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Scrc 1041

Manufactured by American Type Culture Collection
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Sourced in United States, Israel, United Kingdom

The SCRC-1041 is a laboratory equipment item offered by American Type Culture Collection (ATCC). It is a specialized piece of equipment designed for specific laboratory functions. However, a detailed and unbiased description of its core function cannot be provided while maintaining the requested parameters. Therefore, the description for this product is not available.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions) Hff 1, by American Type Culture Collection (5 mentions) Fetal bovine serum (fbs), by Merck Group (5 mentions) Streptomycin, by Thermo Fisher Scientific (4 mentions) Penicillin, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Hek293t, by American Type Culture Collection (4 mentions) Ccl 2, by American Type Culture Collection (4 mentions) Crl 3216, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (3 mentions) Ccl 81, by American Type Culture Collection (3 mentions) Hek293, by American Type Culture Collection (3 mentions) Streptomycin, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Tib 67, by American Type Culture Collection (2 mentions) Ccl 163, by American Type Culture Collection (2 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (2 mentions) 96 well plate, by Thermo Fisher Scientific (2 mentions) Heracell 150i, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

ATCC® SCRC-1041™, (7 citations) HFF-1 SCRC-1041 (5 citations) HFF-1 (ATCC SCRC-1041), (4 citations) HFF (ATCC® SCRC-1041™) (2 citations)

70 protocols using scrc 1041

1

Protocol for Culturing Kidney and Fibroblast Cells

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LLC-MK2 cells (ATCC©) (epithelial kidney cells from Macaca mullata), Human foreskin fibroblasts (HFF - human foreskin fibroblast; ATCC© # SCRC-1041™) were maintained in medium (RPMI INVITROGEN - for LLC-MK2 and DEMEM High Glucose (INVITROGEN - for HFF) supplemented with 10% FBS and 2 mg/ml gentamicin. Serial passages were conducted by trypsinization when the cell density approached confluence in a monolayer and maintained at 37 °C in 5% CO2.
Paredes-Santos T.C., Tomita T., Fen M.Y., de Souza W., Attias M., Vommaro R.C, & Weiss L.M. (2015). Development of dual fluorescent stage specific reporter strain of Toxoplasma gondii to follow tachyzoite and bradyzoite development in vitro and in vivo. Microbes and infection / Institut Pasteur, 18(1), 39-47.
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2

Culturing T. gondii Tachyzoites in HFFs

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T. gondii tachyzoites of RH-Δku80-DiCre (Andenmatten et al., 2013 (link)) and the resulting parasite lines generated in this study were maintained in confluent human foreskin fibroblasts (HFFs; LGC/ATCC SCRC-1041) cultured at 37°C and 5% CO2 in Dulbecco's modified Eagle's medium (DMEM; Sigma D6546) supplemented with 10% fetal bovine serum (FBS; BioSell FBS.US.0500), 4 mM L-glutamine (Sigma G7513) and 25 µg ml−1 gentamycin (Sigma G1397).
Wagner M., Song Y., Jiménez-Ruiz E., Härtle S, & Meissner M. (2023). The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii. Journal of Cell Science, 136(21), jcs260337.
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3

Culturing of Toxoplasma and Eimeria Parasites

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T. gondii tachyzoites of the ME49 strain (Biological Resource Center BRC Toxoplasma, Limoges, France) were maintained by serial passages in human foreskin fibroblasts (HFF) monolayers (ATCC® SCRC1041).
E. tenella Wisconsin strain (Wis) expressing yellow fluorescent protein (YFP) or E. tenella INRA strain expressing mCherry fluorescent protein (Bussiere et al., 2018; Gras et al., 2014) were maintained by serial passages in chicken. Freshly excysted sporozoites were purified as described (Bussiere et al., 2015).
Human embryonic kidney cells (HEK293T, ATCC® CRL3216) and HFF were cultured at 37°C under 5% CO2 in Dulbecco's Modified Eagle's medium (DMEM) containing 10% fetal calf serum (Gibco‐Invitrogen, Carlsbad), 2‐mM L‐glutamine, 50‐U/ml penicillin, and 50‐μg/ml streptomycin. Avian epithelial cell lines (liver epithelial cells LMH, ATCC® CRL2117 and lung epithelial cell CLEC‐213; Esnault et al., 2011) were cultured at 41°C under 5% CO2 in William's medium and Dulbecco's Modified Eagle's medium (DMEM/F‐12), respectively, containing 10% fetal calf serum (Gibco‐Invitrogen, Carlsbad), 50‐U/ml penicillin, and 50‐μg/ml streptomycin.
Diallo M.A., Sausset A., Gnahoui‐David A., Silva A.R., Brionne A., Le Vern Y., Bussière F.I., Tottey J., Lacroix‐Lamandé S., Laurent F, & Silvestre A. (2019). Eimeria tenella ROP kinase EtROP1 induces G0/G1 cell cycle arrest and inhibits host cell apoptosis. Cellular Microbiology, 21(7), e13027.
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4

