Following H&E staining, slides were imaged using a Zeiss Axio Observer 7 microscope (Zeiss, 4310079904) with an Axiocam 506 color camera (Zeiss, 426556). For gene expression slides (10X Genomics, PN 2000233), stained sections without coverslips were imaged using an LD Plan-Neofluar 20X/0.4na objective (Zeiss, 4213519972) and stained sections with coverslips were imaged with a Plan-Apochromat 20X/0.8na objective (Zeiss, 4406409903). For tissue optimization slides (10X Genomics, PN 3000394), stained sections were imaged with a Plan-Apochromat 10X/0.45na objective (Zeiss, 4206419910). Following tissue removal, fluorescence imaging of tissue optimization slides was performed with the same microscope, using an Axiocam 506 mono camera (Zeiss, 426557), Plan-Apochromat 10X/0.45na objective (Zeiss, 4206419910), Solid-state light source Colibri 7, type RYB-UV light source (Zeiss, 4230529720), and the Zeiss filter set 20 HE (Zeiss, 489020000).
Plan apochromat 10x 0.45na objective
Manufactured by Zeiss
The Plan-Apochromat 10X/0.45na objective is a high-performance microscope objective lens manufactured by Zeiss. It is designed to provide superior optical performance with a high numerical aperture of 0.45 and a long working distance. The lens is plan-apochromatic, which ensures consistent image quality and flatness of field across the entire field of view.
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Lab products found in correlation
Axiocam mrc camera, by Zeiss (1 mentions)
Axioskop2 mot plus microscope, by Zeiss (1 mentions)
Lsm 710 fcs confocal microscope, by Zeiss (1 mentions)
Zen software, by Zeiss (1 mentions)
Axiocam 506 color camera, by Zeiss (1 mentions)
Axio observer 7 microscope, by Zeiss (1 mentions)
Axiocam 506 mono camera, by Zeiss (1 mentions)
Plan apochromat 20x 0.8 n a objective, by Zeiss (1 mentions)
2 protocols using plan apochromat 10x 0.45na objective
1
Optimizing Microscopic Imaging for Tissue Analysis
2
Microscopic Imaging Protocol for Biological Samples
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Stainings were examined using an Axioskop2 mot plus microscope (Carl Zeiss, Inc.), equipped with an AxioCamMRc camera and a Plan-APOCHROMAT 10x/0.45 NA objective (Carl Zeiss, Inc.) Images were acquired using Axio Vision Version 4.4 software (Carl Zeiss). Confocal images were acquired with a LSM710 FCS confocal microscope and ZEN software (Zeiss, Jena, Germany) and were processed with IMARIS software (Verstion 7.1.1, Bitplane AG, Zürich, Switzerland).
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