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Rabbit anti β2ar

Manufactured by Abcam
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Rabbit anti-β2AR is a primary antibody that specifically targets the beta-2 adrenergic receptor (β2AR), a G protein-coupled receptor involved in various physiological processes. This antibody is produced in rabbits and can be used for the detection and analysis of β2AR in various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation.

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Lab products found in correlation

Fix perm reagent, by BD (1 mentions) 100 μm cell strainer, by BD (1 mentions) Donkey anti rabbit bv421, by BioLegend (1 mentions) Bca protein assay reagent, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride pvdf membrane, by Thermo Fisher Scientific (1 mentions) Infrared labeled secondary antibody, by LI COR (1 mentions) Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions) Dnase, by Qiagen (1 mentions) β actin, by Merck Group (1 mentions) Protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Abcam (1 mentions) P creb, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Rabbit anti- (5 citations)

2 protocols using rabbit anti β2ar

1

Multiparameter Flow Cytometric Analysis of Lymphocytes

Cited 1 time
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Lymphocytes were isolated by passage through a 100 μm cell strainer (BD Biosciences, San Jose, CA). Single cell suspensions were subjected to red blood cell lysis by resuspension in ACK buffer. Following blocking of cell surface Fc receptors with anti-CD16/32, lymphocytes were surface stained using anti -CD4, -B220, -PD-1, -CXCR5. Cell viability was determined using Live/dead violet. Stromal cells were identified by staining with anti -CD45-PE, -CD31-PE-Cy7 in stain buffer (20 min, 4°C, Table 2). After extensive washing to remove unbound antibodies, cells were subjected to fixation and permeabilization using Fix/Perm reagent according to manufacturer’s instructions (BD Biosciences). Intracellular staining was performed using rabbit anti-β2AR (Abcam) [33 (link)–35 (link)] followed by donkey anti-rabbit BV421 (Biolegend, San Diego, CA) and goat anti-CXCL13 (RnD Systems, Minneapolis, MN) followed by donkey anti-goat Alex Fluor 647 (Invitrogen). After extensive washing to remove unbound antibodies, flow cytometry was performed on a LSRII running DIVA 6.0 (BD Biosciences). Data was analyzed using FlowJo v10.0 (Treestar, Ashland, Oregon).
Murray K., Godinez D.R., Brust-Mascher I., Miller E.N., Gareau M.G, & Reardon C. (2017). Neuroanatomy of the spleen: Mapping the relationship between sympathetic neurons and lymphocytes. PLoS ONE, 12(7), e0182416.
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2

RSV Infection of HASMCs: Signaling Mechanisms

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HASMCs at 70 to 80% confluency were infected with rrRSV (at an MOI of 1) at the times indicated in the figure legends. Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing 25 mM tris-HCl (pH 7.6), 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS (Thermo Fisher Scientific), protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific), and DNase (Qiagen). Protein concentration was determined with the bicinchoninic acid (BCA) protein assay reagent (Thermo Fisher Scientific). Proteins were resolved by SDS–polyacrylamide gel electrophoresis (PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Thermo Fisher Scientific). The membranes were blocked in tris-buffered saline/Tween 20–5% bovine serum albumin for 1 hour at room temperature and incubated with the following primary antibodies: rabbit anti-β2AR (Abcam), rabbit anti-pβ2AR (355,356; Santa Cruz Biotechnology), CREB (Cell Signaling Technology), pCREB (Ser133; Cell Signaling Technology), AC (GeneTex), and RSV-G protein (GeneTex). β-Actin (Sigma-Aldrich) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam) was used to ensure equal loading. Luminol-generated signals (Thermo Fisher Scientific) or infrared-labeled secondary antibodies (LICOR) were used to detect the bands.
Harford T.J., Rezaee F., Gupta M.K., Bokun V., Naga Prasad S.V, & Piedimonte G. (2021). Respiratory syncytial virus induces β2-adrenergic receptor dysfunction in human airway smooth muscle cells. Science signaling, 14(685), eabc1983.
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