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Creatinine

Manufactured by Randox
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Sourced in United Kingdom

Creatinine is a laboratory equipment product that measures the concentration of creatinine, a waste product from the normal breakdown of muscle tissue, in the blood or urine. It is a key test used to evaluate kidney function.

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Lab products found in correlation

Urea, by Randox (8 mentions) Glutathione (gsh), by Merck Group (5 mentions) Bilirubin, by Randox (4 mentions) Total bilirubin, by Randox (4 mentions) 1 chloro 2 4 dinitrobenzene cdnb 5 5 dithiobis 2 nitrobenzoic acid dtnb epinephrine, by Merck Group (4 mentions) Hydrogen peroxide h2o2, by Merck Group (4 mentions) Hydrogen peroxide, by Merck Group (3 mentions) Uric acid, by Randox (3 mentions) Total cholesterol, by Randox (2 mentions) Hdl cholesterol, by Randox (2 mentions) Ldl cholesterol, by Randox (2 mentions) Glutathione gsh 1 chloro 2 4 dinitrobenzene cdnb 5 5 dithiobis 2 nitrobenzoic acid dtnb thiobarbituric acid tba epinephrine, by Merck Group (2 mentions) Sulphosalicylic acid, by Merck Group (1 mentions) Lead acetate, by Merck Group (1 mentions) O dianisidine, by Merck Group (1 mentions) Xanthine, by Merck Group (1 mentions) Furan, by Merck Group (1 mentions) Dcfh da, by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) Griess reagent, by Merck Group (1 mentions)

16 protocols using creatinine

1

Albino Rat Biochemical Assays

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Twenty-four albino male rats (Ratus novergicus) with an average weight of 140 g used for this study were purchased from the Animal Holding Unit of the Department of Biochemistry, University of Ibadan, Nigeria. ATR-MET is a product of First Chemical Co Ltd, Changxing, Zhejiang China. GSH, 1-chloro-2, 4-dinitrobenzene (CDNB), 5′, 5′-dithio-bis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). ALT, AST, ALP, GGT, urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides kits were obtained from Randox Laboratories Ltd, Antrim UK. All other chemicals and reagent were of analytical grade and were obtained from British Drug House, Poole, UK.
Olayinka E.T., Ore A., Adewole K.E, & Oyerinde O. (2022). Evaluation of the toxicological effects of atrazine-metolachlor in male rats: in vivo and in silico studies. Environmental Analysis, Health and Toxicology, 37(3), e2022021.
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2

Hepatorenal Biomarkers Quantification Protocol

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The chemicals, reagents and kits used to determine the hepatorenal, redox, inflammatory and apoptotic biomarkers in the liver and kidney of rats following subacute exposure of rats to PbAc and Furan were purchased from recognised chemical companies. Specifically, Lead Acetate, Furan, TBA: thiobarbituric acid, DCFH-DA: 2’, 7’-dichlorodihydrofluorescin diacetate, DTNB: 5’, 5’-dithiobis-2-nitrobenzoic acid, CDNB: 1-chloro-2,4-dinitrobenzene, H2O2: hydrogen peroxide, KCl: potassium chloride, TCA: trichloroacetic acid, sodium azide, GSH: glutathione, epinephrine, sulphosalicylic acid, xanthine, Griess reagent, and O-dianisidine were purchased from Sigma-Aldrich Chemical (MO, USA); ALT: Alanine aminotransferase, AST: Aspartate aminotransferase, ALP: Alkaline phosphatase, urea, and creatinine were bought from Randox™ Laboratories Limited, (Crumlin, UK); and IL-1β: Interleukin 1-beta, IL-10: interleukin-10, 8-OHdG: 8-hydroxydeoxyguanosine, caspase-9 and caspase-3 were bought from Elabscience Biotechnology Company (Wuhan, China).
Owumi S.E., Arunsi U.O., Oyewumi O.M, & Altayyar A. (2022). Accidental lead in contaminated pipe-borne water and dietary furan intake perturbs rats’ hepatorenal function altering oxidative, inflammatory, and apoptotic balance. BMC Pharmacology & Toxicology, 23, 76.
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3

Comprehensive Biomarker Analysis of Renal Function

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Concentrations of Creat, BUN, minerals (sodium (Na+), potassium (K+), calcium (Ca2+), and phosphate (PO43-), albumin, ALT, and ALP, were determined using a VetScan reagent rotor (Comprehensive Diagnostic Profile, VetScan VS2, Abaxis, Ca, USA). Biomarkers of renal damage, including CysC (cystatin C), Clu (clusterin), and NGAL (neutrophil gelatinase-associated lipocalin), were measured by species-specific ELISA (canine cystatin C, Biovendor, Czech Republic; canine clusterin, Biovendor, Czech Republic; dog NGAL, Bioporto, Denmark). Urinary Creat was assayed using an enzymatic colorimetric kit (Creatinine, Randox Laboratories, UK).
Martineau A.S., Leray V., Lepoudere A., Blanchard G., Bensalem J., Gaudout D., Ouguerram K, & Nguyen P. (2016). A mixed grape and blueberry extract is safe for dogs to consume. BMC Veterinary Research, 12, 162.
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4

