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Tetrodotoxin

Manufactured by Biotium
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Tetrodotoxin is a highly potent neurotoxin that blocks voltage-gated sodium channels, inhibiting the transmission of action potentials in nerve and muscle cells. It is used as a research tool to study the function of sodium channels and their role in neural and muscular processes.

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Lab products found in correlation

Mifepristone, by Cayman Chemical (2 mentions) Quinine, by Thermo Fisher Scientific (1 mentions) Am251, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions)

Close spelling variants of this product

Tetrodotoxin (TTX)

3 protocols using tetrodotoxin

1

Pharmacological Modulation of Neuronal Signaling

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We purchased mifepristone from Cayman Chemical (Ann Arbor, MI), ethanol from Remet (La Mirada, CA) and tetrodotoxin from Biotium (Hayward, CA). Chemicals other than these were purchased from Sigma Aldrich (St. Louis, MO). CORT118335 was provided by Corcept Therapeutics (Menlo Park, CA). We prepared stock solutions of AP-5, CGP55845A and tetrodotoxin in distilled water, while DNQX, mifepristone and CORT118335 were dissolved in 100% DMSO. All drugs including ethanol were applied to the bath solution to achieve the final desired concentrations. The final DMSO concentration did not exceed 0.15% in any of the recordings given DMSO effects at higher concentrations on neuronal viability or interference with synaptic transmission (Nakahiro et al., 1992 (link); Tsvyetlynska et al., 2005 (link); Zhang et al., 2017 (link)).
Khom S., Rodriguez L., Gandhi P., Kirson D., Bajo M., Oleata C.S., Vendruscolo L.F., Mason B.J, & Roberto M. (2022). Alcohol dependence and withdrawal increase sensitivity of central amygdalar GABAergic synapses to the glucocorticoid receptor antagonist mifepristone in male rats. Neurobiology of disease, 164, 105610.
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2

Acute Slice Recordings and Behavioral Experiments

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DO34—3-(Phenylmethyl)−4-[[4-[4-(trifluoromethoxy) phenyl]−1H-1,2,3-triazol-1-yl] carbonyl]−1-piperazinecarboxylic acid 1,1-dimethylethyl ester (Glixx Laboratories Inc, CAS #:1848233-58-8), AM251 (Tocris), alcohol (Sigma, St. Louis, MO), and tetrodotoxin (TTX, Biotium, Fremont, CA) were diluted to their final concentration in aCSF for acute slice recordings. For behavioral experiments, alcohol, sucrose (Sigma, St. Louis, MO), and quinine (Alfa Aesar, Tewksbury, MA) were diluted in water.
Augustin S.M., Gracias A.L., Luo G., Anumola R.C, & Lovinger D.M. (2023). Striatonigral direct pathway 2-arachidonoylglycerol contributes to ethanol effects on synaptic transmission and behavior. Neuropsychopharmacology, 48(13), 1941-1951.
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3

Pharmacological Modulation of Neuronal Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
We purchased mifepristone from Cayman Chemical (Ann Arbor, MI), ethanol from Remet (La Mirada, CA) and tetrodotoxin from Biotium (Hayward, CA). Chemicals other than these were purchased from Sigma Aldrich (St. Louis, MO). CORT118335 was provided by Corcept Therapeutics (Menlo Park, CA). We prepared stock solutions of AP-5, CGP55845A and tetrodotoxin in distilled water, while DNQX, mifepristone and CORT118335 were dissolved in 100% DMSO. All drugs including ethanol were applied to the bath solution to achieve the final desired concentrations. The final DMSO concentration did not exceed 0.15% in any of the recordings given DMSO effects at higher concentrations on neuronal viability or interference with synaptic transmission (Nakahiro et al., 1992 (link); Tsvyetlynska et al., 2005 (link); Zhang et al., 2017 (link)).
Khom S., Rodriguez L., Gandhi P., Kirson D., Bajo M., Oleata C.S., Vendruscolo L.F., Mason B.J, & Roberto M. (2022). Alcohol dependence and withdrawal increase sensitivity of central amygdalar GABAergic synapses to the glucocorticoid receptor antagonist mifepristone in male rats. Neurobiology of disease, 164, 105610.
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