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Sierh 3

Manufactured by Horizon Discovery
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The SiERH-3 is a laboratory equipment designed for genetic manipulation and cell engineering applications. It is capable of performing precise CRISPR-based genome editing tasks. The core function of the SiERH-3 is to enable efficient and reliable gene insertion, deletion, or modification within target cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Control sirna, by Qiagen (1 mentions) Lipofetamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

2 protocols using sierh 3

1

siRNA Knockdown of ERH in HCC Cells

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siERH-3 and siERH-5 were obtained from Dharmacon (Lafayette, CO) and Qiagen (Venlo, Netherlands), respectively. Control siRNA was from Qiagen. Sequence for the siRNAs were as followed: siERH #3 GAACTTATGCTGACTACGA and siERH #5 GAGGATCTTGTTCAATCGGAA. Transfection of siRNA into HCC cells was performed using Lipofetamine RNAiMAX (Invitrogen, Carlsbad, CA) following manufacturer's instruction at final concentration of 10 nM.
Weng M.T., Tung T.H., Lee J.H., Wei S.C., Lin H.L., Huang Y.J., Wong J.M., Luo J, & Sheu J.C. (2015). Enhancer of rudimentary homolog regulates DNA damage response in hepatocellular carcinoma. Scientific Reports, 5, 9357.
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2

siRNA Knockdown of ERH Genes

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Two different siRNA oligonucleotide duplexes targeting ERH genes (designated siERH-3 and siERH-4) were purchased from Dharmacon. The sequences were 5'-UCAGUCAGUUFUUUFAUUU-3' (siERH-3) and 5'-GAACUUAUGCUGACUACGA-3' (siERH-4). Transient transfection of each siRNA duplex was performed using the LipofectamineTM 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) transfection reagent according to the manufacturer’s instructions.
Park J.H., Park M., Park S.Y., Lee Y.J., Hong S.C., Jung E.J., Ju Y.T., Jeong C.Y., Kim J.Y., Ko G.H., Hah Y.S, & Jeong S.H. (2020). ERH overexpression is associated with decreased cell migration and invasion and a good prognosis in gastric cancer. Translational Cancer Research, 9(9), 5281-5291.
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