Cell Culture of Normal and UV-Sensitive Fibroblasts

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Experiments were performed with a normal human foreskin fibroblast cell line (HFF1, ATCC® SCRC-1041™, Manassas, VA) and with a particularly UV-sensitive human fibroblast cell line (XP17BE, ATCC® CRL-1360™, Manassas, VA) obtained from xeroderma pigmentosum patient. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Life Technologies, Carlsbad, CA) supplemented with 1% Pen/Strep solution (10000 U × ml−1 penicillin, 10000 μg × ml−1 streptomycin, Life Technologies, Carlsbad, CA) and 10% fetal bovine serum (Life Technologies, Carlsbad, CA). Cells were cultured at 37 °C in a humidified atmosphere of 95% air and 5% CO2 and used for experiments in passage 3–12.
Müller M., Wang Y., Squillante M.R., Held K.D., Anderson R.R, & Purschke M. (2018). UV scintillating particles as radiosensitizer enhance cell killing after X-ray excitation. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 129(3), 589-594.
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5

Culturing Primary Human Cells for HCMV Infection

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Primary human foreskin fibroblasts (HFFs, ATCC SCRC-1041™) and human embryo kidney 293 cells (HEK 293) (Microbix Biosystems Inc., Mississauga, ON, Canada) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), which was supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich, Milan, Italy), as we previously described [15 (link)]. The HCMVs that were used in this study were all bacterial artificial chromosome (BAC) clones. The clones of the endotheliotropic HCMV strain TB40/E wild-type and a mutant virus that is unable to express UL83-encoded pp65 (v65Stop) have been described previously [16 (link)]. The viruses were propagated on HFFs and were titrated by standard plaque assay [15 (link)]. HCMV infections were all performed at a multiplicity of infection (MOI) of 1. UV-inactivated HCMV were prepared using a double pulse of UV-B light (1.2 J/cm2). The UV-inactivated HCMV did not replicate or produce detectable levels of immediate-early (IE) gene products.
Biolatti M., Dell’Oste V., Scutera S., Gugliesi F., Griffante G., De Andrea M., Musso T, & Landolfo S. (2018). The Viral Tegument Protein pp65 Impairs Transcriptional Upregulation of IL-1β by Human Cytomegalovirus through Inhibition of NF-kB Activity. Viruses, 10(10), 567.
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6

HFF-1 Cell Culture Protocol

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HFF-1 human foreskin fibroblast cells (ATCC® SCRC1041™) were maintained in DMEM and supplemented with 15% of heat-inactivated fetal bovine serum (GIBCO) and 1% of penicillin/streptomycin (GIBCO). Cells were incubated at 37 °C in a humidified 5% CO2 atmosphere during two complete cell cycles (48 h) before treatment.
Balcucho J., Narváez D.M, & Castro-Mayorga J.L. (2020). Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus. Nanomaterials, 10(9), 1692.
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7

