The largest database of trusted experimental protocols

2 protocols using anti h pgds

1

Toll-like Receptor Signaling in Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pam3CSK4 (TLR1/2) and LPS (TLR4) were obtained from InvivoGen (Carlsbad, CA, USA). Mouse recombinant IFN-β was purchased from PBL InterferonSource (now PBL Assay Science, Piscataway, NJ, USA) and mouse IFN-γ was from R&D Systems (Minneapolis, MN, USA). Antibodies used in this study were obtained as follows. Anti-H-PGDS and Anti-COX-2 antibodies were produced by Cayman Chemical (Ann Arbor, MI, USA). Anti-STAT1 and anti-pY701-STAT1 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-actin antibody was from BD Biosciences (Franklin Lakes, NJ, USA). PGs were purchased from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were from Sigma (St. Louis, MO, USA), unless stated otherwise.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Tissue Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
After deparaffinization, the sections were antigen-activated with Histo VT One (Nacalai Tesque) and blocked using Blocking One Histo (Nacalai Tesque). Primary antibody reactions were performed with anti-HPGDS (Cayman Chemicals, Ann Arbor, MI, USA) at a dilution ratio of 1:2000 in Solution A (Toyobo) and anti-Tryptase (ab2378: Abcam), anti-NG2 (sc-80003: Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-CD11b (66519-1-Ig: Proteintech Group, Inc., Rosemont, IL, USA), anti-Troponin T (MAB18742: Bio-Techne Corp., Minneapolis, MN, USA), anti-α-SMA (sc-53142: Santa Cruz Biotechnology, Inc.), and anti-CD31 (sc-376764: Santa Cruz Biotechnology, Inc.) at a dilution ratio of 1:500 in Solution A overnight at 4 °C. Secondary antibodies were reacted with goat anti-rabbit IgG H&L (Alexa Fluor® 647) (ab150083: Abcam) for anti-HPGDS and goat anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113: Abcam) for others in Solution A (Toyobo) (dilution 1:1200) for 1 h at room temperature. Sections were mounted with Vectashield Hard Set Mounting Medium with DAPI (H-1500: Vector Laboratories, Inc., Newark, CA, USA). To confirm the specificity of the antibodies, we also verified that no fluorescence was observed in the negative control without the primary antibody.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!