Dp controller image software

A high-voltage power supply (Series 225, Bertan High Voltage Corp., Hicksville, NY, USA) was employed to provide the required voltages for both junction gap breakdown and protein preconcentration. After the nanofractures were created, the resistance difference between the junction gaps before and after the application of a DC voltage for electric breakdown was measured under various frequencies using an LCR meter (4620A, Wayne Kerr, Woburn, MA, USA). The preconcentration of proteins was observed and recorded using an inverted fluorescence microscope (CKX41, Olympus, Tokyo, Japan) with a mounted CCD camera (DP71, Olympus, Tokyo, Japan) and connected to a computer with Olympus DP Controller image software. Quantification of the fluorescent intensities emitted by enriched FITC-labeled BSA was performed using NIH ImageJ software (National Institutes of Health, Bethesda, Maryland, USA), which has the ability to assess the density of each pixel.

A high-voltage power supply (Series 225, Bertan High Voltage Corp., Hicksville, NY, USA) was employed to provide the required voltages for both junction gap breakdown and protein preconcentration. The I-V curves for the region between the junction gaps before and after the application of a DC voltage for electric breakdown were measured using an Agilent B2902A Precision Source/Measurement Unit. Protein preconcentration was observed and recorded using an inverted fluorescence microscope (CKX41, Olympus, Tokyo, Japan) with a mounted CCD camera (DP71, Olympus, Tokyo, Japan) and connected to a computer running Olympus DP Controller image software. The fluorescence intensities emitted by enriched FITC-labeled BSA were quantified using ImageJ software (National Institutes of Health, Bethesda, Maryland, USA), which can assess the density of each pixel.