Antibodies directed against mouse CD31 (PECAM-1) (affinity purified SL-4), raised in rabbit and purified as described elsewhere 20 (link). Rat monoclonal antibodies against mouse CD31 [clone: MEC13.3] (Cat No. 550274), CD45 [30-F11] (Cat No. 550539), CD11b [M1/70] (Cat No. 550282), Mac3 [M3/84] (Cat No. 550292), and VE-cadherin (VEC) [11D4.1] (Cat No. 550548) were purchased from BD Biosciences (San Jose, CA, USA). Rabbit polyclonal antibodies against Ki67 (Cat No. ab15580) were purchased from Abcam (Cambridge, MA, USA). Rabbit monoclonal antibodies against Survivin [71G4B7] (Cat No. #2808), YAP [D24E4] (Cat No. #8418), and phospho-YAP (Ser127) [D9W2I] (Cat No. #13008) (P-YAP) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). A mouse monoclonal antibody against glucose transporter 1 (GLUT-1) [SPM498] (Cat No. ab40084) was purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal antibodies against human CD31 [JC70A] (Cat No. M0823), a CD45 [2B11+PD7/26] (Cat No. M0701) and a monoclonal mouse anti human CD68 were purchased from DAKO. Rabbit polyclonal antibodies against human Ajuba (Cat No. HPA006171) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). A rabbit polyclonal against human GLUT1 was purchased from Cell Marque (Rocklin, CA).
Spm498
Manufactured by Abcam
Sourced in United States
The SPM498 is a laboratory equipment product. It is designed for scientific applications. The core function of this product is to perform specific tasks and measurements in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Monoclonal mouse anti human cd68, by Agilent Technologies (1 mentions)
Cd45 2b11 pd7 26, by Agilent Technologies (1 mentions)
Cd11b m1 70, by BD (1 mentions)
Cd45 30 f11, by BD (1 mentions)
Mac3 m3 84, by BD (1 mentions)
Yap d24e4, by Cell Signaling Technology (1 mentions)
Envision flex kit, by Agilent Technologies (1 mentions)
Sp5 confocal microscope, by Leica (1 mentions)
3 protocols using spm498
1
Antibody Characterization for Vascular Research
2
GLUT-1 Immunohistochemistry in Liver
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Pretreatment of the sections was carried out for 30 minutes in a water bath at 97°C in low pH Flex antigen retrieval solution. Sections were incubated for 20 minutes with 1/600 anti-human GLUT-1 mouse monoclonal antibody (Abcam SPM498). Dako EnVision FLEX+ Kit was used and liver was the control tissue. Percentage membrane positive staining per HPF was estimated from an average of 10 HPFs assessed.
3
Immunofluorescence Microscopy of GLUT1
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Cells were grown in a four‐well chamber slide (Falcon) and fixed with 4% paraformaldehyde in phosphate‐buffered saline (PBS) for 20 min, permeabilized with 2% bovine serum albumin (BSA), and 0.1% saponin in PBS for 30 min at room temperature. Cells were incubated overnight at 4°C with the primary antibody against GLUT1 (Abcam, clone SPM498, 1:1,000) in 2% BSA and 0.1% saponin in PBS. Cells were washed three times in PBS and incubated with a secondary antibody. Confocal analysis was performed with a Leica SP5 confocal microscope.
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