 (link). WT (C57BL/6) and Rag1−/− mice were purchased from Jackson Laboratories (Bar Harbor, ME) while Rag2−/−IL-2Rγ−/− mice were purchased from Taconic. Most of the animal used in the study were 6 to 8 weeks old male and female mice.
Singh A.K., Eken A., Fry M., Bettelli E, & Oukka M. (2014). DOCK8 regulates protective immunity by controlling the function and survival of RORγt+ ILCs. Nature communications, 5, 4603.
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2

Mouse Strain Generation and Maintenance

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C57BL/6 and B6.SJL mice were purchased from Taconic Biosciences (Germantown, NY). A breeding pair of Rag2−/−IL-2Rγ−/− mice was purchased from Taconic Biosciences, and these mice were subsequently bred in-house. Rorc.cre and R26R-EYFP mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Rorc.cre mice were bred with R26R-EYFP mice in-house to produce RORγt fate mapping mice. NFIL3−/− mice were a generous gift from Dr. Hugh JM Brady (15 (link)). NFIL3+/+, NFIL3+/−, and NFIL3−/− mice were subsequently bred in-house. All mice were maintained in pathogen free facilities at Brown University. Mice of both sexes were included and no differences were observed.
Erick T., Grigoryan L, & Brossay L. (2017). Lacrimal Gland NK Cells Are Developmentally and Functionally Similar to Conventional NK Cells. ImmunoHorizons, 1(2), 2-9.
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3

Murine Immune Cell Lineage Evaluation

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C57BL/6 and B6.SJL mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and Taconic Biosciences (Germantown, NY), respectively. Rag2−/−IL-2Rγ−/− mice were purchased from Taconic Biosciences and maintained in-house. Rag1−/−, B6;129P2(SJL)-Myd88tm1Defr/J (Myd88fl/fl), B6;129-NCR1tm10man/J (NCR1gfp), and B6(Cg)-Ifnar1tm1.1Ees/J (Ifnar1fl/fl) mice were purchased from The Jackson Laboratory. IL-12Rβ2fl/fl mice were generated at the Brown University Transgenic Facility. Both age and sex-matched mice (6 − 14 weeks) were used for this study. All experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Brown University, and were conducted in accordance with institutional guidelines for animal care. All mice were maintained at Brown University in AAALAC-accredited, pathogen-free facilities.
Dodard G., Tata A., Erick T.K., Jaime D., Shahjahan Miah S.M., Quatrini L., Escalière B., Ugolini S., Vivier E, & Brossay L. (2020). Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells. Cell reports, 32(1), 107855.
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4

Generating Genetically Modified Mice

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were bred and maintained under specific pathogen-free conditions in accordance with animal facilities at the Seattle Children’s Research Institute (SCRI). Experiments were conducted in accordance with the guidelines of the SCRI Animal Care and Use Committee. IL-23R−/− and DOCK8-deficient (DOCK8pri/pri) mice were generated as previously described 24 (link), 45 (link). WT (C57BL/6) and Rag1−/− mice were purchased from Jackson Laboratories (Bar Harbor, ME) while Rag2−/−IL-2Rγ−/− mice were purchased from Taconic. Most of the animal used in the study were 6 to 8 weeks old male and female mice.
Singh A.K., Eken A., Fry M., Bettelli E, & Oukka M. (2014). DOCK8 regulates protective immunity by controlling the function and survival of RORγt+ ILCs. Nature communications, 5, 4603.
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5

Transgenic Mouse Strains for Immunology

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In this study, female and male IL-4Rα/IL-5-deficient BALB/c, Rag2tm1FwaII2rgtm1WjlRAG2IL-2Rγ-deficient BALB/c and C57BL/6 (RAG2IL-2Rγ−/−), and NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NOD scid gamma, NSG) BALB/c mice were used. These mouse strains were provided by the Institute of Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn (UKB) in Germany. RAG2IL-2Rγ−/− mice were purchased from Taconic Biosciences Inc (Cologne, Germany) and the other two strains were bred at the IMMIP and UKB animal facilitates, but originally, IL-4Rα/IL-5−/− were a gift from Prof. Dr. Klaus Matthaei (Matthaei, Stem Cell & Gene Targeting Laboratory, ANU College of Medicine, Biology and Environment, Canberra, Australia) and NSG mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA). Mice were kept under SPF conditions following German animal protection laws and EU guidelines 2010/63/E4 and had access to food and water ad libitum. Mice were shipped in filter topped boxes to the Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon in agreement with the veterinary office in Bonn, Germany. Upon arrival, mice were housed in the animal facility of the Research Foundation for Tropical Diseases and the Environment, University of Buea under SPF conditions following Cameroonian animal protection laws.
Chunda V.C., Fombad F.F., Kien C.A., Ebai R., Esofi F., Ntuh A.N., Ouam E., Gandjui N.V., Ekanya R., Nietcho F., Nchang L.C., Magha C., Njouendou A.J., Enyong P., Hoerauf A., Wanji S, & Ritter M. (2024). Comparative development of human filariae Loa loa, Onchocerca volvulus and Mansonella perstans in immunocompromised mouse strains. Frontiers in tropical diseases, 5, 1293632.
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6

Murine Model of Immune Deficiency

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Wild type C57BL/6J mice were obtained from the Jackson Laboratory (Bar Habor, ME, USA). Rag2-/- and Rag2-/-Il2rγ-/- mice, on a C57Bl/6 background, were originally obtained from Taconic Biosciences (Troy, New York, USA). Male and female mice between 6 and 10 weeks of age, an average weight of 25 g, were used in this study. All mice were bred and housed in specific pathogen-free rooms within the Tulane University School of Medicine animal facilities, in accordance with the guidelines of the institution.
Munyonho F.T., Clark R.D., Lin D., Khatun M.S., Pungan D., Dai G, & Kolls J.K. (2023). Precision-cut lung slices as an ex vivo model to study Pneumocystis murina survival and antimicrobial susceptibility. mBio, 15(1), e01464-23.
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