Anti myosin light chain 20 kda monoclonal antibody

Sphingosylphosphorylcholine (SPC) was purchased from Biomol (Plymouth Meeting, PA, USA). Tamoxifen was purchased from Funakoshi (Tokyo, Japan). Corn oil was purchased from Sigma Aldrich (St. Louis, MO, USA).
The following antibodies were used: mouse monoclonal anti-paxillin antibody (Cat#610620, BD Biosciences, San Jose, CA, USA), anti-paxillin polyclonal antibody (H85, Santa Cruz, Dallas, CA, USA), anti-GAPDH monoclonal antibody (Wako, Osaka, Japan), anti-phospho-MYPT1 (T850) with phosphorylation site corresponding to T853 in human MYPT1 (Milipore, Burlington, CA, USA), anti-MYPT1 antibody (H130, Santa Cruz, Dallas, CA, USA), anti-phospho-myosin light chain 2 (S19) monoclonal antibody (Cell Signaling, Danvers, MA, USA), anti-myosin light chain (20 kDa) monoclonal antibody (Sigma Aldrich, St. Louis, MO, USA), anti-α-smooth muscle actin antibody (Thermo Fisher, Waltham, MA, USA), anti-mouse Alexa Fluor 488 (Thermo Fisher, Waltham, MA, USA), and anti-rabbit Alexa Fluor 546 (Thermo Fisher,Waltham, MA, USA). Secondary HRP-labeled antibodies (anti-mouse and anti-rabbit) were purchased from Promega (Madison, WI, USA).

n-3 DPA (purity ≧ 98%) solution in 100% ethanol was obtained from Cayman Chemical (Ann Arbor, MI, USA). n-6 DPA (purity > 99%) was purchased from Nu-Chek Prep, Inc. (Elysian, MN, USA). DPA was dissolved in 100% ethanol to make a 30 mM stock solution. The stock solutions were stored at −20 °C, then diluted to final concentrations before use. Sphingosylphosphorylcholine (SPC) was purchased from Biomol (Plymouth Meeting, PA, USA). Fura-2/AM was purchased from Dojindo (Kumamoto, Japan). Bradykinin (BK) was purchased from Peptide institute, Inc. (Osaka, Japan). All other chemicals were purchased from Katayama Chemical (Osaka, Japan).
The following antibodies were used: anti-phospho-MYPT1 (Thr850) with phosphorylation site corresponding to Thr853 in human MYPT1 (Upstate), anti-GAPDH monoclonal antibody (Wako), anti-phospho-myosin light chain 2 (Ser19) monoclonal antibody (Cell Signaling), anti-myosin light chain (20 kDa) monoclonal antibody (Sigma Aldrich), anti-Rho-kinase-2 polyclonal antibody (H85, Santa Cruz), and anti-rabbit Alexa Fluor 488 (Thermo Fisher). Secondary HRP-labeled antibodies (anti-mouse and anti-rabbit) were purchased from Promega.