C9100 13 emccd camera

Fixed samples were imaged using an inverted Leica SP5 confocal microscope. For representative images, a 60 × /1.40 N.A oil immersion objective was used. Live cell imaging was performed on a UltraView Vox spinning disc confocal microscope (Perkin Elmer Nikon TiE; Yokogawa CSU-X1 spinning disc scan head) with 60 × /1.40 N.A oil objective and equipped with a Hamamatsu C9100-13 EMCCD camera, or a 3I spinning disc confocal microscope (Zeiss AxioObserver Z1; Yokogawa CSU-W1 spinning disc scan head) with 63 × /1.40 N.A objective and equipped with a photometrics prime 95B scientific CMOS camera. Both spinning disc microscopes are equipped with a temperature-controlled environment chamber set at 26C for the experiments. Airyscan pictures were taken on fixed and stained neuroblasts using an inverted LSM880 Multiphoton or on live samples using a LSM900 point scanning laser confocal with Airyscan 2 on an inverted Axio Observer Z1 microscope stand.