Primescript rt master mix real time rt pcr kit

Manufactured by Takara Bio

The PrimeScript RT Master Mix Real-time RT-PCR Kit is a laboratory product that enables reverse transcription and real-time PCR amplification in a single reaction. It contains pre-mixed reagents necessary for these processes.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Sybr green supermix kit, by Bio-Rad (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Kapa sybr fast qpcr kit, by Roche (1 mentions)

Close spelling variants of this product

PrimeScript™ real-time PCR (RT-PCR) Kit PrimeScript Real-Time Master Mix PrimeScript™ real-time PCR kit PrimeScript RT Master Mix PrimeScript RT-PCR mix PrimeScript RT-PCR kit PrimeScript RT Mix PrimeScript Master Mix RT Master Mix PrimeScript RT kit

2 protocols using primescript rt master mix real time rt pcr kit

Time-Series Analysis of Gene Expression

Cited 1 time

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Total RNA was extracted from cells using TRIzol (Thermo Fisher). cDNA was synthesized using a PrimeScript RT Master Mix Real-time RT-PCR Kit (Takara) and analyzed by qRT-PCR with a SYBR Green Supermix kit (Bio-rad). For the time course qPCR, cells were synchronized using a 50% serum shock for two hours, followed by harvesting at six time points over 24 hours. Gene primers for qPCR are listed in Table S3, and rhythmicity was analyzed using JTK_Cycle.⁶⁴ (link)

Zhang H., Chen C., Zhang E.E, & Huang X. (2024). TDP-43 deficiency in suprachiasmatic nucleus perturbs rhythmicity of neuroactivity in prefrontal cortex. iScience, 27(4), 109522.

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Gene Expression Analysis via qRT-PCR

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RNA was extracted from cells and mouse hearts with Trizol reagent according to the manufacturer’s instructions (#15596, Life Technologies). cDNA was generated from RNA using a PrimeScript RT Master Mix Real-time RT-PCR Kit (#RR036A, Takara). Gene expression was analyzed via quantitative real-time PCR (qRT-PCR) with a KAPA SYBR FAST qPCR Kit (#KP-KK4601, Kapa Biosystems). The reactions were first incubated at 95°C for 10 min, followed by 40 cycles at 95°C for 10 s, 60°C for 30 s. Gene-specific primer sequences are as follows: hTBP, 5'-GAACCACGGCACTGATTTTC-3' and 5'-CCCCACCATGTTCTGAATCT-3', hPCBP1, 5'-GGACAACACACCATTTCTCCGC-3' and 5'-AGCCTTTCACCTCTGGAGAGCT-3'; hPCBP2, 5'-ATCTGCTGCCAGCATTAGCCTG-3' and 5'-GGTGGTGAACAGCAGAAAGGGA-3'; mTbp, 5'-CAAACCCAGAATTGTTCTCCTT-3' and 5'-ATGTGGTCTTCCTGAATCCCT-3'; mPcbp1, 5'-GGACAACACACCATTTCTCCGC-3' and 5'-AGCCTTTCACCTCTGGAGAGCT-3'; mBmal1, 5'-CTCGACACGCAATAGATGGGA-3' and 5'-CTTCCTTGGTCCACGGGTT-3'; mPer2, 5'-GAAAGCTGTCACCACCATAGAA-3' and 5'-AACTCGCACTTCCTTTTCAGG-3'; mCry1, 5'-GGTTGCCTGTTTCCTGACTCGT-3' and 5'-GACAGCCACATCCAACTTCCAG-3'.

Wu Y., Zhao H., Zhang E.E, & Liu N. (2021). Identification of PCBP1 as a Novel Modulator of Mammalian Circadian Clock. Frontiers in Genetics, 12, 656571.

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