Blood samples were obtained by venepuncture and collected in EDTA tubes. They were centrifuged to isolate plasma, aliquoted and stored at −70°C until further analyses. Plasma assays were conducted at the UK Dementia Research Institute biomarker laboratory. Plasma samples were thawed on wet ice, centrifuged at 500× g for 5 min at 4°C. Calibrators (neat) and samples (plasma: 1:4 dilution) were measured in duplicates. The plasma assays measured were the Quanterix Simoa Human Neurology 4-Plex E assay (measuring Aβ40, Aβ42, GFAP and NfL) and the Quanterix Simoa p-tau181 measuring p-tau181 of the human tau protein. Assays were performed using the Simoa-HD1 according to the manufacturer’s protocol (Quanterix Corp, Billerica, Massachusetts, USA) (Rissin et al). All samples were analysed at the same time using the same batch of reagents. A four-parameter logistic curve fit data reduction method was used to generate a calibration curve. Two control samples of known concentration of the protein of interest (high-control and low-control) were included as quality control. The mean coefficient of variation percentage for p-tau181 was 6.29, for NfL 4.08, for Aβ42 3.06, for Aβ40 2.55 and for GFAP 4.12.
Simoa p tau181
Manufactured by Quanterix
The Simoa p-tau181 is a quantitative assay that measures the phosphorylated form of tau protein at threonine 181 (p-tau181) in biological samples. The assay utilizes Simoa technology, which enables the detection and quantification of low-abundance proteins with high sensitivity.
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Lab products found in correlation
2 protocols using simoa p tau181
1
Plasma Biomarker Quantification Protocol
2
Plasma Biomarker Quantification Protocol
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Blood samples were obtained by venepuncture and collected in EDTA tubes. They were centrifuged to isolate plasma, aliquoted and stored at −70°C until further analyses. Plasma assays were conducted at the UK Dementia Research Institute biomarker laboratory. Plasma samples were thawed on wet ice, centrifuged at 500× g for 5 min at 4°C. Calibrators (neat) and samples (plasma: 1:4 dilution) were measured in duplicates. The plasma assays measured were the Quanterix Simoa Human Neurology 4-Plex E assay (measuring Aβ40, Aβ42, GFAP and NfL) and the Quanterix Simoa p-tau181 measuring p-tau181 of the human tau protein. Assays were performed using the Simoa-HD1 according to the manufacturer’s protocol (Quanterix Corp, Billerica, Massachusetts, USA) (Rissin et al). All samples were analysed at the same time using the same batch of reagents. A four-parameter logistic curve fit data reduction method was used to generate a calibration curve. Two control samples of known concentration of the protein of interest (high-control and low-control) were included as quality control. The mean coefficient of variation percentage for p-tau181 was 6.29, for NfL 4.08, for Aβ42 3.06, for Aβ40 2.55 and for GFAP 4.12.
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