Upright light fluorescent microscope

Manufactured by Zeiss

The Upright Light/Fluorescent Microscope is a precision laboratory instrument designed for high-quality imaging and analysis. It features a stable upright configuration, capable of delivering bright-field and fluorescent illumination for a wide range of sample types. The microscope's core function is to provide users with a reliable and versatile platform for detailed examination and study of microscopic specimens.

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Lab products found in correlation

Axiocam camera, by Zeiss (3 mentions) Immpress excel amplified hrp polymer staining kit, by Vector Laboratories (1 mentions) M o m kit, by Vector Laboratories (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Sf100 4, by Thermo Fisher Scientific (1 mentions) Avidin d, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Fluorescent microscope (298 citations) Upright microscope (42 citations) Upright fluorescent microscope (11 citations) Upright light microscope (6 citations) Fluorescent light microscope (5 citations)

3 protocols using upright light fluorescent microscope

Histological Analysis of Liver Tissues

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Freshly collected liver tissues from MUP-uPA, MUP-uPA;gp130^Act, Reg3b^IEC and C57BL/6 mice were fixed in 10% neutral-buffered formalin or 4% paraformaldehyde, embedded in paraffin, sectioned and stained with hematoxylin and eosin (H&E) and Sirius Red. For frozen-block preparation, tissues were embedded in Tissue-Tek OCT compound and stained with Oil Red O and H&E. For Oil Red O and Sirius Red analysis, multiple images were examined for each section. Representative images were captured on an upright light/fluorescent microscope (Zeiss) equipped with an AxioCam camera.

Todoric J., Caro G.D., Reibe S., Henstridge D.C., Green C.R., Vrbanac A., Ceteci F., Conche C., McNulty R., Shalapour S., Taniguchi K., Meikle P.J., Watrous J.D., Moranchel R., Najhawan M., Jain M., Liu X., Kisseleva T., Diaz-Meco M.T., Moscat J., Knight R., Greten F.R., Lau L.F., Metallo C.M., Febbraio M.A, & Karin M. (2020). Fructose stimulated de novo lipogenesis is promoted by inflammation. Nature metabolism, 2(10), 1034-1045.

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Immunohistochemistry and TUNEL Staining of Formalin-Fixed Mouse Liver Samples

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Mouse liver samples were fixed with 10% neutral buffered formalin and paraffin embedded. 5μm thick sections were stained with hematoxylin and eosin (H&E) and processed for IHC. Vector Labs M.O.M kit (MP2400) was used for blocking endogenous mouse IgG when detecting mouse proteins using mouse primary antibodies. Vector Labs ImmPRESS^™ Excel Amplified HRP Polymer Staining Kit (MP7601) was used for IHCs that required signal amplification. Antibody details are provided in the Key Resource Table.
TUNEL staining was performed using an in-situ cell death detection kit (Roche# 12156792910). Images were captured on an upright light/fluorescent microscope (Zeiss) equipped with an AxioCam camera.

Dhar D., Antonucci L., Nakagawa H., Kim J.Y., Glitzner E., Caruso S., Shalapour S., Yang L., Valasek M.A., Lee S., Minnich K., Seki E., Tuckermann J., Sibilia M., Zucman-Rossi J, & Karin M. (2018). Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling. Cancer cell, 33(6), 1061-1077.e6.

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Liver Morphology and UGT1A Protein Analysis

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To analyze liver morphology, tissue samples were fixed in 10% buffered formalin phosphate (Fisher Chemicals, SF100-4), transferred to 70% ethanol, and processed at the UCSD Tissue Technology. Samples were embedded in paraffin, sliced into 5 μm sections, and stained with H&E (hematoxylin and eosin). For the staining of UGT1A proteins (Santa Cruz Technologies, sc-271268), paraffin liver sections were prepared in the UCSD Tissue Technology. Formalin-fixed, paraffin-embedded liver slides were deparaffinized and rehydrated, using xylene followed by alcohol and PBS washings. Antigen retrieval of tissue slides and the immunohistochemical staining with a primary antibody, secondary biotinylated antibody (BD Pharmingen, 550337), and Avidin D (Vector Laboratories, A-2004) were achieved as described previously (7) . Primary antibody was diluted 1:100, and secondary antibody was diluted 1:200. The images were captured on an upright light/fluorescent microscope (Zeiss) equipped with AxioCam camera.

Weber A.A., Yang X., Mennillo E., Wong S., Le S., Ashley Teo J.Y., Chang M., Benner C.W., Ding J., Jain M., Chen S., Karin M, & Tukey R.H. (2024). Triclosan administration to humanized UDP-glucuronosyltransferase 1 neonatal mice induces UGT1A1 through a dependence on PPARα and ATF4. The Journal of biological chemistry, 300(6).

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