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Anti myeloperoxidase mpo

Manufactured by Agilent Technologies

Anti-myeloperoxidase (MPO) is a laboratory equipment product that detects the presence of myeloperoxidase, an enzyme found in certain white blood cells. It is used to measure MPO levels in clinical samples.

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3 protocols using anti myeloperoxidase mpo

1

Immunohistochemical Evaluation of Esophageal Protein Expression

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The protein expression of resected esophageal specimens was evaluated by immunohistochemistry. After pretreatment, each section was treated with the following primary antibodies: anti-pancytokeratin, anti-Ki67, anti-myeloperoxidase (MPO) (Dako), anti-inducible nitric oxide synthase (iNOS) (Abcam), and anti-CD3 (Abcam). All sections were treated with a secondary antibody (EnVision™/HRP, Dako), and hematoxylin was used for counterstaining. The clone, code, dilution, and antigen retrievals of immunohistochemistry are summarized in Supplemental Table 1.
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2

Immunostaining of Thrombosis Biomarkers

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Freshly recovered thrombi were fixed in 3.7% paraformaldehyde for 48 h before being embedded longitudinally in paraffin and sectioned at 5 µm. Immunostainings were performed as described previously27 (link) using the following primary antibodies: anti‐fibrinogen (20 µg/ml; Dako, ref A0080), anti‐Von Willebrand factor (10 µg/ml; Abcam, ref ab11713), anti‐CD42b (2 µg/ml; Beckman Coulter, ref IM0409), anti‐platelet factor 4 (10 µg/ml; Peprotech, ref 500‐P05), anti‐myeloperoxidase (MPO, 10 µg/ml; Dako, ref A0398), anti‐histone H4 citrulline 3 (H4Cit, 1/200; Millipore, ref 07‐596), anti‐angiotensin‐converting enzyme 2 (ACE‐2, 3 µg/ml, R&D Systems, ref AF933), and anti‐SARS‐CoV‐2 Spike Protein (5 µg/ml, P06DHuRb and T01KHuRb from Invitrogen, 10 µg/ml 40150‐R007 from Sino Biological). Red blood cells were identified by their autofluorescence (λex 440/9/λem > 570 nm).
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3

Immunofluorescence Staining and Histopathology

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Endothelial function studies were performed as reported by us.11 (link) Immunofluorescence staining for kidney IgG and C3 was performed on frozen sections as described.11 (link) Skin and kidney frozen sections were stained with rabbit polyclonal anti-myeloperoxidase (MPO) (Dako) and rat monoclonal anti-Ly-6G (eBioscience). For kidney histopathology, formalin-fixed sections were scored in a blinded manner as described.11 (link) Formalin-fixed kidney and skin sections were also stained with anti-Mx1 (Proteintech).
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