Hd15 scanning electron microscope

The effect of the PEF pretreatment and the cascade process on the morphological characteristics of cherry pomace cell tissues was assessed using Scanning Electron Microscopy (SEM), as described by Pirozzi et al. [42 (link)]. The dried cherry pomace was mounted on an aluminum stub and coated by a 10 nm thick gold-palladium alloy sputter coater before being analyzed in a high-resolution ZEISS HD15 Scanning Electron Microscope (Zeiss, Oberkochen, Germany) at 1000× magnification.

The morphology of celluloses was observed with an optical microscope (Nikon Eclipse TE 2000S, Nikon instruments Europe B.V., Amsterdam, The Netherlands) with a 10× objective, coupled to a DS Camera Control Unit (DS-5M-L1, Nikon Instruments Europe B.V, Amsterdam, The Netherlands) for image acquisition and analysis. For scanning electron microscope (SEM) observations, the samples were mounted on an aluminum stub and coated by a 10 nm thick gold-palladium alloy sputter coater before observation in a high-resolution ZEISS HD15 Scanning Electron Microscope (Zeiss, Oberkochen, Germany) at 20,000× magnification.

The morphological features of coatings were analyzed also using scanning electron microscopy (SEM). Slices, 2 mm thick cut out of coated tomatoes, were fixed by immersion in a 2% (v/v) glutaraldehyde phosphate buffer solution. The buffer was removed, and the slices were osmotically dehydrated with ethanol solutions of increasing concentration (25%, 50%, 75%, and 100% (v/v)). Afterward, ethanol was removed with supercritical CO₂ in a Quorum K850 critical point dryer (Quorum Technologies Ltd., London, UK), and the samples were metalized using the Agar Auto Sputter Coater 103A (Agar Scientific Ltd., Stansted, UK), before being analyzed in a high-resolution ZEISS HD15 Scanning Electron Microscope (Zeiss, Oberkochen, Germany).