Il 17 ebio17b7

Manufactured by BD

The IL-17 (eBio17B7) is a lab equipment product. It functions as a reagent for the detection and quantification of interleukin-17 (IL-17) in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Facscanto instrument, by BD (2 mentions) Cd8 53 6, by BD (1 mentions) Cd4 rm4 5, by BD (1 mentions) Ifn γ xmg1.2, by BD (1 mentions) Mouse foxp3 buffer set, by BD (1 mentions)

Close spelling variants of this product

IL-17 (49 citations) EBio17B7 (5 citations)

2 protocols using il 17 ebio17b7

Comprehensive Lymphocyte Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymph nodes and spleen were disintegrated into single cells as previously described45 (link) and stained for surface markers with the following antibodies: TCR-β (H57-597), B220 (R43-6B2), CD4 (RM4-5), and CD8 (53-6.7), and all were purchased from BD Biosciences (Franklin Lakes, NJ, USA) or eBioscience (San Diego, CA, USA).
Spleen cells were stimulated as previously described46 , and then stained for intracellular cytokines using a Mouse FOXP3 Buffer Set (BD Biosciences). The following fluorochrome-conjugated antibodies were used: CD4 (GK1.5), CD25 (7D4), CD3 (145-2C11), FOXP3 (FJK-16s), RORγt (AFKJ-9), IFNγ (XMG1.2), and IL-17 (eBio17B7), and all were purchased from BD Biosciences or eBioscience.
Data was collected with a FACSCanto instrument (BD Biosciences).

Zhao M., Chen H., Ding Q., Xu X., Yu B, & Huang Z. (2016). Nuclear Factor Erythroid 2-related Factor 2 Deficiency Exacerbates Lupus Nephritis in B6/lpr mice by Regulating Th17 Cell Function. Scientific Reports, 6, 38619.

+ Open protocol

+ Expand

Spleen Cell Phenotyping and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The spleen tissue was disaggregated into single cells as described previously (24 ) and stained for 30 min in dark at room temperature for surface markers with the following antibodies: CD3 (554832; 1:100), CD4 (553651; 1:100) and CD8 (557085; 1:100) purchased from BD Biosciences (Franklin Lakes, NJ, USA). Spleen cells were stimulated and then stained for intracellular cytokines. RAW264.7 cells were treated as indicated above and then harvested for CD3 conjugation. The following fluorochrome-conjugated antibodies were used: CD4 (563933; 1:100 dilution, GK1.5), CD3 (561042; 1:100 dilution, 145-2C11), IL-17 (eBio17B7; 1:100 dilution) and RAR-related orphan receptor (ROR)γt (12-6988-82; 1:100 dilution, AFKJ-9), and all were purchased from BD Biosciences or eBioscience (Thermo Fisher Scientific, Inc.). The antibodies were stained in dark for 30 min Finally, the labeled cells were quantified with a FACSCanto instrument (BD Biosciences).

Xu S.P, & Li Y.S. (2018). Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation. International Journal of Molecular Medicine, 42(6), 3220-3230.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!