Immunohistochemical staining of neonatal mouse brain sections to detect GFP was performed as previously described (Rahim et al., 2012 (link)) using the following antibodies: anti-GFP (1:4000, ab290, Abcam), biotinylated secondary antibody (1:1000, BA-1000, Vector Laboratories) (Supplementary material ). Representative images were captured using a live video camera (Nikon, DS-Fil) mounted onto a Nikon Eclipse E600 microscope.
For immunohistochemical analysis in adult rat brains, a standard free-floating protocol was used with an anti-GFP primary antibody (1:20 000, ab13970, Abcam). Biotinylated secondary antibody (1:250, BA-9010, Vector Laboratories) was used for DAB immunohistochemistry and amplified by Vector Labs ABC kit (Supplementary material ). Images were captured using an Olympus BX53 microscope and analysed using cellSens Dimension v. 1.11 software.
For Isolectin B4 (ILB4) staining in adult mouse brains, coronal sections (30 µm) were stained as previously described (Wilkinson et al., 2012 (link)).
For immunohistochemical analysis in adult rat brains, a standard free-floating protocol was used with an anti-GFP primary antibody (1:20 000, ab13970, Abcam). Biotinylated secondary antibody (1:250, BA-9010, Vector Laboratories) was used for DAB immunohistochemistry and amplified by Vector Labs ABC kit (
For Isolectin B4 (ILB4) staining in adult mouse brains, coronal sections (30 µm) were stained as previously described (Wilkinson et al., 2012 (link)).