Mouse Inflammation and Biomarker ELISA Protocol

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Duoset mouse Lcn2 and CXCL1 (KC) ELISA kit, and anti-mouse Lcn2 antibody were obtained from R&D Systems. Aspartate transaminase (AST), alanine transaminase (ALT) triglycerides (TG), cholesterol (CHOL), lactate dehydrogenase (LDH) and creatinine kits were procured from Randox Laboratories. Glucometer and glucose test strips were obtained from Nova Max. Iron atomic absorption (AA) standard was purchased from RICCA Chemical Company. Ferrous sulfate was obtained from Alfa Aesar. SYBR® Green mix and qScript cDNA synthesis kit was procured from Quanta Biosciences. Bleomycin sulfate was procured from Biotang. Reagent grade Dextran Sulfate Sodium salt (DSS, reagent grade, M.W. 36–50 kDa, Ref = 160110) was purchased from MP Biomedicals. Mouse recombinant (rec)-Lcn2 was obtained from Cell Signaling and human rec-NGAL was acquired from R&D Systems; both of which are free from endotoxin, siderophore, and iron. All other fine chemicals used in present study were reagent grade and procured from Sigma.
Xiao X., Yeoh B.S., Saha P., Olvera R.A., Singh V, & Vijay-Kumar M. (2016). Lipocalin 2 Alleviates Iron Toxicity by Facilitating Hypoferremia of Inflammation and Limiting Catalytic Iron Generation. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 29(3), 451-465.
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5

Blood Serum Collection and Analysis

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The procedure described by Yakubu and Akanji (2011) was adopted for the preparation of serum. The animals were sacri ced by cervical dislocation and the blood collected by direct heart punctured into EDTA sample bottles and spinned at 3000rpm for 20mins. The serum of both control and treated animals were carefully aspirated with Pasteur pipette into sample bottles for the various biochemical assays which are Alkaline phosphate (ALP), Aspartate aminotranferase (AST), Alanine amino tranferase (ALT), urea, total bilirubin, direct bilirubin and creatinine were products of Randox Laborites Limited, United Kingdom.
Olorunsogbon S.T., Akinneye J.O., & Olowu E.A. (2021). Fumigant Toxicity of Cleistopholis patens (Benth) Oil extracts on Plodia interpunctella (Hubner) and Its Toxicological Effects in Wistar Rats.
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6

Methotrexate-Induced Oxidative Stress

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Methotrexate tablets is a product of West Coast Pharmaceutical Works Ltd, Gota, Ahmedabad, India; Gallic acid, Glutathione, 1-chloro-2,4-dinitrobenzene (CDNB),5,5 -dithio bis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2), were obtained from Sigma® Chemical Company, London, UK; Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), urea, Creatinine, total bilirubin were purchased from Randox® Laboratories Ltd. (Antrim, UK). All other reagents used were of analytical grade and of highest purity.
Olayinka E.T., Ore A., Adeyemo O.A, & Ola O.S. (2016). Ameliorative Effect of Gallic Acid on Methotrexate-Induced Hepatotoxicity and Nephrotoxicity in Rat. Journal of Xenobiotics, 6(1), 6092.
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7

Moxifloxacin-Induced Oxidative Stress

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Moxifloxacin hydrochloride (Avelox) was a product of Bayer Healthcare Pharmaceutics, Leverkusen, Germany. Glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), 5′,5′-dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine, and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT), urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides were products of Randox Laboratories Ltd., Antrim, UK. All other chemicals and reagents used in this study were of analytical grade.
Ore A, & Olayinka E.T. (2015). Influence of Moxifloxacin on Hepatic Redox Status and Plasma Biomarkers of Hepatotoxicity and Nephrotoxicity in Rat. Biochemistry Research International, 2015, 192724.
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8

Melphalan-Induced Hepatotoxicity Study

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Melphalan tablets were a product of Excella GmbH, Nürnberger, Germany. Quercetin, glutathione, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2) were all purchased from Sigma Chemical Company (London, UK). Kits for alanine transferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT), urea, creatinine, and total bilirubin were products of Randox laboratories Ltd. (Antrim, UK). All other reagents used were of analytical grade and of the highest purity.
Olayinka E.T., Ore A., Ola O.S, & Adeyemo O.A. (2014). Protective Effect of Quercetin on Melphalan-Induced Oxidative Stress and Impaired Renal and Hepatic Functions in Rat. Chemotherapy Research and Practice, 2014, 936526.
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9

Cyclophosphamide Hepatotoxicity Evaluation

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Cyclophosphamide tablet is a product of West Coast Pharmaceutical Works Ltd, Gota, Ahmedabad, India. Glutathione, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), epinephrine, and hydrogen peroxide (H2O2) were all purchased from Sigma Chemical Company (London, UK). Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), Urea, Creatinine and total Bilirubin were obtained from Randox laboratories ltd. (Antrim, UK). All other reagents used were of analytical grade and of highest purity.
Olayinka E.T., Ore A., Ola O.S, & Adeyemo O.A. (2015). Ameliorative Effect of Gallic Acid on Cyclophosphamide-Induced Oxidative Injury and Hepatic Dysfunction in Rats. Medical Sciences, 3(3), 78-92.
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10

Sesamin-Cyclophosphamide Nephroprotective Assay

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Sesamin, cyclophosphamide, 5,5dithiobis-(2-nitrobenzoic acid), reduced GSH, TBA, were obtained from Sigma chemicals (3300 S 2nd St #3306 St. Louis, MO 63118, USA). Biochemical assay kits for BUN, Uric Acid, and Creatinine were obtained from Randox, Crumlin, UK. Elisa estimation kits for IL-1β, TNFα, and Caspase-3 were purchased from Abcam, Cambridge, UK.
Alshahrani S., Ali Thubab H.M., Ali Zaeri A.M., Anwer T., Ahmed R.A., Jali A.M., Qadri M., Nomier Y., Moni S.S, & Alam M.F. (2022). The Protective Effects of Sesamin against Cyclophosphamide-Induced Nephrotoxicity through Modulation of Oxidative Stress, Inflammatory-Cytokines and Apoptosis in Rats. International Journal of Molecular Sciences, 23(19), 11615.
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