Nb100 305

Antibody recognizing 53BP1 (A300-272A) and RPA32 pS (4/8) (IHC-00422) were purchased from Bethyl Laboratories (Montgomery, TX). The phospho-specific γ-H2AX antibody (JBW301) was obtained from Millipore (Billerica, MA). Mre11 (12D7), Ku70 (GTX233114) and Ku80 (GTX70485) were purchased from Genetex. Actin (C-11), BRCA1 (sc-642). RNAPII (sc-899), ATR pS 428 (sc-109912) and Rad51 (H-92) antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA). Total (2662) and pT68 Chk2 (2661) antibodies and total (2360) and pS317 (2344) Chk1 were purchased from Cell Signaling. S9.6, an antibody specific for R loops (RNA:DNA hybrids) [37 (link)], was provided by Dr. Stephen H. Leppla (NIH, Bethesda, MD). Antibodies used for ChIP: anti-BRCA1 (Gene-Tex, 6B4), anti-ATM (Novus Biologicals NB100-305), anti-γ-H2AX (Abcam, ab2893), anti-53BP1 (Novus Biologicals, NB100-305), anti-CtIP (Abcam) and anti-Gal4(DBD) (Santa Cruz Biotechnology, sc-577). Antibodies used in western blotting and SETX IP: anti-SETX (A301-105) from Bethyl Laboratories, anti-XRN2 (NBP1-68149) and anti-Rrp45 (NBP1-71702) from Novus Biologicals.

The following antibodies were used: TRF1 (Abcam, ab371; a generous gift from Dr. Titia de Lange) for Western blots, TRF1 (affinity-purified rabbit antibody against recombinant TRF1 from serum no. 605 448) (Grolimund et al. 2013 (link)) for IF, TRF2 (Millipore, 05-521) for Western blots, γH2AX (Millipore, 05-636) for both Western blots and IF, hnRNPA1 (4B10; Santa Cruz Biotechnology, sc-32301) for Western blots, 53BP1 (Novus Biologicals, NB100-304) for IF, 53BP1 (Novus Biologicals, NB100-305) for Western blots, and phospho-ATM-Ser 1981 (Abcam, ab81292) for Western blots.