Focal cerebral ischemia in mice was performed as described previously [35 (link)]. In brief, adult male ICR mice weighing 25–30 g were anesthetized with ketamine/xylazine (100 mg/10 mg/kg; Sigma) intraperitoneally. Body temperature was maintained at 37 ± 0.5 °C using a heating pad (RWD Life Science, Shenzhen, China). Under the surgical microscope (Leica, Solms, Germany), the left common carotid artery (CCA), the external carotid artery (ECA), and the internal carotid artery (ICA) were isolated. Then a 6-0 suture (Dermalon, 1741-11; Covidien, OH, USA) with a round tip and coated with silicone was inserted from the ECA into the ICA and reached the circle of Willis to occlude the origin of the middle cerebral artery (MCA) until a slight resistance was felt. The distance from the furcation of the ECA/ICA to the opening of the MCA was 9 ± 0.5 mm. The success of occlusion was determined by monitoring the decrease in surface cerebral blood flow to 80% of baseline, which was verified by a laser Doppler flow-meter (Moor LAB; Moor Instruments, Devon, UK). Reperfusion was performed by withdrawing the suture 2 hours after middle cerebral artery occlusion (MCAO). To confirm successful occlusion/reperfusion, cerebral blood flow was tested again. The sham operated mice were subjected to the same procedure except for the suture insertion.
Moor lab
Manufactured by Moor Instruments
Sourced in United Kingdom
The Moor LAB is a versatile piece of laboratory equipment designed for various scientific applications. It offers core functionalities for data acquisition and analysis. The device is capable of measuring and recording relevant parameters, providing users with essential data for their research and experiments. Its compact design and intuitive interface make it a practical tool for laboratory settings.
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Lab products found in correlation
Heating pad, by RWD Life Science (3 mentions)
Surgical microscope, by Leica (1 mentions)
Ketamine xylazine, by Merck Group (1 mentions)
6 0 suture, by Medtronic (1 mentions)
Powerlab, by ADInstruments (1 mentions)
Zoletil, by Virbac (1 mentions)
Pressure transducer, by ADInstruments (1 mentions)
4 0 suture, by Medtronic (1 mentions)
Accurex coded, by Polar Electro (1 mentions)
10 protocols using moor lab
1
Focal Cerebral Ischemia Induction in Mice
2
Laser Doppler Monitoring of Stroke
3
Transient Middle Cerebral Artery Occlusion
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The surgical procedure for transient middle cerebral artery occlusion (tMCAO) has been previously described [15 (link)]. Briefly, animals were anesthetized with 1.5% isoflurane in a 30% O2/70% NO mixture under spontaneous breathing conditions on a heating pad (RWD Life Science, Shenzhen, China). A 6-0 suture (Covidien, Mansfield, MA, USA) coated with silicon was inserted through the external carotid artery (ECA) with an advancement of 0.9–0.95 mm from the internal carotid artery (ICA) until reaching the intersection of the middle cerebral artery (MCA). After 90 min of ischemia, the suture was withdrawn to allow reperfusion. A laser Doppler Monitor (Moor Lab, Moor Instruments, Devon, UK) was used to monitor the blood flow in the MCA territory before surgery, immediately after occlusion, and upon reperfusion. Successful occlusion of the MCA was defined as a decline in the regional blood flow of the ipsilateral hemisphere to 20% of the baseline. A recovery of cerebral blood flow to more than 70% of the baseline was regarded as a successful reperfusion. The mortality rate of mice that underwent tMCAO was 7.6%. Animals were randomly assigned into different groups. Both the surgeon and the behavioral analyst were blinded to treatment assignments.
4
Facial Nerve Blood Flow Measurement
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Two rats from each group at postoperative week 4 were anesthetized using an intraperitoneal injection of xylazine hydrochloride and tiletamine-zolazepam (Zoletil, Virbac, Carros, France). The recombinant position is more convenient for the measurement of FNBF when using Zoletil with xylazine hydrochloride. The main trunk of the facial nerve was carefully re-exposed, and the femoral artery was also identified. FNBF in the region was assessed using a laser Doppler blood flowmeter, as described previously [30 (link)]. The femoral artery, which is routinely used to measure systemic blood pressure (SBP), was cannulated, and a pressure transducer was attached (AD Instruments, Castle Hill, Sydney, NSW, Australia). On the main trunk of the FN, a 1.0 mm needle probe was positioned at a straight angle carefully to avoid nerve compression and coupled to a laser Doppler blood flowmeter (moorLAB, Moor Instruments, Axminster, Devon, UK). Every 20 s, data on the FNBF output and SBP were sampled and evaluated using a data acquisition program (PowerLab, AD Instruments) and a laptop (Samsung, Suwon, Republic of Korea). The FNBF was recorded for 30 min.
5
Cerebral Perfusion Monitoring After SAH
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rCBF monitoring was performed using a Laser Doppler device (moorLab, Moor Instruments, Axminster, Devon, United Kingdom) which measured relative rCBF (Flux) as well concentration (Conc) of moving blood cells. Laser Doppler probes were inserted into burr holes above the S1 and the cerebellar cortex and data collected 10 minutes before and for 30 minutes after SAH induction as well as at each of the following time points until euthanization: 6, 12, 24 and 72 hours after SAH (animals were re-anaesthetized for these later measurements). Five minutes of these recordings were evaluated and parameters expressed as relative to baseline. All Laser Doppler measurements were performed under general anesthesia. Analysis of cerebral perfusion data was performed blinded.
