Quantikine hs elisa human il 6 immunoassay hs600b

Serum samples were frozen and stored at −80°C at the laboratory of nephrology of our Institution. Values were evaluated in duplicate by using enzyme-linked immunosorbent assay (ELISA) kits following the manufacturer’s instructions. We used these specific kits: Quantikine ELISA Human CCL2/MCP-1 Immunoassay DCP00, Human TNF-alpha ELISA Kit (Thermo Fisher Scientific, Monza, Italy), Quantikine HS ELISA Human IL-6 Immunoassay HS600B (R&D Systems, Space, Milano, Italy). Zero was included in each resulting curve as the last standard value. Results were validated by using Quantikine Immunoassay Control Group 1–4 or 10 (R&D Systems, Space, Milano, Italy). Absorbance readings were measured at 450 nm by spectrophotometer (Xenius Safas, Monaco).

Serum cytokines concentration were dosed in duplicate using the following ELISA kits according to the manufacturer’s instructions:
Quantikine ELISA Human CCL2/MCP-1 Immunoassay DCP00, Human TNF-alpha ELISA Kit (Thermo Fisher Scientific, Monza, Italy) and Quantikine HS ELISA Human IL-6 Immunoassay HS600B (R&D Systems, Space, Milano, Italy). Zero was included, as the last standard value, in each resulting curve. Results were validated by using Quantikine Immunoassay Control Group 1–4 or 10 (R&D Systems, Space). Absorbance readings were measured at 450 nm by spectrophotometer (Xenius Safas, Monaco).