Lu n4 laser launch

Manufactured by Nikon

The LU-N4 laser launch is a compact and versatile device designed for launching laser beams. It provides a stable and controlled output for various laser-based applications. The LU-N4 features precise beam alignment and a robust construction, making it suitable for use in research, industrial, and laboratory settings.

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Lab products found in correlation

Eclipse ti e, by Nikon (2 mentions) Spectra x led light engine, by Lumencor (1 mentions) Orca 4.0 v2 scmos camera, by Hamamatsu Photonics (1 mentions) Ti2 widefield microscope, by Nikon (1 mentions) 1.4na oil immersion objective lens, by Nikon (1 mentions)

3 protocols using lu n4 laser launch

Spinning disk confocal microscopy

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All fluorescent images were obtained using a Nikon Eclipse Ti-E inverted microscope stand that has a Yokogawa, two-camera, CSU-W1 spinning disk system with a Nikon LU-N4 laser launch. All images were obtained on ×100 PlanAPO objective lens.

Delgado J.M., Shepard L.W., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover. The EMBO Journal, 43(1), 32-60.

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Multimodal Live Cell Imaging Setup

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The fixed smFISH-IF samples were imaged on an automated inverted Nikon Ti-2 wide-field microscope equipped with a 60× 1.4NA oil immersion objective lens (Nikon), Spectra X LED light engine (Lumencor), and Orca 4.0 v2 scMOS camera (Hamamatsu).
The live cell imaging was conducted on a customized wide-field microscope built around a Nikon Ti-E stand. The microscope was equipped with three separate EMCCD cameras (Andor iXon Ultra 897) with ultra-flat 2 mm thick imaging splitting dichroic mirrors (T565LPXR-UF2, T640LPXR-UF2). Each of the cameras was equipped with band pass emission filter (ET525/50m, ET595/50m, and ET655lp). The light source of the microscope is an LU-n4 laser launch (Nikon) with four wavelengths: 405, 488, 561, and 640 nm. The excitation was done through H-TIRF with 100× 1.49NA oil immersion TIRF objective (Nikon). The excitation laser and fluorescence emission were separated by a quad band dichroic mirror (Chroma, ET-405/488/561/640 nm). The microscope was also equipped with an automated linear encoded XY-stage with Piezo-Z (Applied Scientific Instrumentation).

Latallo M.J., Wang S., Dong D., Nelson B., Livingston N.M., Wu R., Zhao N., Stasevich T.J., Bassik M.C., Sun S, & Wu B. (2023). Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD. Nature Communications, 14, 5581.

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Spinning Disk Confocal Imaging Protocol

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Delgado J.M., Wallace Shepard L., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy. bioRxiv.

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