Equal amounts of protein samples were separated via sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After blocking with 5% blocking reagent for 1 hour, the membranes were incubated with primary antibodies against OS‐9 (1:1000, ab19853; Abcam), HIF‐1α (1:1000, ab179483; Abcam), and beta actin (1:5000, ab6276; Abcam) at 4°C overnight. After washing with Tris‐buffered saline containing 0.1% Tween 20, the membranes were incubated for 2 hours with horseradish peroxidase‐coupled anti‐rabbit immunoglobulin G (1:2500, ab6721; Abcam) at room temperature. Densitometry analysis of protein bands was performed using the Millipore ECL Western Blot Analysis Substrate (Merck Millipore). All bands were analyzed using the ImageJ software (Version 2.1.0; National Institutes of Health).
Millipore ecl western blot analysis substrate
Manufactured by Merck Group
Sourced in Germany
Millipore ECL Western Blot Analysis Substrate is a chemiluminescent detection reagent used in Western blot analysis. It is designed to enable the visualization of target proteins that have been separated by gel electrophoresis and transferred to a membrane.
Automatically generated - may contain errors
2 protocols using millipore ecl western blot analysis substrate
1
Western Blot Analysis of OS-9, HIF-1α, and Beta Actin
2
Western Blot Analysis of Liver Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was performed to examine the expression of the specific target proteins. Total protein fractions from mouse liver were extracted, separated by gel electrophoresis, and transferred onto PVDF membranes (Millipore, MA, USA). Primary antibodies were incubated at 4 °C overnight and listed in Supplementary Table 1. Protein band densitometry was performed using Millipore ECL Western Blot Analysis Substrate (Merck Millipore, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!