6
Middle Cerebral Artery Occlusion in Rats
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Wistar rats, belonging to the same strain with those used for collecting BMSC, were anesthetized by IP injection of choral hydrate (350 mg/kg). Body temperature was maintained at 37 ± 0.5°C using a heating pad (RWD Life Science, Shenzhen, China). An 4-0 suture (Covidien, Mansfield, MA, USA) with round tip and silicon coating was inserted from the left external carotid artery into the middle cerebral artery in rats (Yang and Betz, 1994 (link)). The success of the surgery was verified by detection the surface cerebral blood flow using a Laser Doppler flowmetry (Moor LAB, Moor Instruments, Devon, UK). After 2 h of suture insertion, the rats were anesthetized again and the suture was withdrawn to perform a reperfusion. The rats in the control group underwent a sham operation without suture insertion.
7
Transient MCAO with hUMSC-Exos Treatment
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As described previously [64 (link)], mice were anesthetized via intraperitoneal injection (35 mg/kg sodium pentobarbital). Transient middle cerebral artery occlusion (MCAO) was produced by advancing a 4-0 nylon monofilament (0.23–0.25 mm) (Yushun Bio Technology Co. Ltd., China) via the left common carotid artery to occlude the middle cerebral artery for 60 minutes, prior to filament withdrawal (reperfusion). Success of blood-flow occlusion and restoration was verified using a laser Doppler flowmeter (Moor LAB, Moor Instruments, Devon, UK). During the MCAO procedure, head temperature was maintained at 36° C. At four hours post-reperfusion, 250 μL PBS with or without 50 μg hUMSC-Exos was injected into the tail vein (experimental and vehicle-only groups). Mice in a third (control) group received no injection.
8
UVB-Induced Inflammation and Hyperalgesia
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The rats were briefly anesthetized with isoflurane 2% with 40% oxygen and 60% nitrous oxide. The distal lateral plantar part of the right hind paw area (contralateral to S1 probe) was irradiated with UVB light (Fig. 4A ), while the rest of the paw was covered with a UVB opaque material. The UVB source consisted of a set of four TL/01 fluorescent tubes (between λ = 305 to 315 nm, kmax = 311 nm, double pins spaced 2.8 cm; Phillips, UK), producing an even field of irradiation. The UVB lamp was calibrated before each experiment using a Variocontrol meter (Herbert Waldmann GmbH & Co. KG, Germany). The UVB lamp was positioned 3 cm above the hindlimb. The UVB dose was adjusted for each rat to be 1.3 mJ/cm2, an intensity known to produce robust hyperalgesia but below the blistering threshold (45 (link)). A laser Doppler flow meter (Moor-LAB, Moor Instruments, UK) was used to measure the skin blood flow before and 2 days after irradiation in all rats, confirming robust induction of skin inflammation (Fig. 5B ).
9
Thermoregulation During Exercise Trials
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At least 72 h separated the trials and subjects were tested in the morning. 4 h before arriving at the laboratory, participants ingested a telemetric thermistor pill (CorTemp™, HQ, Inc., Palmetto, Florida, USA) for the measurement of intestinal temperature (T INT ). 2 h before arrival to the laboratory subjects ingested a light breakfast of 500 kcal and 65 g of carbohydrate. Upon arrival to the laboratory subjects voided and nude body weight was recorded (Toledo Hawk, Mettler, Columbus, OH, USA). Urine specific gravity (U SG ) was measured to confirm euhydration (U SG < 1.020; [45] ). Then subjects were instrumented with an ECG-gated automated blood pressure sphygmomanometer (Tango™ SunTech Medical, Inc., Morrisville, NC, USA) and a telemetric heart rate monitoring device (Accurex coded, Polar, Finland). Thereafter subjects lay on a stretcher and a laser Doppler flowmeter (Moor Lab, Moor Instruments, Devon, UK) was attached to the dorsal side of the forearm to measure skin blood flow (S K BF). Data was expressed as percent of maximal vasodilation elicited at the end of the trial by local heating to 42 °C. After 45 min of supine rest, blood pressure, metabolic and cardiovascular measurements were collected before and after exercise. At the end of exercise, subjects toweled dry and nude weight was measured again.
10
Quantifying Cutaneous Blood Flow
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Cutaneous blood flow, measured as red blood cell flux, was used as another index of erythema. 24 hours after UVB challenge, the cutaneous blood flow at each irradiated site and adjacent non-irradiated skin was measured by a laser Doppler perfusion monitor (MoorLAB, Moor Instruments Ltd., Axminster, UK). The perfusion monitor with two satellite units connected to the server allowed flux readings from three laser probes to be recorded simultaneously. The perfusion monitor was linked to a computer and recordings displayed continuously by MoorSoft v1.31 for Windows. During the measurement, the three laser probes were secured to the overlying test sites of the skin using a ring of double sided adhesive tape and data were collected for at least 10 minutes, from which an average flux was calculated.
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