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Tskgel gmpwxl

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The TSKgel GMPWXL is a size exclusion chromatography column designed for the analysis of high molecular weight proteins and macromolecules. The column features a hydrophilic gel-type packing material that allows for the separation of large biomolecules with minimal adsorption and denaturing effects.

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Lab products found in correlation

Potassium persulfate, by Merck Group (3 mentions) Prominence gpc series, by Shimadzu (3 mentions) Tskgel g3000pwxl, by Tosoh (3 mentions) Rid 10a differential, by Shimadzu (2 mentions) Tskgel 2500 pwxl, by Tosoh (2 mentions) Rid 20, by Shimadzu (2 mentions) Tskgel g2500pwxl, by Tosoh (2 mentions) Tskgel guard column pwxl, by Tosoh (2 mentions) Acrylamide, by Fujifilm (1 mentions) Methacrylic acid, by Fujifilm (1 mentions) Carbopac pa20 column, by Thermo Fisher Scientific (1 mentions) Dx500, by Thermo Fisher Scientific (1 mentions) Astra 4, by Wyatt Technology (1 mentions) Dawn dsp f, by Wyatt Technology (1 mentions) Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Redifrac fraction collector, by GE Healthcare (1 mentions) Lc 20, by Shimadzu (1 mentions) Polycal, by Malvern Panalytical (1 mentions) Pullulan standard, by Merck Group (1 mentions) Ecosec software, by Tosoh (1 mentions)

20 protocols using tskgel gmpwxl

1

Polymerization of Acrylamide Monomer

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Example 5

This was carried out using the polymerization apparatus shown in FIG. 1. An aqueous solution containing 10 weight % acrylamide (Wako Pure Chemical Industries, Ltd.) and 0.1 weight % potassium persulfate (Sigma-Aldrich Co. LLC.) was mixed with hot water that had been preheated to 200° C. The recovered reaction solution was evaporated to dryness; the polymerization conversion as measured by gravimetry was 54.1%. The residue was redissolved in water and was analyzed by size exclusion chromatography (instrument: Prominence GPC series, Shimadzu Corporation; detector RID-10A differential refractometer, Shimadzu Corporation; column: TSKgel GMPWXL, Tosoh Corporation): the obtained molecular weights were a weight-average molecular weight (Mw) of 39,600 and a number-average molecular weight (Mn) of 11,000; the molecular weight distribution (Mw/Mn) was 3.60; and a polymer having a relatively uniform length was thus obtained.

US10442873B2. Radical polymerization method and polymerization reaction apparatus (2019-10-15). Japan Agency for Marine-Earth Science and Technology [JP]. Inventors: Shigeru Deguchi [JP], Keigo Kinoshita [JP].
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2

Polysaccharide Characterization by HPSEC

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The homogeneity and molecular weight distribution of the purified polysaccharideenriched extracts were estimated by high performance size exclusion chromatography (HPSEC) using two different columns (300 X 7.8 mm i.d., Tosoh Bioscience LLC, King of Prussia, PA) in sequence TSKgel GMPWXL (dextran MW<50000 kDa) and TSKgel G3000PWXL (dextran MW<60 kDa) as described previously. 22 The system was calibrated with standard dextrans of 252, 110, 70, 40, and 6 kDa, and glucose, using a regression curve.
Bermúdez-Oria A., Rodríguez-Gutiérrez G., Rubio-Senent F., Sánchez-Carbayo M, & Fernández-Bolaños J. (2020). Antiproliferative Activity of Olive Extract Rich in Polyphenols and Modified Pectin on Bladder Cancer Cells. Journal of medicinal food, 23(7).
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3

Synthesis of Methacrylic Acid Polymer

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Example 6

This was carried out using the polymerization apparatus shown in FIG. 1. An aqueous solution containing 10 weight % methacrylic acid (Wako Pure Chemical Industries, Ltd.) and 0.1 weight % potassium persulfate (Sigma-Aldrich Co. LLC.) was mixed with hot water that had been preheated to 200° C. The recovered reaction solution was evaporated to dryness; the polymerization conversion as measured by gravimetry was 10.4%. The residue was redissolved in water and was analyzed by size exclusion chromatography (instrument: Prominence GPC series, Shimadzu Corporation; detector: RID-10A differential refractometer, Shimadzu Corporation; column: TSKgel GMPWXL, Tosoh Corporation): the obtained molecular weights were a weight-average molecular weight (Mw) of 22,500 and a number-average molecular weight (Mn) of 9,040; the molecular weight distribution (Mw/Mn) was 2.49; and a polymer having a relatively uniform length was thus obtained.

