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Mock igg control

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The Mock IgG control is a laboratory reagent used as a negative control in immunological experiments. It consists of a nonspecific immunoglobulin G (IgG) antibody that does not bind to any known target antigen. This control is used to establish baseline signals and distinguish specific from nonspecific binding in assays such as Western blotting, immunoprecipitation, and flow cytometry.

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Lab products found in correlation

Anti zeb1 antibody, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Mock IgG Control IgG

2 protocols using mock igg control

1

Chromatin Immunoprecipitation to Analyze ZEB1 Binding

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Chromatin immunoprecipitation assays were performed as previously described (32 (link), 35 (link)). Immunoprecipitation was carried out using an anti-ZEB1 antibody or mock IgG control (Santa Cruz, Dallas, TX). Promoter segment enrichment was analyzed by qPCR using primers (Supplementary methods) flanking the potential ZEB1 binding sites along the LOXL2 promoter.
Peng D.H., Ungewiss C., Tong P., Byers L.A., Wang J., Canales J.R., Villalobos P.A., Uraoka N., Mino B., Behrens C., Wistuba I.I., Han R.I., Wanna C.A., Fahrenholtz M., Grande-Allen K.J., Creighton C.J, & Gibbons D.L. (2016). ZEB1 Induces LOXL2-Mediated Collagen Stabilization and Deposition in the Extracellular Matrix to Drive Lung Cancer Invasion and Metastasis. Oncogene, 36(14), 1925-1938.
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2

Chromatin Immunoprecipitation to Analyze ZEB1 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromatin immunoprecipitation assays were performed as previously described (32 (link), 35 (link)). Immunoprecipitation was carried out using an anti-ZEB1 antibody or mock IgG control (Santa Cruz, Dallas, TX). Promoter segment enrichment was analyzed by qPCR using primers (Supplementary methods) flanking the potential ZEB1 binding sites along the LOXL2 promoter.
Peng D.H., Ungewiss C., Tong P., Byers L.A., Wang J., Canales J.R., Villalobos P.A., Uraoka N., Mino B., Behrens C., Wistuba I.I., Han R.I., Wanna C.A., Fahrenholtz M., Grande-Allen K.J., Creighton C.J, & Gibbons D.L. (2016). ZEB1 Induces LOXL2-Mediated Collagen Stabilization and Deposition in the Extracellular Matrix to Drive Lung Cancer Invasion and Metastasis. Oncogene, 36(14), 1925-1938.
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