Daptomycin

Manufactured by Selleck Chemicals

Sourced in United States, Cameroon, Germany

Daptomycin is a lipopeptide antibiotic used in the laboratory setting. It functions as an antibacterial agent, targeting and disrupting the cell membrane of certain gram-positive bacteria.

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Lab products found in correlation

6 protocols using daptomycin

Daptomycin Lipid Membrane Interactions

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Daptomycin was purchased from Selleckchem
(Munich, Germany) and from Haorui-Pharma Chem Inc. (Irvine, CA); 18:1
(Δ9-Cis) PC (DOPC), 18:1 (Δ9-Cis) PG (DOPG), 18:1 Liss
Rhod PE (Rh-PE), 18:1 cardiolipin, and 18:1 Lysyl PG (lysyl PG) were
purchased from Avanti Polar Lipids (Alabaster, AL). Texas Red sulfonyl
chloride (MW 625) was purchased from Invitrogen Probe (Grand Island,
NY). Fluorescence-labeled BODIPY-Daptomycin was a gift from Cubist
Pharmaceuticals, Inc. (Lexington, MA).

Chen Y.F., Sun T.L., Sun Y, & Huang H.W. (2014). Interaction of Daptomycin with Lipid Bilayers: A Lipid Extracting Effect. Biochemistry, 53(33), 5384-5392.

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Antibiotic Compounds Procurement Protocol

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Ciprofloxacin hydrochloride was purchased from MP Biomedicals (Santa Ana, CA, USA); erythromycin hydrochloride, vancomycin hydrochloride, ampicillin, ceftriaxone sodium, levofloxacin, imipenem monohydrate, gentamicin sulfate, tetracycline hydrochloride, colistin sulfate, penicillin G potassium, teicoplanin, linezolid, clindamycin hydrochloride, and metronidazole were purchased from Sigma-Aldrich (St Louis, MO, USA); meropenem was purchased from TCI (Portland, OR, USA); and daptomycin and fidaxomicin were purchased from Selleckchem (Houston, TX, USA).

D’Atanasio N., Capezzone de Joannon A., Di Sante L., Mangano G., Ombrato R., Vitiello M., Bartella C., Magarò G., Prati F., Milanese C., Vignaroli C., Di Giorgio F.P, & Tongiani S. (2020). Antibacterial activity of novel dual bacterial DNA type II topoisomerase inhibitors. PLoS ONE, 15(2), e0228509.

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Daptomycin Encapsulation in Vacuoles

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In this study, daptomycin was purchased from Selleck Chemicals (Seoul, South Korea). Various concentrations of daptomycin (50, 100, 200 μg/mL) were mixed with 30% vacuoles, and the final volume was adjusted to 1 mL using PS buffer (10 mM PIPES KOH [pH 6.8], 200 mM sorbitol). Encapsulation was achieved via slow mixing overnight at 4°C. The unloaded drug was removed via centrifugation at 20,000 × g for 30 min, and the pellet was then washed five times with PS buffer and concentrated to 1 mL. The drug-encapsulating efficiency (DEE) was calculated using the following equation (29 (link)):

DEE (%) = \frac{Input antibiotic - Remaining antibiotic}{Input antibiotic} \times 100

The daptomycin concentration was read on a UV-visible (UV-vis) spectrophotometer (Mecasys, Daejeon, South Korea) by absorbance that was measured at 220.5 nm (28 (link)).

Lee J., Nguyen N.T., Tran L.M., Kim Y.H, & Min J. (2023). Targeted Killing of Staphylococcus aureus Using Specific Peptides Displayed on Yeast Vacuoles. Microbiology Spectrum, 11(3), e00920-23.