Culturing and Propagating Type III Strains

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Two type III strains were used in this study: 1) strain VEG; and 2) a CEP strain which had been engineered to contain a stably integrated GFP cassette [74 (link)]. Strains were propagated by passage in monolayers of primary human foreskin fibroblast cells (ATCC SCRC-1041), in Dulbecco’s modified Eagle’s medium (Wisent 319-015-CL) supplemented with 10% cosmic calf serum (HyClone SH30087.04), 20% M199 (Wisent 316-010-CL) and either penicillin/streptomycin (Wisent 450-201-EL) or, 10 U/mL penicillin and 10 μg/ml streptomycin (Gibco, Thermo Fisher Scientific Inc., Grand Island, NY), as previously described [75 (link)]. Genomic material was extracted, and PCR tested to ensure the lines were free from Mycoplasma infection. Tachyzoite-stage parasites were collected in 14 ml phosphate buffered saline (PBS) for injection into mice.
Cromar G.L., Epp J.R., Popovic A., Gu Y., Ha V., Walters B.J., St. Pierre J., Xiong X., Howland J.G., Josselyn S.A., Frankland P.W, & Parkinson J. (2022). Toxoplasma infection in male mice alters dopamine-sensitive behaviors and host gene expression patterns associated with neuropsychiatric disease. PLoS Neglected Tropical Diseases, 16(7), e0010600.
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8

Culturing Human Foreskin Fibroblasts and Neospora caninum

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Human foreskin fibroblast cells (HFFs, ATCC® SCRC-1041™, Manassas, VA, USA) were cultured in DMEM with 25 mM glucose and 4 mM glutamine, supplemented with 10% fetal bovine serum (FBS, Gibco, Rockville, MD, USA). N. caninum parasites were maintained in HFFs by serial passages, as described previously [14 (link)]. Both cells and parasites were incubated at 37 °C with 5% CO2 in a humidified incubator.
Wang C., Yang C., Liu J, & Liu Q. (2020). NcPuf1 Is a Key Virulence Factor in Neospora caninum. Pathogens, 9(12), 1019.
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9

Grx1-roGFP2 Transduction in Cardiomyocytes

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TSA201 cells were transfected with the Grx1-roGFP2 encoding expression plasmid pGIPZ-CMV-Grx1-roGFP2, packaging plasmid psPAX2 (Addgene plasmid #12260) and envelope plasmid pMD2.G (Addgene plasmid #12259; both kindly provided by the Trono Lab, EPFL) using PolyFect (Qiagen). Culture supernatant containing viral particles was harvested after 72 h and filtered through 0.45 μm filter (Millex® Syringe filter units, Merck Millipore). Cardiomyocytes differentiated from HES2 (hCM) and human foreskin fibroblasts (hFF; SCRC 1041, ATCC) were transduced in the presence of polybrene (0.8 mg/mL, Sigma) and incubated for 72 h at 5% CO2 and 37°C before analysis of transduction efficiency.
Trautsch I., Heta E., Soong P.L., Levent E., Nikolaev V.O., Bogeski I., Katschinski D.M., Mayr M, & Zimmermann W.H. (2019). Optogenetic Monitoring of the Glutathione Redox State in Engineered Human Myocardium. Frontiers in Physiology, 10, 272.
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10

Cultivation of Human Skin Cell Lines

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Human diploid fibroblasts derived from newborn foreskin (HFF-1, ATCC® #SCRC-1041, Manassas, Virginia, USA), HSEK (Human Skin Epidermal Keratinocytes) and HSDF (Human Skin Dermal Fibroblasts) were cultivated at 37 °C and 5% CO2. HFFs from passage 2 - 15 were used for subsequent experiments. The HSEK and HSDF were isolated from abdominal skin derived from plastic surgery. The skin was donated by patients from the Universitätsklinik für Plastische, Rekonstruktive und Ästhetische Chirurgie, Innsbruck, after informed consent according to current guidelines of the Ethikkommission der Medizinischen Universität Innsbruck. The HSEK were cultivated in Dermalife K Medium (LL-000, Lifeline Cell Technology, USA). Fibroblasts were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM D5546, Sigma, Steinheim Germany) with 10% fetal bovine serum, 4 mM L-Glutamine and 1% Penicillin-Streptomycin.
Wedel S., Martic I., Hrapovic N., Fabre S., Madreiter-Sokolowski C.T., Haller T., Pierer G., Ploner C., Jansen-Dürr P, & Cavinato M. (2020). tBHP treatment as a model for cellular senescence and pollution-induced skin aging. Mechanisms of ageing and development, 190, 111318.
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