US10442873B2. Radical polymerization method and polymerization reaction apparatus (2019-10-15). Japan Agency for Marine-Earth Science and Technology [JP]. Inventors: Shigeru Deguchi [JP], Keigo Kinoshita [JP].
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4

Monosaccharide Composition and Molecular Weight Determination

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The POS and MCP monosaccharide composition was analyzed, following methanolysis (Manderson, et al., 2005) , by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), using a DX-500 (Dionex, Sunnyvale, CA, USA) system and a CarboPac PA-20 column operated at 0.5 ml/min, as described previously (Hotchkiss, Nunez, Strahan, Chau, White, Marais, et al., 2015) . The HPAEC-PAD mobile phase consisted of 14 mM NaOH for 13 min, followed by a 0-120 mM CH3COONa gradient in 100 mM NaOH for 17 min and it was returned to 14 mM NaOH for 40 min prior to the next injection. Molecular weight (MW) was determined by high pressure size exclusion chromatography (HPSEC), with three TSKgel GMPWXL (Tosoh Bioscience, Tokyo, Japan) columns and four detectors (HELEOS II multi-angle laser light scattering, refractive index, 255-V2 differential pressure viscometer; Wyatt Technology, Santa Barbara, CA, USA) and a UV-1260 Infinity spectrophotometer (Agilient Technologies, Santa Clara, CA, USA), as reported previously (Qi, Chau, Fishman, Wickham, & Hotchkiss, 2014) . MW values reported are weight average molar mass values. The degree of methyl esterification was determined as described previously (Fishman, Chau, Cooke, & Hotchkiss, 2008) .
Di R., Vakkalanka M.S., Onumpai C., Chau H.K., White A., Rastall R.A., Yam K, & Hotchkiss AT J.r. (2017). Pectic oligosaccharide structure-function relationships: Prebiotics, inhibitors of Escherichia coli O157:H7 adhesion and reduction of Shiga toxin cytotoxicity in HT29 cells. Food chemistry, 227.
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5

Analyzing Molecular Weight Distribution

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The homogeneity and molecular weight distribution of the purified extracts were estimated by high performance size-exclusion chromatography (HPSEC) using two different columns (300 × 7.8 mm i.d., Tosoh Bioscience LLC, King of Prussia, PA, USA) in sequence TSK gel GMPWXL (dextran Mw < 50,000 kDa) and TSKgel G3000PWXL (dextran Mw < 60 kDa), as described previously [25 (link)]. The system was calibrated with standard dextrans of 500, 110, 70, 40, and 6 kDa.
Bermúdez-Oria A., Rodríguez-Juan E., Rodríguez-Gutiérrez G., Fernández-Prior Á, & Fernández-Bolaños J. (2021). Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin–Polyphenols and Their Antioxidant and Antiproliferative Activities. Antioxidants, 10(12), 1858.
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6

Starch Polysaccharide Molecular Weight Analysis

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A high-performance size exclusion chromatography (HPSEC) system with a multiangle laser light scattering (MALLS) detector and a differential refractive index detector (RI) were used to determine the molecular weight and radii of gyration of the starch polysaccharide molecules. The high-performance size exclusion chromatography (HPSEC) system consisted of a pump (Ultimate 3000, Dionex, Palo Alto, CA, USA), an injection valve (model 7021, Rheodyne, Palo Alto, CA, USA), a guard column (TSK PWH, Tosoh Corporation, Tokyo, Japan) and two connected size exclusion columns: TSKgel GMPWXL (300 mm × 7.8 mm, Tosoh Corporation, Tokyo, Japan) and TSKgel 2500 PWXL (300 mm × 7.8 mm, Tosoh Corporation, Tokyo, Japan). A multiangle laser light scattering (MALLS) detector (Dawn-DSP-F, Wyatt Technology, Santa Barbara, CA, USA) and a differential refractive index detector (model SE71, Shodex, Tokyo, Japan) were connected to the columns.
The flow rate of the mobile phase and the sample injection volume were 0.4 mL · min−1 and 500 μL, respectively. Calculation of the weight-average molecular weight (Mw) and radius of gyration (Rg) were performed using Astra 4.70 software (Wyatt Technology, Santa Barbara, CA, USA).
Khachatryan K., Khachatryan L., Krzan M., Krystyjan M., Krzemińska-Fiedorowicz L., Lenart-Boroń A., Koronowicz A., Drozdowska M, & Khachatryan G. (2021). Formation and Investigation of Physicochemical, Biological and Bacteriostatic Properties of Nanocomposite Foils Containing Silver Nanoparticles and Graphene Oxide in Hyaluronic Acid Matrix. Materials, 14(12), 3377.
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7