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Antibiotic-Resistant Bacterial Strains

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Vancomycin-sensitive and Vancomycin-resistant Enterococcus faecalis strains and methicillin-resistant S. aureus (MRSA) strains included clinical isolates collected at the university hospital UZ Leuven (Belgium). The Vancomycin-sensitive S. aureus strain was ATCC 6538 (Rosenbach). The Vancomycine-intermediate S. aureus (VISA) HIP5827 (Tenover et al., 1998 (link)) and Vancomycin-resistant S. aureus (VRSA) were kindly donated by Olivier Denis (Université Libre de Bruxelles, Belgium). E. faecalis and S. aureus strains were grown at 37°C on brain heart infusion (BHI; Becton-Dickinson, Erembodegem, Belgium) agar and lysogeny broth (LB; also known as Luria-Bertani medium) agar plates, respectively. C. difficile strain ATJ ribotype 014/20 and strain AIU ribotype 027 were kept on brain heart infusion agar plates, grown at 37°C in an anaerobic cabinet. Vancomycin, daptomycin, tigeglycine, and linezolid were purchased from Selleck Chemicals (Munich, Germany).

Mishra N.M., Stolarzewicz I., Cannaerts D., Schuermans J., Lavigne R., Looz Y., Landuyt B., Schoofs L., Schols D., Paeshuyse J., Hickenbotham P., Clokie M., Luyten W., Van der Eycken E.V, & Briers Y. (2018). Iterative Chemical Engineering of Vancomycin Leads to Novel Vancomycin Analogs With a High in Vitro Therapeutic Index. Frontiers in Microbiology, 9, 1175.

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In Vitro and In Vivo Evaluation of XF-73

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XF-73 was provided by Destiny Pharma plc (UK). Mupirocin, fusidine, retapamulin, vancomycin, erythromycin, linezolid, and daptomycin were purchased from Selleck. Test compounds were prepared in dimethyl sulfoxide (DMSO) (Sigma) stock solutions at a concentration of 12.8 mg/mL. To determine the minimum inhibitory concentration (MIC) of Mupirocin against the high-level Mupirocin-resistant strain, a second stock concentration of 1.6384 g/mL was prepared. For the in vivo animal experiments, XF-73 was obtained from Destiny Pharma Plc (UK) supplied as a dermal formulation designed for skin application and stored in a dark vial. Mupirocin ointment was obtained as a commercial preparation (Sino-US Tianjin SmithKline Pharmaceutical Co., Ltd.). The control group was topically administered with an equivalent volume of water, (control group).

Zhang C., Li J., Lu R., Wang S., Fu Z, & Yao Z. (2023). Efficacy of a Novel Antibacterial Agent Exeporfinium Chloride, (XF-73), Against Antibiotic-Resistant Bacteria in Mouse Superficial Skin Infection Models. Infection and Drug Resistance, 16, 4867-4879.

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MHC II Allele-Optimized PBMC Isolation

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Tryptic soy broth (TSB) was purchased from Research Products International. BBL Mueller Hinton II Broth Cation-Adjusted (caMHIIB) was purchased from Becton, Dickinson and Company (BD). Bovine serum albumin (BSA) and human serum (from human male AB plasma, U.S. origin, sterile-filtered) were purchased from Sigma-Aldrich. Ninety-six–well plates with lids, sterile, Greiner Bio-One, were purchased from VWR International. Daptomycin was purchased from Selleck Chemicals. Goat anti-mouse horseradish peroxidase–conjugated IgG antibody was from Thermo Fisher Scientific. Interleukin-2 (IL-2) ELISpot (enzyme-linked immunospot) kits were from Mabtech, and IL-2 was from PeproTech. Human donor PBMCs were purchased from Cellular Technology Limited and were chosen to represent MHC II genotypes that both (i) included MHC II alleles for which the designs were explicitly optimized and (ii) excluded MHC II alleles for which the designs were explicitly optimized. The sample proportion and U.S. population proportion [taken from (51 (link))] of the selected donors’ DRB1 MHC II alleles are shown in fig. S2.

Zhao H., Brooks S.A., Eszterhas S., Heim S., Li L., Xiong Y.Q., Fang Y., Kirsch J.R., Verma D., Bailey-Kellogg C, & Griswold K.E. (2020). Globally deimmunized lysostaphin evades human immune surveillance and enables highly efficacious repeat dosing. Science Advances, 6(36), eabb9011.

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