Donkey anti rabbit igg na9340v

Manufactured by GE Healthcare

Donkey anti–rabbit IgG (NA9340V) is a secondary antibody used in immunoassays and other applications where the detection of rabbit primary antibodies is required. It is produced in donkeys and purified to react specifically with rabbit immunoglobulin G (IgG) antibodies.

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Lab products found in correlation

Enhanced chemi luminescence (ecl), by GE Healthcare (2 mentions) Ab110413, by Abcam (2 mentions) Sheep anti mouse nxa931, by GE Healthcare (2 mentions) Pvdf membrane, by GE Healthcare (1 mentions) A5316, by Merck Group (1 mentions) Ab104274, by Abcam (1 mentions) Ab56889, by Abcam (1 mentions) Mabn504, by Merck Group (1 mentions) Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions) Ab15246, by Abcam (1 mentions)

Spelling variants of this product

NA9340V (28 citations) Anti-rabbit # NA9340V (2 citations)

2 protocols using donkey anti rabbit igg na9340v

Mitochondrial Protein Analysis by Western Blot

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Proteins from mitochondrial and whole cell lysates were separated by SDS-PAGE and blotted onto PVDF membranes (GE Healthcare). The following primary antibodies were used: mouse anti–β-actin (1:5,000, A5316; Sigma-Aldrich), rabbit anti-Mitofusin1 (1:1,000, ab104274; Abcam), mouse anti-MFN2 (1:1,000, ab56889; Abcam), mouse anti-VDAC (1:5,000, MABN504; EMD Millipore), and mouse anti-OXPHOS antibody cocktail (1:1,000, ab110413; Abcam). The following secondary antibodies were used: donkey anti–rabbit IgG (NA9340V; GE Healthcare) and sheep anti–mouse (NXA931; GE Healthcare). Detection was done by incubation with HRP-conjugated secondary antibodies and conversion to chemiluminescence with ECL (GE Healthcare).

Mourier A., Motori E., Brandt T., Lagouge M., Atanassov I., Galinier A., Rappl G., Brodesser S., Hultenby K., Dieterich C, & Larsson N.G. (2015). Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. The Journal of Cell Biology, 208(4), 429-442.

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Mitochondrial Protein Immunoblotting Protocol

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Proteins from mitochondrial and whole-cell lysates were separated by SDS-PAGE and blotted onto polyvinylidene fluoride membranes (GE Healthcare). The following primary antibodies were used: rabbit anti-α-actin (1:5,000, ab15246; Abcam), mouse anti-COQ6 (1:100, sc-393932; Santa Cruz Biotechnology), mouse anti-COQ9 (1:1,000, sc-271892; Santa Cruz Biotechnology), mouse anti-COQ5 (1:500, 17453-1-AP; Proteintech), and mouse anti-OXPHOS antibody cocktail (1:1,000, ab110413; Abcam). The following secondary antibodies were used: donkey anti-rabbit IgG (NA9340V; GE Healthcare) and sheep anti-mouse (NXA931; GE Healthcare). Detection was done by incubation with HRP-conjugated secondary antibodies and conversion to chemiluminescence with ECL (GE Healthcare).

Pujol C., Legrand A., Parodi L., Thomas P., Mochel F., Saracino D., Coarelli G., Croon M., Popovic M., Valet M., Villain N., Elshafie S., Issa M., Zuily S., Renaud M., Marelli-Tosi C., Legendre M., Trimouille A., Kemlin I., Mathieu S., Gleeson J.G., Lamari F., Galatolo D., Alkouri R., Tse C., Rodriguez D., Ewenczyk C., Fellmann F., Kuntzer T., Blond E., El Hachimi K.H., Darios F., Seyer A., Gazi A.D., Giavalisco P., Perin S., Boucher J.L., Le Corre L., Santorelli F.M., Goizet C., Zaki M.S., Picaud S., Mourier A., Steculorum S.M., Mignot C., Durr A., Trifunovic A, & Stevanin G. (2021). Implication of folate deficiency in CYP2U1 loss of function. The Journal of Experimental Medicine, 218(11), e20210846.

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