Sfrp2

Manufactured by R&D Systems

Sourced in United States, Mongolia

SFRP2 is a recombinant human protein that belongs to the secreted frizzled-related protein (SFRP) family. SFRPs are known to function as modulators of the Wnt signaling pathway.

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Lab products found in correlation

Sb202190, by Merck Group (1 mentions) Kn 93, by Merck Group (1 mentions) Ksom medium, by Merck Group (1 mentions) Spinning disc ix81 microscope, by Olympus (1 mentions)

4 protocols using sfrp2

Wnt Signaling Regulates Embryo Attachment

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Embryos were treated in KSOM medium (Sigma-Aldrich) with 10% Wnt5a CM or Wnt7a CM or control CM for 48 h before immunofluorescence. After treatment embryos were washed and cultured in KSOM medium (Sigma-Aldrich) for one additional hour prior to the FN-binding assay. Where indicated, the embryos were pre-treated with 5 μm KN93, 1 μm Wortmanmin, or 5 μm SB202190 (all from Sigma-Aldrich) for 30 min before adding Wnt CM.
For in vivo experiments, hatched blastocysts either alone, or with 500ng of sFRP2 (R&D Systems, Minneapolis, MN), or with sFRP2 plus 10% Wnt7a CM, were transferred into the uterine horn of pseudopregnant females on day 4. The embryos were flushed out from the uterine horn 5 min after injection and then cultured in KSOM medium (Sigma-Aldrich) for one additional hour before the FN-binding assay.

Lou Y., Pinel L, & Dufort D. (2023). Uterine WNTS modulates fibronectin binding activity required for blastocyst attachment through the WNT/CA2+ signaling pathway in mice. Reproductive Biology and Endocrinology : RB&E, 21, 85.

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Activation Assays for Rho GTPases

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For RhoA, Cdc42 and Rac1 activation assays, the glioblastoma tissues were grinded in liquid nitrogen. Equal weights of total tissue debris were dissolved in ice-cold PBS buffer. Glioblastoma cells were seeded into 6-well plates and transfected with Daam1-siRNA or treated with sFRP2 (R&D Systems, Minneapolis, MN, USA). The experiments were then performed according to the manufacturer's protocol (Cytoskeleton Inc., Denver, CO, USA). The activation of RhoA, Cdc42 and Rac1 was normalized to the NC control group.

Liu G., Yan T., Li X., Sun J., Zhang B., Wang H, & Zhu Y. (2017). Daam1 activates RhoA to regulate Wnt5a-induced glioblastoma cell invasion. Oncology Reports, 39(2), 465-472.

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Wnt Inhibition Attenuates Anal Sphincter Fibrosis

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To test the hypothesis that Wnt inhibition will attenuate collagen deposition and improve EAS muscle function, a group of rabbits were subjected to EAS myotomy (experimental; n = 12), immediately after which they received a local injection at the myotomy site that consisted of either a Wnt antagonist, secreted frizzled receptor protein, sFRP-2 (R&D systems, MN), 2 µg in 200 µl saline (n = 6) or an equal volume of 0.9% saline (placebo), (n = 6). Injection was blinded to minimize operator bias and repeated every day for 7 days at the myotomy site. Animals were sacrificed at the end of the study (15 weeks) and the anal canal was harvested and fixed in formalin to perform the following tissue analysis: (1) Histology & histochemical evaluation to determine fibrosis/collagen content³⁵. (2) Immuno-histochemistry/ immuno-fluorescence to localize proteins involved in fibrosis/connective tissue formation. (3) Western blots to quantify relevant proteins. (4) Quantitative PCR to determine changes in gene expression.

Rajasekaran M.R., Kanoo S., Fu J., Bhargava V, & Mittal R.K. (2017). Wnt-β Catenin Signaling Pathway: A Major Player in the Injury Induced Fibrosis and Dysfunction of the External Anal Sphincter. Scientific Reports, 7, 963.

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Intracellular Ca2+ Dynamics in Hippocampal Neurons

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Intracellular Ca²⁺ measurements were carried out in DIV10 hippocampal neurons loaded with the ratiometric probe Fura-2 AM at 4.5 µM for 30 min. Before the assay, neurons were treated for 1 h with control or Wnt5a medium or with Wnt5a plus 1 µM HRI-i (JANSEN), 10 µM 7NI (SIGMA) or 250 nM sFRP2 (R&D systems), with a pretreatment of 30 min for all of these inhibitors. The experiments were performed with an isotonic solution containing (in mM): 2.5 CaCl₂, 132 NaCl, 4 KCl, 10 NaHCO₃, 6 glucose, and 10 HEPES (305 mOsm/liter, pH7.4). Cytosolic Ca²⁺ levels were continuously recorded (each 5 s) for 1 min before (basal levels) and 6 min after application of 100 µM NMDA + 100 µM glycine. Variations in cytosolic Ca²⁺ are presented as the normalized ratio of the emitted fluorescence at 510 nm after excitation at 340 and 380 nm relative to the ratio measured prior to stimulation (first minute before application of the stimuli), and the area under the curve (AUC) after the addition of NMDA and glycine was integrated. Live Ca²⁺ imaging was performed with an Olympus spinning disc IX 81 microscope.

Ramos-Fernández E., Arrázola M.S., Oliva C.A., Arredondo S.B., Varela-Nallar L, & Inestrosa N.C. (2021). Wnt5a promotes hippocampal postsynaptic development and GluN2B-induced expression via the eIF2α HRI kinase. Scientific Reports, 11, 7395.

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