Molecular Weight Analysis of Fraction B

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Analliquot of Fraction B was freeze-dried and redissolvedin 0.05 Mtris-hydrochloric acid. The method used for HPSEC analysis was that described by Jiménez et al. (Jiménez, Rodríguez, Fernández-Caro, Guillén, Fernández-Bolaños, & Heredia, 2001b ) with slight modifications. The MW was measured in Jasco equipment (LC-Net II ADC, Kyoto, Japan) with a refractive index detector (Jasco RI-1530) and injection valve (Rheodyne, loop 20 µL, Cotati, CA). Two different columns in sequence were used: TSKgelGMPWxl and TSKgel G3000PWxl (300 x 7.8 mmi.d., Tosoh Bioscience LLC, King of Prussia, PA) after calibration with 500, 110, 40, 6 kDa and maltose (Fluka, Buchs, Switzerland) . Blue dextran was used to test the void volume (V0) of the column. The elution buffer was 0.05 Mtris-hydrochloric acid at a flow rate 0.4 mL/min.Fractions of 250 L were collected using a Redifrac® fraction collector (Pharmacia Biotech, Uppsala, Sweden). Fractions were assayed for neutral sugars by the anthronemethod.
Mrabet A., Rodríguez Gutiérrez G., Rubio-Senent F., Hamza H., Rodríguez-Arcos R., Guillén-Bejarano R., Sindic M, & Jiménez-Araujo A. (2017). Enzymatic conversion of date fruit fiber concentrates into a new product enriched in antioxidant soluble fiber. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 75.
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8

Molecular Weight Determination of AMP by HPLC-RID

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The molecular weight of AMP was determined by HPLC-RID (LC-20, Shimadzu, Japan; RID-20, Shimadzu, Japan) equipped with an aqueous gel column (TSKgel GMPWXL, 7.5 mm × 300 mm, TOSOH, Tokyo, Japan). The parameter settings were as follows: the injection volume was 20 µL; the mobile phase was 0.1 mol/L NaNO3 + 0.06% NaN3 solution; the flow rate was 0.6 mL/min; the column temperature was 35 °C.
Liu B, & Tan Z. (2022). Separation and Purification of Astragalus membranaceus Polysaccharides by Deep Eutectic Solvents-Based Aqueous Two-Phase System. Molecules, 27(16), 5288.
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9

Molecular Weight Analysis of SDF

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The molecular weight of SDF was analyzed by gel permeation chromatography (GPC). Samples (10 mg) of SDF from untreated and SE-treated adlay bran were dissolved in 1 mL ultrapure water. Then, the mixture was filtered by 0.45 μm membranes. The chromatographic conditions were as follows: gel chromatography column was TSKgel GMPWXL (TOSOH CORP., Japan), mobile phase was 0.1 mol/L NaCO3 at a flow rate of 0.6 mL/min, and injection volume was 100 μL.
Tang X., Wang Z., Zheng J., Kan J., Chen G, & Du M. (2023). Physicochemical, structure properties and in vitro hypoglycemic activity of soluble dietary fiber from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) bran treated by steam explosion. Frontiers in Nutrition, 10, 1124012.
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10

Molecular Weight and Chain Length Determination of Starch Using SEC

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The distributions of molecular weight and chain length of the AS were determined by size-exclusion chromatography (SEC), with which the molecules were separated based on size or hydrodynamic volume [22 (link)]. A solution of starch (20 mg) in 4 mL of DMSO (90% in distilled water, v/v) was heated at 100 °C for 15 min and then cooled to 25 °C under continuous stirring for 24 h. Subsequently, the mixture was diluted with 20 mL of 95% (v/v) ethanol and centrifuged at 3500× g for 10 min. The supernatant was discarded, and the pellet was reconstituted in 4 mL of deionized water, and the resulting suspension was stirred in a water bath at 100 °C for 15 min. The mixture was cooled to 25 °C and filtered through a 5-μm sieve, and then the filtrate was passed through a gel-permeation chromatography column equipped with refractive index detector (RID-20, Shimadzu, Kyoto, Japan). The mobile phase was an aqueous solution of 0.06% NaN3 (ultrasonicated before injection), the flow rate was 0.2 mL/min and the SEC column (TSK gel GMPWXL, TOSOH, Tokyo, Japan) temperature was 60 °C. The ASTRA 6.1 software package (Wyatt Technology Inc., Goleta, CA, USA) was used to determine the molecular weight, from which the polydispersity index (D) was calculated based on the following equation:
where Mw is the weight average molecular weight, and Mn is the number average molecular weight.
He M., Ding T., Wu Y, & Ouyang J. (2022). Effects of Endogenous Non-Starch Nutrients in Acorn (Quercus wutaishanica Blume) Kernels on the Physicochemical Properties and In Vitro Digestibility of Starch. Foods, 11(6), 825.
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